Effect of Bicuculline-Induced Status Epilepticus on Prostaglandins and Hydroxyeicosatetraenoic Acids in Rat Brain Subcellular Fractions

1987 ◽  
Vol 48 (6) ◽  
pp. 1768-1778 ◽  
Author(s):  
Dale L. Birkle ◽  
Nicolas G. Bazan
Keyword(s):  
Status Epilepticus ◽  
Rat Brain ◽  
Subcellular Fractions ◽  
Hydroxyeicosatetraenoic Acids

scholarly journals [3H]2-(4-Phenylpiperidino)cyclohexanol (AH5183) binding to synaptosomes and subcellular fractions obtained from rat brain

1993 ◽  
Vol 157 (1) ◽  
pp. 72-74 ◽  
Author(s):  
Takeshi Suzuki ◽  
Yuko Kashima ◽  
Kazuko Fujimoto ◽  
Koichiro Kawashima
Keyword(s):  
Rat Brain ◽  
Subcellular Fractions

scholarly journals Binding of 1,1,1-trichloro-2,2-di-p-chlorophenylethane (DDT) with subcellular fractions of rat brain

1969 ◽  
Vol 114 (1) ◽  
pp. 135-139 ◽  
Author(s):  
Hans Brunnert ◽  
Fumio Matsumura
Keyword(s):  
Rat Brain ◽  
Nerve Ending ◽  
Soluble Proteins ◽  
Subcellular Fractions ◽  
Nerve Endings ◽  
Sephadex Column ◽  
Column Method ◽  
Synaptic Complexes ◽  
Soluble Components

DDT (1,1,1-trichloro-2,2-di-p-chlorophenylethane) and DDE (1,1-dichloro-2,2-di-p-chlorophenylethylene, a non-insecticidal analogue of DDT) were found to bind with various nerve components of rat brain. The amount of DDT binding exceeded that of DDE only in the fraction containing mainly the nerve endings. Among various components in the nerve-ending fraction, a subfraction containing mainly the pre- and post-synaptic complexes had the highest affinity for DDT in comparison with that for DDE. By using an ‘acetone-powdering’ technique on brain preparations, the Sephadex-column method was shown to provide reliable results for studies on the binding of DDT or DDE with soluble proteins in the nerve tissues. From these results it was concluded that DDE had a higher affinity for soluble components of the rat brain than did DDT.

LOCALIZATION AND CHARACTERIZATION OF CHOLINESTERASE IN SUBCELLULAR FRACTIONS OF RAT BRAIN AND BEEF PITUITARY

1961 ◽  
Vol 39 (9) ◽  
pp. 1335-1345 ◽  
Author(s):  
Surendra S. Parmar ◽  
Morley C. Sutter ◽  
Mark Nickerson
Keyword(s):  
Rat Brain ◽  
Cholinesterase Activity ◽  
Succinic Dehydrogenase ◽  
Anterior Lobe ◽  
Subcellular Fractions ◽  
Posterior Pituitary ◽  
Low Concentrations ◽  
Pituitary Glands ◽  
Hydrolysis Of

Fresh rat brains and fresh anterior and posterior pituitary glands of beef were separated by differential centrifugation into subcellular fractions, characterized on the basis of sedimentation and succinic dehydrogenase activity. Cholinesterase activity was measured by both manometric and colorimetric methods, the results of which were comparable. Cholinesterase activity of rat brain was found mainly in the microsome and supernatant fractions. It was quite uniformly distributed in all subcellular fractions of both anterior and posterior pituitary. Comparisons of the relative rates of hydrolysis of acetylthiocholine and butyrylthiocholine, and of inhibition by eserine, indicated that brain contains a much higher percentage of acetylcholinesterase than do both lobes of the pituitary, which contain relatively low concentrations of the specific enzyme. Total cholinesterase activity and its sensitivity to inhibition by eserine in the posterior pituitary were found to be midway between those of the anterior lobe and of the brain, from which the posterior pituitary was derived during embryological development.

Evaluation of rat brain subcellular fractions for the prediction of in vivo drug metabolism in brain

2018 ◽  
Vol 33 (1) ◽  
pp. S79 ◽  
Author(s):  
Mohammad Shadid ◽  
Ying Liu ◽  
Elvana Veizaj ◽  
Jiansheng Huang ◽  
Josh Johnson ◽  
...  
Keyword(s):  
Drug Metabolism ◽  
Rat Brain ◽  
Subcellular Fractions ◽  
In Vivo Drug Metabolism
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