BIODIVERSITY OF FRUIT-BEARING FOREST SPECIES IN THE PROTECTED LANDSCAPE "KONJUH"

UDK: 634.2:574.4]:502(497.6 “Konjuh“) The area of the protected landscape "Konjuh" is distinguished by quality forest ecosystems, featuring diverse fruit-bearing forest species, which contributes to the conservation of biodiversity of the protected landscape "Konjuh". The aim of the paper is to assess the state of biodiversity of fruit-bearing species in the forest ecosystems of the study area, with the emphasis on determining the genus richness as a basis for conservation (in/ex situ), breeding and economic use. During the vegetation season in 2015 and 2016, several relevés (phytosociological plot) were made in the Protected Landscape "Konjuh". The results of the research indicate the high level of variability for most of the species according to characteristics of biology, pomology, and ecology. The fruit trees, like the noble hardwood, are wild cherry, (Prunus avium L.), wild pear (Pyrus communis L.), wild apples (Malus sylvestris Mill.), Breccia (Torminalis clusii M. Roem.), Sorbus aria L. et all, Wild cherry, Vrapcarka (Prunus avium L.) is the most famous forest fruit grower, occurs as a single tree or in smaller groups (Noćajević, 2009). The benefits of fruit-bearing forest species are emphasized in the bloom and the fruiting time, when "decorated" forest provide a rich bee pasture and feeding nutriment for forest fauna (Orešković et al. 2006). Fruit-bearing Forest species are significant as the genera of the varieties and as a basis for the grafting for high-grade varieties. Also, they are important for humans from the aspect of ecology, nutrition, dietotherapy, pharmacology and bioenergy balance.

Antioxidant Activity of Some Fluids Extracts of Indigenous Wild Cherry Fruits

The paper presents un study regarding the comparative total antioxidant capacity of some fluids extracts of the soluble lipid compounds of Cerasus avium (L.) Moench. syn. Prunus avium (L.) var. sylvestris Ser fruits, popular named wild cherry, species undertaken aiming at exploiting the nutrient profiles and promoting the further development of these indigen phytopharmaceutical resources. For analysis, the photo-chemiluminescence method, ACL procedure, Analytik Jena AG, by comparing with the standard Trolox® solution, was used. Wild cherry fruits fluid extracts analyzed emphasize an increased total antioxidative capacity which may be attributed to the high level content of polyphenolic compounds and minerals content which sustains the possibility to use this valuable vegetal product for nutraceutical or comeceutical preparations.

Genetic differentiation in functional traits among wild cherry (Prunus avium L.) half-sib lines

Understanding intra-specific variation in leaf functional traits is one of the key requirements for the evaluation of species adaptive capacity to ongoing climate change, as well as for designing long-term breeding and conservation strategies. Hence, data of 19 functional traits describing plant physiology, antioxidant properties, anatomy and morphology were determined on 1-year-old seedlings of wild cherry (Prunus avium L.) half-sib lines. The variability within and among half-sib lines, as well as the estimation of multi-trait association, were examined using analysis of variance (ANOVA) followed by Tukey's honestly significant difference test and multivariate analyses: principal component analysis (PCA), canonical discriminant analysis (CDA) and stepwise discriminant analysis (SDA). Pearson’s correlation coefficient was used to evaluate linear correlation between the study parameters. The results of the ANOVA showed the presence of statistically significant differences (P < 0.01) among half-sib lines for all study traits. The differences within half-sib lines, observed through the contribution of the examined sources of variation to the total variance (%), had higher impact on total variation in the majority of the examined traits. Pearson’s correlation analysis and PCA showed strong relationships between gas exchange in plants and leaf size and stomatal density, as well as between leaf biomass accumulation, intercellular CO2 concentration and parameters related to antioxidant capacity of plants. Likewise, the results of SDA indicate that transpiration and stomatal conductance contributed to the largest extent, to the discrimination of the wild cherry half-sib lines. In addition, PCA and CDA showed separation of the wild cherry half-sib lines along the first principal component and first canonical variable with regards to humidity of their original sites. Multiple adaptive differences between the wild cherry half-sib lines indicate high potential of the species to adapt rapidly to climate change. The existence of substantial genetic variability among the wild cherry half-sib lines highlights their potential as genetic resources for reforestation purposes and breeding programmes.

