Histologic Comparison of Canine Skin Biopsies Collected Using Monopolar Electrosurgery, CO2Laser, Radiowave Radiosurgery, Skin Biopsy Punch, and Scalpel

2007 ◽  
Vol 36 (1) ◽  
pp. 50-56 ◽  
Author(s):  
EDWARD B. SILVERMAN ◽  
ROBERT W. READ ◽  
CAROLYN R. BOYLE ◽  
ROBERT COOPER ◽  
WILLIAM W. MILLER ◽  
...  
Keyword(s):  
Skin Biopsy ◽  
Biopsy Punch ◽  
Monopolar Electrosurgery ◽  
Skin Biopsies

scholarly journals Skin biopsy in Paediatric Rheumatology: 10 years review of practice.

2021 ◽  
Author(s):  
Femi Adeniyi ◽  
Kunle Oyedokun
Keyword(s):  
Skin Biopsy ◽  
Medical Records ◽  
Punch Biopsy ◽  
Paediatric Rheumatology ◽  
Excision Biopsy ◽  
Sufficient Information ◽  
Rheumatology Unit ◽  
Skin Biopsies ◽  
Alder Hey ◽  
Age Range

BACKGROUNDSkin biopsy has limited but sometimes distinctly useful place in diagnosis of childhood rheumatologic disorders. For example, skin biopsy is of little value in differentiating lupus from dermatomyositis. The purpose of skin biopsy is to support diagnosis, disease monitoring (e.g.scleroderma) or treatment (e.g., skin cancer). OBJECTIVETo evaluate skin biopsy practices in a tertiary paediatric rheumatology unit in order to determine the usefulness. METHODA retrospective review of medical records of paediatric rheumatology patients who have had skin biopsy over a 10-year period between 2008 and 2018 at Alder Hey Children Hospital.RESULTS & DISCUSSIONA total of 49 skin biopsies with an average of 5 per year was performed. Patients age range from 0.75 -18 years with mean of 10 years. Sites of biopsy and main diagnoses are as shown in figure 1 & 2. Fifty nine percent had excision biopsy while 41% had punch biopsy. Majority of skin biopsies (94%) were performed to aid diagnosis. Severe HSP was the most common indi?cation for skin biopsies requested by rheumatologist. A high proportion of the biopsies (84%) were helpful in the patient care; 55% confirmed clinical suspicions, 25% excluded. CONCLUSION & PERSPECTIVESRheumatologist are selective in the use of skin biopsy for diagnosis. When indicated, skin biopsy is often helpful in the diagnosis and monitoring of rheumatologic disorders. It must be ensured that sufficient information with clear clinico-pathologic question is provided when requesting skin biopsy to enhance positive result.

scholarly journals Minimally invasive sampling to identify leprosy patients with a high bacterial burden in the Union of the Comoros

2021 ◽  
Vol 15 (11) ◽  
pp. e0009924
Author(s):  
Sofie Marijke Braet ◽  
Anouk van Hooij ◽  
Epco Hasker ◽  
Erik Fransen ◽  
Abdou Wirdane ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Skin Biopsy ◽  
Bacterial Load ◽  
World Health ◽  
Blood Samples ◽  
Nasal Swabs ◽  
Leprosy Patients ◽  
Skin Biopsies ◽  
High Bacterial Load ◽  
Union Of The Comoros

The World Health Organization (WHO) endorsed diagnosis of leprosy (also known as Hansen’s disease) entirely based on clinical cardinal signs, without microbiological confirmation, which may lead to late or misdiagnosis. The use of slit skin smears is variable, but lacks sensitivity. In 2017–2018 during the ComLep study, on the island of Anjouan (Union of the Comoros; High priority country according to WHO, 310 patients were diagnosed with leprosy (paucibacillary = 159; multibacillary = 151), of whom 263 were sampled for a skin biopsy and fingerstick blood, and 260 for a minimally-invasive nasal swab. In 74.5% of all skin biopsies and in 15.4% of all nasal swabs, M. leprae DNA was detected. In 63.1% of fingerstick blood samples, M. leprae specific antibodies were detected with the quantitative αPGL-I test. Results show a strong correlation of αPGL-I IgM levels in fingerstick blood and RLEP-qPCR positivity of nasal swabs, with the M. leprae bacterial load measured by RLEP-qPCR of skin biopsies. Patients with a high bacterial load (≥50,000 bacilli in a skin biopsy) can be identified with combination of counting lesions and the αPGL-I test. To our knowledge, this is the first study that compared αPGL-I IgM levels in fingerstick blood with the bacterial load determined by RLEP-qPCR in skin biopsies of leprosy patients. The demonstrated potential of minimally invasive sampling such as fingerstick blood samples to identify high bacterial load persons likely to be accountable for the ongoing transmission, merits further evaluation in follow-up studies.

