M-cadherin Distribution in the Mouse Adult Neuromuscular System Suggests a Role in Muscle Innervation

The Drosophila larval neuromuscular system provides an ideal context in which to study synaptic partner choice, because it contains a small number of pre- and postsynaptic cells connected in an invariant pattern. The discovery of interactions between two subfamilies of IgSF cell surface proteins, the Dprs and the DIPs, provided new candidates for cellular labels controlling synaptic specificity. Here we show that DIP-α is expressed by two identified motor neurons, while its binding partner Dpr10 is expressed by postsynaptic muscle targets. Removal of either DIP-α or Dpr10 results in loss of specific axonal branches and NMJs formed by one motor neuron, MNISN-1s, while other branches of the MNISN-1s axon develop normally. The temporal and spatial expression pattern of dpr10 correlates with muscle innervation by MNISN-1s during embryonic development. We propose a model whereby DIP-α and Dpr10 on opposing synaptic partners interact with each other to generate proper motor neuron connectivity.

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Transsynaptic interactions between IgSF proteins DIP-α and Dpr10 are required for motor neuron targeting specificity in Drosophila

10.1101/424416 ◽

2018 ◽

Cited By ~ 2

Author(s):

James Ashley ◽

Violet Sorrentino ◽

Sonal Nagarkar-Jaiswal ◽

Liming Tan ◽

Shuwa Xu ◽

...

Keyword(s):

Motor Neuron ◽

Motor Neurons ◽

Surface Proteins ◽

Partner Choice ◽

Neuromuscular System ◽

Synaptic Specificity ◽

Binding Partner ◽

Spatial Expression Pattern ◽

Muscle Innervation ◽

Temporal And Spatial

ABSTRACTThe Drosophila larval neuromuscular system provides an ideal context in which to study synaptic partner choice, because it contains a small number of pre- and postsynaptic cells connected in an invariant pattern. The discovery of interactions between two subfamilies of IgSF cell surface proteins, the Dprs and the DIPs, provided new candidates for cellular labels controlling synaptic specificity. Here we show that DIP-α is expressed by two identified motor neurons, while its binding partner Dpr10 is expressed by postsynaptic muscle targets. Removal of either DIP-α or Dpr10 results in loss of specific axonal branches and NMJs formed by one motor neuron, MNISN-1s, while other branches of the MNISN-1s axon develop normally. The temporal and spatial expression pattern of dpr10 correlates with muscle innervation by MNISN-1s during embryonic development. We propose a model whereby DIP-α and Dpr10 on opposing synaptic partners interact with each other to generate proper motor neuron connectivity.

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Functional limb muscle innervation prior to cholinergic transmitter specification during early metamorphosis in Xenopus

eLife ◽

10.7554/elife.30693 ◽

2018 ◽

Vol 7 ◽

Cited By ~ 5

Author(s):

Francois M Lambert ◽

Laura Cardoit ◽

Elric Courty ◽

Marion Bougerol ◽

Muriel Thoby-Brisson ◽

...

Keyword(s):

Nicotinic Receptors ◽

System Development ◽

Neuromuscular System ◽

Spinal Neurons ◽

Neuromuscular Synapses ◽

Hindlimb Muscles ◽

Peripheral Muscle ◽

Glutamate Antagonist ◽

Muscle Innervation ◽

Functional Limb

In vertebrates, functional motoneurons are defined as differentiated neurons that are connected to a central premotor network and activate peripheral muscle using acetylcholine. Generally, motoneurons and muscles develop simultaneously during embryogenesis. However, during Xenopus metamorphosis, developing limb motoneurons must reach their target muscles through the already established larval cholinergic axial neuromuscular system. Here, we demonstrate that at metamorphosis onset, spinal neurons retrogradely labeled from the emerging hindlimbs initially express neither choline acetyltransferase nor vesicular acetylcholine transporter. Nevertheless, they are positive for the motoneuronal transcription factor Islet1/2 and exhibit intrinsic and axial locomotor-driven electrophysiological activity. Moreover, the early appendicular motoneurons activate developing limb muscles via nicotinic antagonist-resistant, glutamate antagonist-sensitive, neuromuscular synapses. Coincidently, the hindlimb muscles transiently express glutamate, but not nicotinic receptors. Subsequently, both pre- and postsynaptic neuromuscular partners switch definitively to typical cholinergic transmitter signaling. Thus, our results demonstrate a novel context-dependent re-specification of neurotransmitter phenotype during neuromuscular system development.

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Functional limb muscle innervation prior to cholinergic transmitter specification during early metamorphosis in Xenopus

10.1101/173328 ◽

2017 ◽

Author(s):

F. M. Lambert ◽

L. Cardoit ◽

E. Courty ◽

M. Bougerol ◽

M. Thoby-Brisson ◽

...

Keyword(s):

Nicotinic Receptors ◽

System Development ◽

Neuromuscular System ◽

Spinal Neurons ◽

Neuromuscular Synapses ◽

Hindlimb Muscles ◽

Peripheral Muscle ◽

Glutamate Antagonist ◽

Muscle Innervation ◽

Functional Limb

ABSTRACTIn vertebrates, functional motoneurons are defined as differentiated neurons that are connected to a central premotor network and activate peripheral muscle using acetylcholine. Generally, motoneurons and muscles develop simultaneously during embryogenesis. However, during Xenopus metamorphosis, developing limb motoneurons must reach their target muscles through the already established larval cholinergic axial neuromuscular system. Here, we demonstrate that at metamorphosis onset, spinal neurons retrogradely labeled from the emerging hindlimbs initially express neither choline acetyltransferase nor vesicular acetylcholine transporter. Nevertheless, they are positive for the motoneuronal transcription factor Islet1/2 and exhibit intrinsic and axial locomotor-driven electrophysiological activity. Moreover, the early appendicular motoneurons activate developing limb muscles via nicotinic antagonist-resistant, glutamate antagonist-sensitive, neuromuscular synapses. Coincidently, the hindlimb muscles transiently express glutamate, but not nicotinic receptors. Subsequently, both pre- and postsynaptic neuromuscular partners switch definitively to typical cholinergic transmitter signaling. Thus, our results demonstrate a novel context-dependent re-specification of neurotransmitter phenotype during neuromuscular system development.

