CARBOHYDRATE AND ENERGY METABOLISM IN PERINATAL RAT BRAIN: RELATION TO SURVIVAL IN ANOXIA

1975 ◽  
Vol 24 (2) ◽  
pp. 271-276 ◽  
Author(s):  
T. E. Duffy ◽  
S. J. Kohle ◽  
R. C. Vannucci
Keyword(s):  
Energy Metabolism ◽  
Rat Brain

scholarly journals Comparison of the Effects of Cyclosporin a on the Metabolism of Perfused Rat Brain Slices during Normoxia and Hypoxia

2002 ◽  
Vol 22 (3) ◽  
pp. 342-352 ◽  
Author(s):  
Natalie Serkova ◽  
Paul Donohoe ◽  
Sven Gottschalk ◽  
Carsten Hainz ◽  
Claus U. Niemann ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Cyclosporin A ◽  
Rat Brain ◽  
Ex Vivo ◽  
Brain Slices ◽  
High Energy ◽  
Metabolic Effects ◽  
Resonance Spectroscopy ◽  
Buffer Solution ◽  
Mitochondrial Energy Metabolism

The authors evaluated and compared the metabolic effects of cyclosporin A in the rat brain during normoxia and hypoxia/reperfusion. Ex vivo31P magnetic resonance spectroscopy experiments based on perfused rat brain slices showed that under normoxic conditions, 500 μg/L cyclosporin A significantly reduced mitochondrial energy metabolism (nucleotide triphosphate, 83 ± 9% of controls; phosphocreatine, 69 ± 9%) by inhibition of the Krebs cycle (glutamate, 77 ± 5%) and oxidative phosphorylation (NAD+, 65 ± 14%) associated with an increased generation of reactive oxygen species (285 ± 78% of control). However, the same cyclosporin A concentration (500 μg/L) was found to be the most efficient concentration to inhibit the hypoxia-induced mitochondrial release of Ca2+ in primary rat hippocampal cells with cytosolic Ca2+ concentrations not significantly different from normoxic controls. Addition of 500 μg/L cyclosporin A to the perfusion medium protected high-energy phosphate metabolism (nucleotide triphosphate, 11 ± 15% of control vs. 35 ± 9% with 500 μg/L cyclosporin A) and the intracellular pH (6.2 ± 0.1 control vs. 6.6 ± 0.1 with cyclosporin A) in rat brain slices during 30 minutes of hypoxia. Results indicate that cyclosporin A simultaneously decreases and protects cell glucose and energy metabolism. Whether the overall effect was a reduction or protection of cell energy metabolism depended on the concentrations of both oxygen and cyclosporin A in the buffer solution.

Glutaric acid moderately compromises energy metabolism in rat brain

2005 ◽  
Vol 23 (8) ◽  
pp. 687-693 ◽  
Author(s):  
Gustavo da C. Ferreira ◽  
Carolina M. Viegas ◽  
Patrícia F. Schuck ◽  
Alexandra Latini ◽  
Carlos S. Dutra-Filho ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Rat Brain ◽  
Glutaric Acid

scholarly journals Effect of Astrocytic Energy Metabolism Depressant on 14C-Acetate Uptake in Intact Rat Brain

2004 ◽  
Vol 24 (2) ◽  
pp. 188-190 ◽  
Author(s):  
Rie Hosoi ◽  
Maki Okada ◽  
Jun Hatazawa ◽  
Antony Gee ◽  
Osamu Inoue
Keyword(s):  
Blood Flow ◽  
Energy Metabolism ◽  
Rat Brain ◽  
Regional Blood Flow ◽  
Regional Distribution ◽  
Acetate Uptake ◽  
Minute Period ◽  
Intact Brain ◽  
The Right

Fluorocitrate, a selective astrocytic toxin, was microinjected into the right striatum of rat brain, and the regional distribution of 14C-acetate was measured using autoradiography. A significant reduction (more than 80%) in 14C-acetate uptake over a 5-minute period was observed in the right striatum, compared with that in the left striatum (saline infused), 4 hours after fluorocitrate (1 nmol/μL) infusion. This effect was transient, and 14C-acetate uptake had almost returned to normal at 24 hours after the fluorocitrate infusion. In contrast, the regional blood flow in the striatum, as determined using 14C-iodoamphetamine, was significantly increased by the fluorocitrate infusion. The present observations indicate that 14C-acetate uptake might be a useful characteristic for examining astrocytic energy metabolism in the intact brain.

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