Skin biopsies predict acute graft-versus-host disease after small bowel transplantation in pigs

2007 ◽  
Vol 55 (4) ◽  
pp. 533-541 ◽  
Author(s):  
Tadeja Pintar ◽  
A. Pleskovič ◽  
M. Alessiani ◽  
Francesca Abbiati ◽  
Aleksandra Milutinović ◽  
...  
Keyword(s):  
Small Bowel ◽  
Skin Biopsy ◽  
Prognostic Value ◽  
Acute Gvhd ◽  
Small Bowel Transplantation ◽  
Histological Grading ◽  
Histological Changes ◽  
Graft Versus Host ◽  
Skin Biopsies ◽  
Diagnostic And Prognostic Value

Intestinal transplantation is being increasingly performed to treat patients with irreversible intestinal failure. The major cause of intestinal graft failure is graft-versus-host disease (GVHD) that represents a life-threatening complication after small bowel transplantation (Itx). The purpose of this study was to assess the diagnostic and prognostic value of skin biopsy histological changes for acute GVHD after Itx in pigs. Thirty-four Large White pigs were divided into three groups: Group 1 with Itx only, Group 2 with Itx and donor bone marrow infusion (Itx BM) and Group 3 (control group — before the operation). Animals received tacrolimus-based immunosuppression from day 0 to day 30 postoperatively. Skin and small bowel biopsies were histologically assessed, analysed and classified from grade 1 to 4 on postoperative days 15, 30, 45 and 60. There was a strong correlation between the histological grading values of skin biopsy changes and the histological grading values of small bowel biopsy changes (Kendall’s tau_b is 0.855 for the Itx group and 0.730 for the Itx BM group). The significant correlation found between skin and small bowel histological changes suggests the prognostic value of skin biopsies after Itx. In conclusion, our findings emphasise the diagnostic and prognostic value of skin biopsy analysis for acute GVHD after Itx.

Limited Role of Random Skin Biopsy in the Diagnosis of Intravascular Lymphoma in Adult Patients with Hemophagocytic Lymphohistiocytosis

2017 ◽  
Vol 138 (1) ◽  
pp. 33-38 ◽  
Author(s):  
Hyunje G. Cho ◽  
Sarah L. Sheu ◽  
Karen Y. Kuo ◽  
Mina S. Ally ◽  
Elizabeth E. Bailey ◽  
...  
Keyword(s):  
Skin Biopsy ◽  
Hemophagocytic Lymphohistiocytosis ◽  
Normal Skin ◽  
Retrospective Chart Review ◽  
Adult Patients ◽  
Intravascular Lymphoma ◽  
Clinically Normal ◽  
Limited Role ◽  
Skin Biopsies

Background/Aims: This study examined the role of random normal skin biopsy in the diagnosis of intravascular lymphoma (IVL) in adult Western patients with clinically diagnosed hemophagocytic lymphohistiocytosis (HLH). Methods: In a retrospective chart review study, we analyzed a total of 59 skin biopsies that were performed to diagnose IVL in 21 adult patients with HLH seen at Stanford Hospital between 2004 and 2016. Results: Out of the 59 skin biopsies, 42 were taken from clinically normal-appearing skin and 17 from clinically abnormal-appearing skin. None of the 59 biopsies revealed a diagnosis of primary or metastatic malignancy, regardless of the malignancy history, clinical presentation, and biopsy and histopathologic characteristics. A review of 8 positive IVL cases at Stanford Hospital including 1 case associated with HLH showed 1 positive diagnosis by a targeted skin biopsy and other positive diagnoses by bone marrow (n = 4), lung (n = 2), brain (n = 2), muscle (n = 1), and nerve (n = 1). Conclusion: Random skin biopsies have a limited role in diagnosing IVL in adult patients with HLH, in the setting of a single academic institution in the USA. A review of the literature emphasizes the role of a full body skin exam with a selective skin biopsy in these patients.

scholarly journals Is idiopathic hirsutism (IH) really idiopathic? mRNA expressions of skin steroidogenic enzymes in women with IH

