Organism diversity between women with and without bacterial vaginosis as determined by polymerase chain reaction denaturing gradient gel electrophoresis and 16S rRNA gene sequence

2011 ◽  
Vol 37 (10) ◽  
pp. 1438-1446 ◽  
Author(s):  
Yutao Diao ◽  
Xueqiang Fang ◽  
Qing Xia ◽  
Shouqiang Chen ◽  
Hao Li ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
16S Rrna ◽  
Gel Electrophoresis ◽  
Gene Sequence ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Chain Reaction ◽  
Gradient Gel Electrophoresis ◽  
Polymerase Chain

Temperature Gradient Gel Electrophoresis of the Amplified Product of a Small 16S rRNA Gene Fragment for the Identification of Listeria Species Isolated from Food

2000 ◽  
Vol 63 (5) ◽  
pp. 659-661 ◽  
Author(s):  
MARISA MANZANO ◽  
LUCA COCOLIN ◽  
CARLO CANTONI ◽  
GIUSEPPE COMI
Keyword(s):  
Polymerase Chain Reaction ◽  
Temperature Gradient ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gel Electrophoresis ◽  
Rrna Gene ◽  
Chain Reaction ◽  
Gradient Gel Electrophoresis ◽  
Polymerase Chain ◽  
Temperature Gradient Gel Electrophoresis

The development of a rapid method for the identification of Listeria spp. is described. It is based on the polymerase chain reaction amplification of a small fragment from the 16S rRNA gene followed by temperature gradient gel electrophoresis. Forty-five strains of Listeria spp. (Listeria monocytogenes, Listeria innocua, Listeria ivanovii, Listeria seeligeri, and Listeria welshimeri) were used for the optimization of the protocol. No differences were observed between the results of the identification of the strains tested using traditional methods and those obtained by polymerase chain reaction–temperature gradient gel electrophoresis analysis.

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