Isolation and characterization of dental pulp stem cells from a supernumerary tooth

2008 ◽  
Vol 37 (9) ◽  
pp. 571-574 ◽  
Author(s):  
Anderson Hsien-Cheng Huang ◽  
Yuk-Kwan Chen ◽  
Lin-Min Lin ◽  
Tien-Yue Shieh ◽  
Anthony Wing-Sang Chan
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Supernumerary Tooth ◽  
Isolation And Characterization

Isolation and Characterization of Dental Pulp Stem Cells from a Patient with Papillon–Lefèvre Syndrome

2013 ◽  
Vol 39 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Pakize Neslihan Taşlı ◽  
Sıdıka Tapşın ◽  
Sezin Demirel ◽  
Mehmet Emir Yalvaç ◽  
Serap Akyuz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Isolation And Characterization

scholarly journals Characterization of Odontoblasts in Supernumerary Tooth-derived Dental Pulp Stem Cells between Passages by Real-Time PCR

2021 ◽  
Vol 48 (3) ◽  
pp. 291-301
Author(s):  
Sangeun Ji ◽  
Sol Song ◽  
Joonhaeng Lee ◽  
Jongbin Kim ◽  
Jongsoo Kim
Keyword(s):  
Stem Cells ◽  
Real Time ◽  
Dental Pulp ◽  
Matrix Protein ◽  
Additive Group ◽  
Dental Pulp Stem Cells ◽  
Supernumerary Teeth ◽  
Supernumerary Tooth ◽  
Passage Cells

The aim of this study is to compare the properties of odontoblast gene of early passage cells and late passage cells derived from impacted maxillary supernumerary teeth. Impacted supernumerary teeth with maxilla were extracted from 12 patients (8 males, 4 females) between 6 - 9 years old without medical history. Real-time polymerase chain reaction (PCR) was conducted to compare characterization of odontoblast cell in the 3rd and 10th passage, and between with bone inducing additive group and without additive group. Genes for odontoblasts characteristics are osteonectin (ONT), alkaline phosphatase (ALP), osteocalcin (OCN), dentin matrix protein 1 (DMP-1) and dentin sialophosphoprotein (DSPP). The level of gene expression was in a decreasing order of ONT, ALP, OCN, DMP-1 and DSPP in the 3rd passage, and in decreasing order of ONT, DMP-1, OCN, ALP, and DSPP in the 10th passage in the undifferentiation and differentiation group. The order of ONT, DMP-1, and OCN did not changed. ALP and DMP-1 were switched in order. ALP and DMP-1 may be used as important markers for differentiating between the 3rd passage and 10th passage cells. Considering that supernumerary tooth was extracted young age and the time required to cultured 10th passage was short, supernumerary tooth can be considered a useful donor site of dental pulp stem cells.

Isolation and Characterization of Dental Pulp Stem Cells from Murine Incisors

2014 ◽  
Vol 14 (4) ◽  
pp. 327-331 ◽  
Author(s):  
M.R. Nur Akmal ◽  
Z.A. Intan Zari ◽  
M.A.W. Rohaya ◽  
S. Sahidan ◽  
Z.A. Zaidah ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of equine dental pulp stem cells derived from Thoroughbred wolf teeth

2017 ◽  
Vol 79 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Shingo ISHIKAWA ◽  
Chie HORINOUCHI ◽  
Daiki MURATA ◽  
Shota MATSUZAKI ◽  
Kazuhiro MISUMI ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Isolation And Characterization

Isolation and characterization of human dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHED) – an in vitro study

2018 ◽  
Vol 10 (2) ◽  
pp. 9-18
Author(s):  
Dr. Vinay Rao ◽  
Dr. Roopa R Nadig ◽  
Dr.Ramanand Nadig ◽  
Dr.Karthik .J ◽  
Dr. Veena Pai S
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Surface Characterization ◽  
Dental Pulp Stem Cells ◽  
In Vivo Studies ◽  
Deciduous Teeth ◽  
Pulp Tissue ◽  
Isolation And Characterization

AIMS AND OBJECTIVES: 1. Isolation and growth of dental pulp stem cells (DPSCs) and stem cells from exfoliated human deciduous teeth (SHED). 2. Characterization of dental pulp stem cells (DPSCs) and stem cells from exfoliated human deciduous teeth (SHED). METHODS: The pulp tissue was digested in collagenase and cultured in DMEM Dulbecco’s Modified Eagle’s Media). The stem cells were identified and isolated. Surface characterization of cells was done with the help of flow cytometer using a panel of various surface markers. An immuno cytochemistry analysis was done to see the expression of proteins in the cells. RESULTS: Identification of cells was done with the help of a phase contrast microscope. Flow cytometry analyses for various CD markers showed similar results for both DPSCs and SHED. The cells showed positive expression for pluripotent markers, ectodermal markers and mesodermal markers. CONCLUSION: The study demonstrated that stem cells existed in human deciduous and permanent pulp tissue. The stem cells present in deciduous permanent pulp tissue can be isolated, cultivated and expanded in vitro. Both DPSCs and SHED show almost a similar expression pattern profile for variety of antigens tested. Further studies should include analysis of diverse cell populations to elucidate their potential to differentiate into various cell types followed by in vivo studies in animals and humans.

Preparation and characterization of titanium—segmented polyurethane composites for bone tissue engineering

2018 ◽  
Vol 33 (1) ◽  
pp. 11-22 ◽  
Author(s):  
Fernando Javier Aguilar-Perez ◽  
Rossana Vargas-Coronado ◽  
Jose Manuel Cervantes-Uc ◽  
Juan Valerio Cauich-Rodriguez ◽  
Raul Rosales-Ibañez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Soft Segment ◽  
Physicochemical Characterization ◽  
Dental Pulp Stem Cells ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
Human Dental Pulp ◽  
Polyurethane Composites

Segmented polyurethanes were prepared with polycaprolactone diol as soft segment and 4,4-methylene-bis cyclohexyl diisocyanate and l-glutamine as the rigid segment. These polyurethanes were filled with 1 wt.% to 5 wt.% titanium particles (Ti), physicochemically characterized and their biocompatibility assessed using human dental pulp stem cells and mice osteoblasts. Physicochemical characterization showed that composites retained the properties of the semicrystalline polyurethane as they exhibited a glass transition temperature (Tg) between −35°C and −45°C, melting temperature (Tm) at 52°C and crystallinity close to 40% as determined by differential scanning calorimetry. In agreement with this, X-ray diffraction showed reflections at 21.3° and 23.6° for polycaprolactone diol and reflections at 35.1°, 38.4°, and 40.2° for Ti particles suggesting that these particles are not acting as nucleating sites. The addition of up to 5 wt.% of Ti reduced both, tensile strength and maximum strain from 1.9 MPa to 1.2 MPa, and from 670% to 172% for pristine and filled polyurethane, respectively. Although there were differences between composites at low strain rates, no significant differences in mechanical behavior were observed at higher strain rate where a tensile stress of 8.5 MPa and strain of 223% were observed for 5 wt.% composites. The addition to titanium particles had a beneficial effect on both human dental pulp stem cells and osteoblasts viability, as it increased with the amount of titanium in composites up to 10 days of incubation.

Sign in / Sign up

Export Citation Format

Share Document