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Ultrastructure of neuromuscular relationships in the canine heartworm (dirofilaria immitis)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100143018 ◽

1986 ◽

Vol 44 ◽

pp. 278-279

Author(s):

W. L. Steffens ◽

Nancy B. Roberts ◽

J. M. Bowen

Keyword(s):

Dirofilaria Immitis ◽

Domestic Dogs ◽

Structural Description ◽

Pharmacological Effects ◽

Free Living ◽

Canine Heartworm ◽

The World ◽

Synaptic Region ◽

Muscle Innervation ◽

Insight Into

The canine heartworm is a common and serious nematode parasite of domestic dogs in many parts of the world. Although nematode neuroanatomy is fairly well documented, the emphasis has been on sensory anatomy and primarily in free-living soil species and ascarids. Lee and Miller reported on the muscular anatomy in the heartworm, but provided little insight into the peripheral nervous system or myoneural relationships. The classical fine-structural description of nematode muscle innervation is Rosenbluth's earlier work in Ascaris. Since the pharmacological effects of some nematacides currently being developed are neuromuscular in nature, a better understanding of heartworm myoneural anatomy, particularly in reference to the synaptic region is warranted.

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Effect of caffeine on the neuromuscular system in rats: an immunohistochemical study

Planta Medica ◽

10.1055/s-0029-1234740 ◽

2009 ◽

Vol 75 (09) ◽

Author(s):

P Lo Cascio ◽

ER Lauriano ◽

A Leuzzi ◽

L Campolo ◽

M Calò ◽

...

Keyword(s):

Immunohistochemical Study ◽

Neuromuscular System

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Asociación entre hábitos parafuncionales de la cavidad bucal y los transtornos temporomandibulares en adolescentes

REVISTA ODONTOLOGÍA PEDIÁTRICA ◽

10.33738/spo.v10i2.167 ◽

2018 ◽

Vol 10 (2) ◽

Author(s):

Gabriel Muñoz Quintana

Keyword(s):

Oral Cavity ◽

Temporomandibular Joint ◽

Temporomandibular Disorders ◽

Temporomandibular Disorder ◽

Neuromuscular System ◽

Jaw Movements ◽

Nail Biting ◽

Palabras Clave ◽

Masticatory System ◽

Objective Being

La musculatura del sistema masticatorio y la articulación temporomandibular (ATM) están protegidos por reflejos nerviosos básicos y sistema neuromuscular a través de la coordinación de fuerzas musculares, todo lo que produce sobrecarga muscular repetitiva como los hábitos parafuncionales (HPF) pueden ocasionar trastornos temporomandibulares (TTM)1. Los HPF se caracterizan por movimientos anormales a la función mandibular normal sin objetivo funcional, al estar alterados constituyen una fuente productora de fuerzas traumáticas caracterizadas por dirección anormal, intensidad excesiva y repetición frecuente y duradera (Rolando Castillo Hernández, 2001)4. El objetivo del estudio fue identificar la asociación entre la presencia de hábitos parafuncionales de la cavidad bucal y los TTM en adolescentes de la ciudad de Puebla. Estudio observacional descriptivo. Se incluyeron 258 adolescentes, 132 (51.2%) mujeres y 126 (48.8%) hombres, con una edad promedio de 12.5±.73 y quienes fueron diagnosticados con los CDI/TTM y los HPF fueron auto-reportados por los pacientes. Se encontró una prevalencia de los TTM del 39.9% y una prevalencia de HPF del 86%. Los HPF más frecuentemente reportados fueron la succión labial y la onicofagia. Se encontró una asociación significativa (x2=7.31, p=0.007) entre los hábitos parafuncionales y los TTM en adolescentes. Palabras clave: Trastornos temporomandibulares, hábitos parafuncionales, adolescentes, articulación temporomandibular. Abstract The muscles of the masticatory system and temporomandibular joint (TMJ) are protected by basic nerve reflex and neuromuscular system through the coordination of muscle forces, all that repetitive muscle overload occurs as habit parafunctional (HPF) can cause temporomandibular disorder TMD)1. The characteristics of HPF are abnormal jaw movements without a functional objective. Being the jaw movements altered, they constitute a source of traumatic forces with an abnormal direction, excessive intensity and long-lasting and frequent duration. (Rolando Hernandez Castillo 2001)4. Objective: was to identify the association between the presences of parafunctional habits of the oral cavity and TMD in adolescents in the Puebla city in Mexico. Material and methods: Is a observational study, we included 258 adolescents 132 (51%) females and 126 (48.8%) were men, mean age 12.5±.73 and who were diagnosed with CDI/TTM and HPF were self- reported by patients. Results: The prevalence of TMD was 39.9% and a prevalence of 86% HPF. The most frequently reported HPF were lip sucking and nail biting. We found a significant association (x2= 7.31, p = 0,007) between HPF and TMD in adolescents. Key words: Parafunctional habits of oral cavity, temporomandibular disorders, temporomandibular joint. (Odontol Pediatr 2011;10(2): 90-94).

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