NMR Assignments, Secondary Structure and Hydration of Oxidized Escherichia coli Flavodoxin

1997 ◽  
Vol 244 (2) ◽  
pp. 384-399 ◽  
Author(s):  
Hannes Ponstingl ◽  
Gottfried Otting
Keyword(s):  
Escherichia Coli ◽  
Secondary Structure ◽  
Nmr Assignments

Sequence-specific proton NMR assignments and secondary structure in solution of Escherichia coli trp repressor

Biochemistry ◽  
1990 ◽  
Vol 29 (27) ◽  
pp. 6332-6341 ◽  
Author(s):  
Cheryl H. Arrowsmith ◽  
R. Pachter ◽  
R. B. Altman ◽  
S. B. Iyer ◽  
O. Jardetzky
Keyword(s):  
Escherichia Coli ◽  
Secondary Structure ◽  
Nmr Assignments ◽  
Proton Nmr ◽  
Trp Repressor ◽  
Structure In Solution

Filarial DNA and its interaction with polyamines and antifilarial drugs

1997 ◽  
Vol 71 (4) ◽  
pp. 325-332 ◽  
Author(s):  
U. Pandya ◽  
J.K. Saxena ◽  
O.P. Shukla
Keyword(s):  
Escherichia Coli ◽  
Secondary Structure ◽  
Melting Temperature ◽  
Base Composition ◽  
Micrococcus Luteus ◽  
Gc Content ◽  
Setaria Cervi ◽  
Calf Thymus ◽  
Absorption Studies ◽  
Z Dna

AbstractThe interaction of DNA from filarial parasite Setaria cervi with polyamines was monitored by melting temperature (Tm) profile, condensation and B to Z DNA transition and compared with DNA of Escherichia coli, Micrococcus luteus and calf thymus having different GC content. Polyamines, viz. spermine and spermidine, stabilized the secondary structure of all DNAs as indicated by increase in Tm value. UV absorption studies indicated B to Z DNA transition in the presence of polyamines. The amount of polyamines required for B to Z transition was dependent upon base composition of DNA and charge of the polyamine. Filarial DNA (AT rich) required six times higher concentration of spermine as compared to GC rich DNA for B to Z DNA transition. Spermidine was not effective in causing transition of S. cervi DNA even at Spd:DNA-P ratio of 20. The antifilarial compound suramin significantly decreased melting temperature of filarial DNA as compared to GC rich DNAs of other parasites. Suramin adversely affected condensation and B to Z DNA transition of various DNAs but prior addition of polyamines protected the DNAs from the destabilizing effect of suramin.

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