scholarly journals Mycorrhizal synthesis in vitro between Glomus spp. and artificial seeds of alfalfa

1989 ◽  
Vol 113 (4) ◽  
pp. 545-548 ◽  
Author(s):  
D. G. STRULLU ◽  
C. ROMAND ◽  
P. CALLAC ◽  
E. TEOULE ◽  
Y. DEMARLY
Keyword(s):  
Artificial Seeds ◽  
Mycorrhizal Synthesis ◽  
Glomus Spp

scholarly journals Micropropagation of Cymbidium aloifolium (L.) SW., A Medicinal Orchid by Artificial Seeds Technology

2015 ◽  
Vol 28 ◽  
pp. 42-48 ◽  
Author(s):  
Shreeti Pradhan ◽  
Babu Lal Tiruwa ◽  
Bijay R Subedee ◽  
Bijaya Pant
Keyword(s):  
Basal Medium ◽  
Mass Scale ◽  
Scale Production ◽  
Ms Medium ◽  
Artificial Seeds ◽  
Seed Technology ◽  
Artificial Seed ◽  
Cymbidium Aloifolium ◽  
Medicinal Orchid

Artificial seed technology is a rapidly growing area of research in plant cell and tissue culture. Application of this technology opens an alternative route for mass scale production, efficient delivery of cloned plantlets and fulfils the increasing demand of local growers. An attempt was made to produce artificial seeds and their subsequent regeneration of a highly valuable medicinal orchid of Nepal i.e. Cymbidium aloifolium. Artificial seeds were obtained through encapsulation of protocorms in calcium alginate beads. Protocorms were encapsulated by using 3% sodium alginate and 0.2 M calcium chloride solution. Murashige and Skoog (MS) medium (1962) was used as the basal medium for in vitro germination and seedling development of artificial seed. In Cymbidium aloifolium, 20-25 days old in vitro grown protocorms were used for production of artificial seeds. Artificial seeds were inoculated on two different culture conditions of MS medium i.e. MS solid & MS liquid with four different treatments i.e. strength of 1.0, ½, ¼ and MS media supplemented with plant growth regulators viz. BAP (0.5 mg/l) and NAA (0.5 mg/l). Highest percentage of germination (100%) and plantlet conversion was found on hormone free full strength (1.0 MS) of MS liquid medium after 13-14 weeks of culture. Plantlets regenerated from artificial seeds with well developed shoot and root systems were successfully acclimatized in potting mixture of cocopeat, litter and sphagnum moss in a ratio 2:1:1.J. Nat. Hist. Mus. Vol. 28, 2014: 42-48

scholarly journals Mycorrhizal synthesis between Pisolithus arhizus and adult clones of Arbutus unedo in vitro and in nursery

2013 ◽  
Vol 24 (4) ◽  
pp. 659-670 ◽  
Author(s):  
Filomena Gomes ◽  
Helena Machado ◽  
Esteban San Martin ◽  
A. Portugal ◽  
Jorge M. Canhoto
Keyword(s):  
Arbutus Unedo ◽  
Mycorrhizal Synthesis ◽  
Pisolithus Arhizus

scholarly journals Effect of Quercetin on Mycorrhizal Synthesis between Tuberborchii and Arbutusunedo L. In Vitro Plants

2021 ◽  
Vol 12 (1) ◽  
pp. 69-81
Author(s):  
Bárbara Gomes ◽  
Fábio Castro ◽  
Rita Santos ◽  
Patrícia Figueiredo ◽  
Márcia Silva ◽  
...  
Keyword(s):  
Forest Fires ◽  
Culture Medium ◽  
Culture Media ◽  
Fruit Production ◽  
Regeneration Ability ◽  
Specific Primers ◽  
Tuber Borchii ◽  
Mycorrhizal Synthesis ◽  
Hydric Stress

Arbutus unedo L. is a Mediterranean species used for fruit production; it is tolerant to drought and shows regeneration ability following forest fires. Mycorrhizal plants with Tuber borchii add resilience and value. This study aims to test the effect of quercetin on mycorrhizal synthesis between T. borchii and A. unedo. Two genotypes selected for fruit production and hydric stress tolerance, were micropropagated for mycorrhizal synthesis, accomplished during ex vitro rooting in perlite, using lyophilized spores of T. borchii suspended in culture media with different quercetin levels (0–10 µM). Six months after inoculation, plants were transferred to pots and maintained in nursery. Ten and 12 months after inoculation, roots were morphological examined and molecularly characterized using ITS1-5.8SITS2 rDNA region and specific primers. Results showed that mycorrhizae establishment was dependent on studied factors (genotype, quercetin level, and culture medium) and their interaction (genotype X culture medium). Quercetin levels up to 2.0 µM favored mycorrhizae establishment and plant growth, although levels superior to 4 µM showed a toxic effect. Quercetin showed to be an efficient factor on inducing mycorrhiza thriving independent of the genotype. Morphological observations and molecular analysis confirmed the permanence of the fungus association 10 and 12 months after inoculation.

scholarly journals Impact of Encapsulation on Plantlet Regeneration from in vitro Grown Shoot tips of Citrus aurantifolia (Lime)

2021 ◽  
Vol 31 (1) ◽  
pp. 43-49
Author(s):  
Priyanka Sharma ◽  
Bidhan Roy
Keyword(s):  
Farmyard Manure ◽  
Synthetic Seeds ◽  
Shoot Tips ◽  
Artificial Seeds ◽  
Sterile Soil ◽  
Citrus Aurantifolia ◽  
Diverse Species ◽  
In Vitro Micropropagation ◽  
Regenerated Plant

In order to conserve diverse species of citrus, an experiment on in vitro micropropagation and production of synthetic seeds from in vitro regenerated plant propagules of the species; Citrus aurantifolia (Lime) was carried out in which shoot tips were found to be suitable for excapsulation of artificial seeds. Highest rate of germination was obtained from the shoot tips when MS was supplemented with 1 mg/l BAP. Beaded shoot tips produced maximum germination (81.43%). Germinated synthetic seeds with well established roots and shoots were were taken out from the culture bottles and transferred in plastic cups containing a mixture of sterile soil: sand and farmyard manure at a ratio of 1:1:1. Seedlings were further shifted in earthen pots and kept in a partial shed net house for 7 days. Those seedlings were finally transferred under the field conditions for acclimatization. Plant Tissue Cult. & Biotech. 31(1): 43-49, 2021 (June)

In vitro regeneration of Aristolochia tagala and production of artificial seeds

2013 ◽  
Vol 57 (2) ◽  
pp. 210-218 ◽  
Author(s):  
M. Remya ◽  
V. Bai ◽  
V. N. Mutharaian
Keyword(s):  
In Vitro Regeneration ◽  
Artificial Seeds
Sign in / Sign up

Export Citation Format

Share Document