COMPARISON OF THROMBOLYTIC, FIBRINOLYTIC, AND FIBRINOGENOLYTIC PROPERTIES OF TISSUE PLASMINOGEN ACTIVATOR, STREPTOKINASE, SINGLE-CHAIN UROKINASE, HIGH MOLECULAR WEIGHT AND LOW MOLECULAR WEIGHT UROKINASE IN HUMAN PLASMAIN VITRO

SummaryAlthough heparin is often given as an adjunct to tissue plasminogen activator (t-PA), the effect of heparin on t-PA induced fibrin(ogen)olysis is controversial. To address this controversy, we examined the effects of standard and low molecular weight heparin (enoxaparine) on both t-PA induced clot lysis and t-PA mediated fibrinogenolysis in a human plasma system. Accordingly, 125I-labeled fibrin clots were incubated in t-PA containing citrated plasma in the presence or absence of these glycosaminoglycans, and the extent of thrombolysis was determined by measuring residual radioactivity of the clots, while Bβ1–42 levels were used as a specific index of fibrinogenolysis. Over a wide range of t-PA concentrations (0.1 to 1.6 μg/ml), neither heparin nor enoxaparine influences either t-PA induced clot lysis or t-PA mediated Bβ1–42 generation. These findings suggest that either agent could be used as an adjunct to t-PA without compromising either the thrombolytic potential of t-PA or its clotselectivity

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Effect of Low-Molecular-Weight Heparin on Recombinant Tissue Plasminogen Activator-induced Thrombolysis in Canine Pulmonary Embolism

CHEST Journal ◽

10.1378/chest.100.2.464 ◽

1991 ◽

Vol 100 (2) ◽

pp. 464-469 ◽

Cited By ~ 4

Author(s):

Joel Werter ◽

John Ducas ◽

Shian Gu ◽

S. Ming Chan ◽

Richard M. Prewitt

Keyword(s):

Molecular Weight ◽

Pulmonary Embolism ◽

Plasminogen Activator ◽

Tissue Plasminogen Activator ◽

Low Molecular Weight Heparin ◽

Recombinant Tissue Plasminogen Activator ◽

Low Molecular Weight ◽

Tissue Plasminogen

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Differentiation of Proactivator from Tissue Plasminogen Activator with Low Molecular Weight in the Paranasal Mucous Membrane

Auris Nasus Larynx ◽

10.1016/s0385-8146(82)80006-0 ◽

1982 ◽

Vol 9 (2) ◽

pp. 99-104

Author(s):

Tadayoshi Kosugi ◽

Ippei Takagi ◽

Yasuhiro Ariga ◽

Kiyokatsu Kinjo ◽

Shigemitsu Matayoshi ◽

...

Keyword(s):

Molecular Weight ◽

Plasminogen Activator ◽

Tissue Plasminogen Activator ◽

Mucous Membrane ◽

Low Molecular Weight ◽

Tissue Plasminogen

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A Collaborative Study to Establish the 2nd International Standard for Tissue Plasminogen Activator (t-PA)

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646067 ◽

1987 ◽

Vol 58 (04) ◽

pp. 1085-1087 ◽

Cited By ~ 23

Author(s):

P J Gaffney ◽

A D Curtis

Keyword(s):

Plasminogen Activator ◽

Tissue Plasminogen Activator ◽

Collaborative Study ◽

Chinese Hamster ◽

World Health ◽

Clot Lysis ◽

Expert Committee ◽

Single Chain ◽

International Standard ◽

Tissue Plasminogen

SummaryAn international collaborative study involving ten laboratories located in eight different countries was undertaken in order to replace the current International Standard (I.S.) for tissue plasminogen activator (t-PA). Two lyophilised candidate preparations of high purity were assessed in comparison with the current I.S. for t-PA using only a clot lysis assay. One preparation (coded 861670) was purified from a cultured melanoma cell supernatant and was about 98% single chain t-PA while the other preparation (coded 861624) was derived from Chinese hamster ovary (CHO) cells following DNA recombinant procedures and was 75% single chain t-PA.Both candidate preparations of t-PA compared in quite a satisfactory manner with the current I.S. from the viewpoint of the biometrics of parallel line bioassays and both preparations were quite stable for long periods at low temperatures and stable from up to 1 month at temperatures of 20° and 38° C. Both fultil the criteria to serve as a satisfactory Znd International Standard for t-PA. The Fibrinolysis Subcommittee of the International Committee for Thrombosis and Haemostasis recommended the melanoma source t-PA (861670) as the next I.S. in order to maintain continuity with the 1st I.S. which was also a melanomatype preparation. The data from the ten laboratories indicated that each ampoule of the new proposed standard contains 850 international units of t-PA activity by the clot lysis assay. It is planned to present the results of this study to the Expert Committee on Biological Standardization of the World Health Organization at its next meeting and to request that the preparation of t-PA, coded 861670, be established as the 2ndlnternational Standard for t-PA.

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