RENIN GENE EXPRESSION IN VARIOUS TISSUES DETERMINED BY SINGLE-STEP POLYMERASE CHAIN REACTION

1991 ◽  
Vol 18 (5) ◽  
pp. 357-362 ◽  
Author(s):  
Yi-kun Lou ◽  
D. Lynne Smith ◽  
Bruce G. Robinson ◽  
Brian J. Morris
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Single Step ◽  
Chain Reaction ◽  
Renin Gene ◽  
Polymerase Chain ◽  
Step Polymerase Chain Reaction

scholarly journals Single step polymerase chain reaction (PCR) for the diagnosis of the Leishmania (Viannia) subgenus

2004 ◽  
Vol 46 (6) ◽  
pp. 335-338 ◽  
Author(s):  
Byanca Regina Paiva ◽  
Luciana Neves Passos ◽  
Aloisio Falqueto ◽  
Rosely dos S. Malafronte ◽  
Heitor Franco de Andrade Jr.
Keyword(s):  
Polymerase Chain Reaction ◽  
Diagnostic Tool ◽  
Repetitive Sequences ◽  
Etiologic Agent ◽  
Single Step ◽  
Chain Reaction ◽  
Parasitological Diagnosis ◽  
Mucosal Involvement ◽  
Polymerase Chain ◽  
Step Polymerase Chain Reaction

In Brazil, the main etiologic agent of Leishmaniasis that frequently presents with mucosal involvement belongs to the Viannia subgenus. The therapeutic conduct in this disease depends on the parasitological diagnosis, and classical methods are restricted in identifying the agent. In this paper we describe a polymerase chain reaction (PCR), which uses primers designed from mini-exons repetitive sequences. The PCR amplifies a 177bp fragment that can distinguish (Viannia) from (Leishmania) subgenus. This test could be a useful diagnostic tool.

Differential display competitive polymerase chain reaction: An optimal tool for assaying gene expression

1999 ◽  
Vol 20 (2) ◽  
pp. 230 ◽  
Author(s):  
Marianne Jorgensen ◽  
Maja Bévort ◽  
Thuri S. Kledal ◽  
Brian V. Hansen ◽  
Marlene Dalgaard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Differential Display ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain
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