2015 ◽  
Vol 173 (4) ◽  
pp. 447-454 ◽  
Author(s):  
Serpil Taheri ◽  
Gokmen Zararsiz ◽  
Sulbiye Karaburgu ◽  
Murat Borlu ◽  
Mahmut Tuncay Ozgun ◽  
...  
Keyword(s):  
Skin Biopsy ◽  
Mrna Expression ◽  
Androgen Excess ◽  
Functional Roles ◽  
Steroid Sulphatase ◽  
Healthy Women ◽  
Androgen Synthesis ◽  
Idiopathic Hirsutism ◽  
Skin Biopsies ◽  
Gene Mrna

ObjectiveHirsutism results from hyperandrogenemia and/or exaggerated androgen responsiveness. Among various causes of hirsutism, some patients do not exhibit androgen excess which is called idiopathic hirsutism (IH). The pathogenesis of IH could not so far be clearly established.DesignTo investigate the mRNA expression of aromatase enzyme and the other enzymes having functional roles in the steroidogenic pathway, in freshly obtained skin tissue from subumbilical skin and the arm of the patients with IH and healthy women.MethodsTwenty-one women with IH and 15 healthy women were included in the study. We aimed to determine mRNA expressions of genes associated with local androgen synthesis and metabolism (CYP11A1, STS, CYP19A1, SRD5A1, SRD5A2, HSD3B1, AR, COMT, ESR1, ESR2, HSD3B2, CYP17A1, SULT2A1, SULT1E1, HSD17B2, IL6, TGFB1, TNFA) from skin biopsy and blood samples of patients with IH and the data compared with healthy subjects.ResultsPatients with IH exhibit significantly lower interleukin 6 (IL6) mRNA expression and higher steroid sulphatase (STS) and hydroxysteroid (17beta) dehydrogenase 2 (HSD17B2), gene mRNA expression, respectively, in the subumbilical region skin biopsies. Similarly, patients with IH exhibit significantly lowerIL6mRNA expression and higherSTSandHSD17B2gene mRNA expression, respectively, in the arm skin compared to healthy women's subumbilical region.ConclusionsIn both arm and subumbilical skin biopsy of patients with IH, we observed an up-regulation ofHSD17B2andSTS, decreasedIL6mRNA expression, probably determining an increase in the local amount of active androgens, which could then be used as substrate for other androgen metabolic routes.

A New Application for the Disposable Skin Biopsy Punch

1981 ◽  
Vol 92 (5) ◽  
pp. 737
Author(s):  
Joseph W. Sassani ◽  
Jonathan Cutler
Keyword(s):  
Skin Biopsy ◽  
Biopsy Punch

scholarly journals Usefulness of disposable skin biopsy punch for cervical occlusion after cervical conization

2020 ◽  
Vol 9 (2) ◽  
pp. 95
Author(s):  
Rina Funada ◽  
Kazushige Adachi ◽  
Yoshimitsu Yamamoto ◽  
Itsuko Nakamichi
Keyword(s):  
Skin Biopsy ◽  
Biopsy Punch ◽  
Cervical Conization

Prospective Step Sections for Small Skin Biopsies

2007 ◽  
Vol 131 (1) ◽  
pp. 107-111
Author(s):  
Andrea K. Bruecks ◽  
Jill M. Shupe ◽  
Martin J. Trotter
Keyword(s):  
Diagnostic Accuracy ◽  
Skin Biopsy ◽  
Turnaround Time ◽  
Biopsy Specimens ◽  
Paraffin Block ◽  
Skin Biopsies ◽  
Level 1

Abstract Context.—In our laboratory, for small skin biopsies or curetted specimens, 3 slides are prepared before the case is reviewed by the dermatopathologist. Objective.—To examine the utility of these “prospective” step sections in improving diagnostic accuracy and turnaround time. Design.—Five hundred consecutive cases, in which step sections had been cut prior to slide review, were studied. For each specimen, 3 slides, each consisting of 1 ribbon of tissue containing 4 to 6 sections, were obtained at 50-μm intervals from the paraffin block. Results.—Fifty-eight biopsies (12%) were nondiagnostic using slide 1 alone. Step sections provided a diagnosis in 19 of 58 cases. In an additional 15 cases (3%) in which a diagnosis was possible using slide 1, deeper levels resulted in a change in diagnosis. Thus, in 34 (7%) of 500 biopsies, deeper levels resulted in improved diagnostic accuracy. In addition, the pathologist would have ordered step sections in a further 117 cases (23%) to clarify the diagnosis rendered on level 1 or to exclude other lesions. Thus, 30% of small skin biopsies would have required deeper levels if step sections had not been obtained prior to slide review. Conclusions.—In our laboratory, the use of prospective step sections is essentially cost-neutral and case turnaround time is improved by 9% to 45%. Step sections result in a changed diagnosis in 7% of small skin biopsy specimens.

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