Eleven new microsatellite markers for the tropical sea urchin Tripneustes gratilla and cross-amplification in Tripneustes ventricosa

<p>The present study investigated the influence of different temperature levels (16, 19, 22, 25, 28, 31 and 34oC) on embryonic and early larval development of the tropical sea urchin, <em>Tripneustes gratilla</em> (Linnaeus, 1758) in a controlled laboratory condition. The critical lower and higher temperature for embryonic development was found to be 16 and 34oC, respectively. Embryos reared in these temperatures exhibited 100% abnormality within 48 h post-insemination. The time required to reach embryonic and larval stages was increased with temperature from 28oC followed by 31, 25, 22 and 19oC. The developmental time of 2-cell to 4-arm pluteus larvae showed significant (p<0.05) differences. The survival (%) of larvae at the prism, 2-arm and 4-arm stages were observed as dissimilar from 22 to 34oC, and the highest values (100% or near 100%) were found at 25 and 28oC. The morphometric measurements from prism to 4-arm pluteus larvae at different temperatures differed significantly (p<0.05). However among the temperatures evaluated, 28oC was found as the best temperature for better growth and development of larvae at all stages. The findings of the study would help to develop captive breeding and seed production programmes for commercial aquaculture of the species.</p>

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Oxidative Damage in Response to Natural Levels of UV-B Radiation in Larvae of the Tropical Sea Urchin Tripneustes gratilla

Photochemistry and Photobiology ◽

10.1111/j.1751-1097.2010.00779.x ◽

2010 ◽

Vol 86 (5) ◽

pp. 1091-1098 ◽

Cited By ~ 29

Author(s):

Kathryn Naomi Lister ◽

Miles D. Lamare ◽

David J. Burritt

Keyword(s):

Oxidative Damage ◽

Sea Urchin ◽

Tripneustes Gratilla ◽

Tropical Sea

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Gene expression across tissues, sex, and life stages in the sea urchin Tripneustes gratilla [Toxopneustidae, Odontophora, Camarodonta]

10.1101/284224 ◽

2018 ◽

Author(s):

Áki Jarl Láruson ◽

Simon E. Coppard ◽

Melissa H. Pespeni ◽

Floyd A. Reed

Keyword(s):

Gene Expression ◽

Sea Urchin ◽

Population Biology ◽

De Novo ◽

Expression Profiles ◽

Gene Expression Profiles ◽

High Mobility ◽

Tissue Samples ◽

Tripneustes Gratilla ◽

Tropical Sea

AbstractThe pan-tropical sea urchin Tripneustes gratilla is an ecologically and economically important shallow water algal grazer. The aquaculture of T. gratilla has spurred growing interest in the population biology of the species, and by extension the generation of more molecular resources. To this purpose, de novo transcriptomes of T. gratilla were generated for two adults, a male and a female, as well as for a cohort of approximately 1,000 plutei larvae. Gene expression profiles of three adult tissue samples were quantified and compared. These samples were of gonadal tissue, the neural ring, and pooled tube feet and pedicellariae. Levels of shared and different gene expression between sexes, as well as across functional categories of interest, including the immune system, toxins, genes involved in fertilization, and sensory genes are highlighted. Differences in expression of Sex determining Region Y-related High Mobility Group box groups and general isoform expression between the sexes is observed. Additionally an expansion of the tumor suppressor DMBT1 was observed in T. gratilla when compared to the annotated genome of the sea urchin Strongylocentrotus purpuratus. The draft transcriptome of T. gratilla is presented here in order to facilitate more genomic level analysis of de-novo sea urchin systems.

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