In vitro effect of manganese chloride exposure on energy metabolism and oxidative damage of mitochondria isolated from rat brain

2008 ◽  
Vol 26 (2) ◽  
pp. 232-236 ◽  
Author(s):  
Fanglin Zhang ◽  
Zhaofa Xu ◽  
Jian Gao ◽  
Bin Xu ◽  
Yu Deng
Keyword(s):  
Energy Metabolism ◽  
Oxidative Damage ◽  
Rat Brain ◽  
Manganese Chloride ◽  
Vitro Effect

In Vitro Effect of Lead on Na+, K+-ATPase Activity in Different Regions of Adult Rat Brain

2003 ◽  
Vol 26 (2) ◽  
pp. 117-124 ◽  
Author(s):  
Prabhakara Rao Yallapragada ◽  
Jennifer Butler ◽  
Bokara Kiran Kumar ◽  
Bettaiya Rajanna
Keyword(s):  
Atpase Activity ◽  
Rat Brain ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Vitro Effect

scholarly journals Oxidative damage in mitochondrial fatty acids oxidation disorders patients and the in vitro effect of l-carnitine on DNA damage induced by the accumulated metabolites

2020 ◽  
Vol 679 ◽  
pp. 108206 ◽  
Author(s):  
Maira Silmara de Moraes ◽  
Gilian Guerreiro ◽  
Angela Sitta ◽  
Daniella de Moura Coelho ◽  
Vanusa Manfredini ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dna Damage ◽  
Oxidative Damage ◽  
Vitro Effect

The in vitro effect of l-prolyl-l-leucyl-glycineamide (MIF-1) on the guanylate cyclase system of a crude rat brain mitochondrial fraction

Neuropeptides ◽  
1981 ◽  
Vol 1 (5) ◽  
pp. 391-400 ◽  
Author(s):  
A.R. Gerber ◽  
K.S. Wood ◽  
A.O. Sartor ◽  
W.F. Wheeler ◽  
T.C. Gayle ◽  
...  
Keyword(s):  
Rat Brain ◽  
Guanylate Cyclase ◽  
Mitochondrial Fraction ◽  
Vitro Effect

In vitro effect of antipsychotics on brain energy metabolism parameters in the brain of rats

2013 ◽  
Vol 25 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Giselli Scaini ◽  
Natália Rochi ◽  
Meline O. S. Morais ◽  
Débora D. Maggi ◽  
Bruna T. De-Nês ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Respiratory Chain ◽  
Antipsychotic Drugs ◽  
Reaction Medium ◽  
Respiratory Chain Complexes ◽  
Posterior Cortex ◽  
Chain Complexes ◽  
Vitro Effect ◽  
The Brain

ObjectiveTypical and atypical antipsychotic drugs have been shown to have different clinical, biochemical and behavioural profiles. It is well described that impairment of metabolism, especially in the mitochondria, leads to oxidative stress and neuronal death and has been implicated in the pathogenesis of a number of diseases in the brain. In this context, we investigated the in vitro effect of antipsychotic drugs on energy metabolism parameters in the brain of rats.MethodsClozapine (0.1, 0.5 and 1.0 mg/ml), olanzapine (0.1, 0.5 and 1.0 mg/ml) and aripiprazole (0.05, 0.15 and 0.3 mg/ml) were suspended in buffer and added to the reaction medium containing rat tissue homogenates and the respiratory chain complexes, succinate dehydrogenase and creatine kinase (CK) activities were evaluated.ResultsOur results showed that olanzapine and aripriprazole increased the activities of respiratory chain complexes. On the other hand, complex IV activity was inhibited by clozapine, olanzapine and aripriprazole. CK activity was increased by clozapine at 0.5 and 1.0 mg/ml in prefrontal cortex, cerebellum, striatum, hippocampus and posterior cortex of rats. Moreover, olanzapine and aripiprazole did not affect CK activity.ConclusionIn this context, if the hypothesis that metabolism impairment is involved in the pathophysiology of neuropsychiatric disorders is correct and these results also occur in vivo, we suggest that olanzapine may reverse a possible diminution of metabolism.

Transformation of creatine kinase-BB isoenzyme in vitro: effect of carboxylic acids, thiols, pH, cations, and chelators.

1982 ◽  
Vol 28 (12) ◽  
pp. 2414-2417 ◽  
Author(s):  
J A Lott ◽  
J W Heinz
Keyword(s):  
Creatine Kinase ◽  
Rat Brain ◽  
Carboxylic Acids ◽  
Alkaline Ph ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Bb ◽  
Vitro Effect ◽  
Dialyzed Serum

Abstract Creatine kinase isoenzyme BB from rat brain was incubated with serum, dialyzed serum, or plasma, with added carboxylic acids, thiols, buffers, or cations. It was found to be very unstable at 37 degrees C under all these conditions, being transformed to CK-BB', a form that migrates like CK-MB on agarose electrophoretic plates. This transformation is enhanced at alkaline pH and, especially, by Zn2+. CK-BB' is quite stable, and probably results from binding of cations to CK-BB, because the transformation is prevented by EDTA and citrate.

Effects of In Vitro Hypoxia and Lowered pH on Potassium Fluxes and Energy Metabolism in Rat Brain Synaptosomes

1981 ◽  
Vol 36 (1) ◽  
pp. 116-123 ◽  
Author(s):  
Anna Pastuszko ◽  
David F. Wilson ◽  
Maria Erecińska ◽  
Ian A. Silver
Keyword(s):  
Energy Metabolism ◽  
Rat Brain ◽  
Rat Brain Synaptosomes ◽  
Brain Synaptosomes
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