Characterization of Sarcocystis falcatula Isolates from the Argentinian Opossum, Didelphis albiventris

2000 ◽  
Vol 47 (3) ◽  
pp. 260-263 ◽  
Author(s):  
J. P. DUBEY ◽  
D. S. LINDSAY ◽  
L. VENTURINI ◽  
C. VENTURINI
Keyword(s):  
Sarcocystis Falcatula ◽  
Didelphis Albiventris

scholarly journals Isolates of Sarcocystis falcatula-like Organisms from South American Opossums Didelphis marsupialis and Didelphis albiventris from Sao Paulo, Brazil

2001 ◽  
Vol 87 (6) ◽  
pp. 1449 ◽  
Author(s):  
J. P. Dubey ◽  
D. S. Lindsay ◽  
B. M. Rosenthal ◽  
C. E. Kerber ◽  
N. Kasai ◽  
...  
Keyword(s):  
Sao Paulo ◽  
South American ◽  
São Paulo ◽  
Sarcocystis Falcatula ◽  
Didelphis Albiventris

Multilocus characterization of Sarcocystis falcatula -related organisms isolated in Brazil supports genetic admixture of high diverse SAG alleles among the isolates

2018 ◽  
Vol 188 ◽  
pp. 42-49 ◽  
Author(s):  
Marina O. Cesar ◽  
Eliana R. Matushima ◽  
Ticiana Zwarg ◽  
Alice S. de Oliveira ◽  
Thaís C. Sanches ◽  
...  
Keyword(s):  
Genetic Admixture ◽  
Sarcocystis Falcatula

scholarly journals Biochemical, Pharmacological, and Structural Characterization of New Basic Bbil-TX fromBothriopsis bilineataSnake Venom

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Victor Corasolla Carregari ◽  
Rafael Stuani Floriano ◽  
Lea Rodrigues-Simioni ◽  
Flavia V. Winck ◽  
Paulo Aparecido Baldasso ◽  
...  
Keyword(s):  
Snake Venom ◽  
Protein Sequence ◽  
Maximal Activity ◽  
Optimal Conditions ◽  
High Identity ◽  
Phospholipid Hydrolysis ◽  
Rp Hplc ◽  
Crotalus Durissus ◽  
Didelphis Albiventris

Bbil-TX, a PLA2, was purified fromBothriopsis bilineatasnake venom after only one chromatographic step using RP-HPLC onμ-Bondapak C-18 column. A molecular mass of 14243.8 Da was confirmed by Q-Tof Ultima API ESI/MS (TOF MS mode) mass spectrometry. The partial protein sequence obtained was then submitted to BLASTp, with the search restricted to PLA2from snakes and shows high identity values when compared to other PLA2s. PLA2activity was presented in the presence of a synthetic substrate and showed a minimum sigmoidal behavior, reaching its maximal activity at pH 8.0 and 25–37∘C. Maximum PLA2activity required Ca2+and in the presence of Cd2+, Zn2+, Mn2+, and Mg2+it was reduced in the presence or absence of Ca2+. Crotapotin fromCrotalus durissus cascavellarattlesnake venom and antihemorrhagic factor DA2-II fromDidelphis albiventrisopossum sera under optimal conditions significantly inhibit the enzymatic activity. Bbil-TX induces myonecrosis in mice. The fraction does not show a significant cytotoxic activity in myotubes and myoblasts (C2C12). The inflammatory events induced in the serum of mice by Bbil-TX isolated fromBothriopsis bilineatasnake venom were investigated. An increase in vascular permeability and in the levels of TNF-a, IL-6, and IL-1 was was induced. Since Bbil-TX exerts a stronger proinflammatory effect, the phospholipid hydrolysis may be relevant for these phenomena.

scholarly journals MORPHOLOGICAL AND GENETIC CHARACTERIZATION OF NEMATODES OF THE OPOSSUM Didelphis albiventris AND THE ARMADILLO Dasypus novemcinctus FROM SERRA DA CAPIVARA NATIONAL PARK

2019 ◽  
Vol 23 (02) ◽  
pp. 315-332
Author(s):  
Everton Gustavo Nunes dos Santos ◽  
Vanessa Aparecida das Chagas Moutinho ◽  
Marcia Chame ◽  
Claudia Portes Santos
Keyword(s):  
National Park ◽  
Genetic Characterization ◽  
Dasypus Novemcinctus ◽  
Didelphis Albiventris

Isolation of Sarcocystis falcatula from the South American opossum (Didelphis albiventris) from Argentina

1999 ◽  
Vol 86 (4) ◽  
pp. 239-244 ◽  
Author(s):  
J.P. Dubey ◽  
L. Venturini ◽  
C. Venturini ◽  
W. Basso ◽  
J. Unzaga
Keyword(s):  
South American ◽  
The South ◽  
American Opossum ◽  
Sarcocystis Falcatula ◽  
Didelphis Albiventris

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

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