HPLC-DAD-APCI-MS as a Tool for Carotenoid Assessment of Wild and Cultivated Cherry Tomatoes

Carotenoids are naturally occurring fat-soluble pigments found in many organisms. Because of their extensively conjugated carbon–carbon double bond system, carotenoids are potent antioxidants. Although the most abundant carotenoid and best singlet oxygen quencher found in red tomatoes is lycopene, carotenoid profiles may vary between genotypes. The objective of this work was to perform carotenoid profile indentification using HPLC-DAD-APCI-MS in ten wild cherry tomato accessions and one cultivated tomato. A mixture of hexane/acetone/ethanol (50:25:25) and 0.1% BHT was used for carotenoid extraction. For separation, a C30 column at 30 °C with a gradient consisting of methanol, methyl-tert-butyl ether, and water was used for their analysis. Ten major carotenoids were quantified within cherry tomato samples. All accessions present different profiles and quantities of carotenoids. Wild red tomatoes had more lycopene content that commercial tomato, whereas yellow tomatoes present no lycopene. From a functional viewpoint, higher concentrations of carotenoids that could play an antioxidant activity were measured from accessions IAC401, IAC426, LA1480, IAC391, and LA2692. This trait means that these germplasms may be targets for commercial activities. To the best of our knowledge, this is the first time that HPLC-DAD-APCI-MS has been used to analyze these accessions of wild cherry tomatoes that are both functionally promising and suitable for projects with social implementation at a local scale.

The study of parent-regeneration relationships for wild cherry (Prunus avium L.) in Hyrcanian forests

The regeneration of forest trees is affected by the presence, dispersion pattern and specifications of parent trees. These effects were investigated and modelled in hardwood forests in the north of Iran. To do so, at first, fifty plots, each with a total area of 0.1 ha, were identified. Four subplots with a radius of two meters were then stabilized in each plot. The height and the root collar diameter of all the cherry seedlings were measured. Likewise, the diameter, basal area, and distance from the plot centre were appraised in the parent trees. The correlation and regression analyses were performed to evaluate the effects and to construct the models, respectively. The results showed that there was a positive and significant relationship between the presence, abundance, mean diameter, and total basal area of parent trees and the presence and abundance of seedlings. The logistic regression showed that the models based on the mean diameter of parent trees and abundance of parent trees could significantly predict the presence of regeneration. Only in 10% of the sample plots was the regeneration recorded without the presence of a parent tree. Due to the small number of established seedlings, while maintaining the existing parent trees, especially large trees, it is necessary to consider the proper management methods to promote and protect their regeneration. We recommend completing the natural regeneration of wild cherry by artificial regeneration and creating well-dimensioned canopy gaps.

Modelling and Comparing Shading Effects of 3D Tree Structures with Virtual Leaves

Reduced solar radiation brought about by trees on agricultural land can both positively and negatively affect crop growth. For a better understanding of this issue, we aim for an improved simulation of the shade cast by trees in agroforestry systems and a precise estimation of insolation reduction. We present a leaf creation algorithm to generate realistic leaves to be placed upon quantitative structure models (QSMs) of real trees. Further, we couple it with an enhanced approach of a 3D model capable of quantifying shading effects of a tree, at a high temporal and spatial resolution. Hence, 3D data derived from wild cherry trees (Prunus avium L.) generated by terrestrial laser scanner technology formed a basis for the tree reconstruction, and served as leaf-off mode. Two leaf-on modes were simulated: realistic leaves, fed with leaf data from wild cherry trees; and ellipsoidal leaves, having ellipsoids as leaf-replacement. For comparison, we assessed the shading effects using hemispherical photography as an alternative method. Results showed that insolation reduction was higher using realistic leaves, and that the shaded area was greater in size than with the ellipsoidal leaves or leaf-off conditions. All shading effects were similarly distributed on the ground, with the exception of those derived through hemispherical photography, which were greater in size, but with less insolation reduction than realistic leaves. The main achievements of this study are: the enhancement of the leaf-on mode for QSMs with realistic leaves, the updates of the shadow model, and the comparison of shading effects. We provide evidence that the inclusion of realistic leaves with precise 3D data might be fundamental to accurately model the shading effects of trees.