ES08.02 Bacterial contamination of cellular blood components: risks, sources and control

Background: Besides enhancing healing, an ideal dressing should prevent invasion of pathogens and control the number of bacteria already present in the wounds. Objective: To evaluate the barrier and antimicrobial properties of a cyanoacrylate-based bandage (LAB) against Staphylococcus aureus or Pseudomonas aeruginosa on partial thickness wounds in swines. Methods: Barrier study: Bacteria were inoculated over test materials (LAB, standard bandage, air-exposed) that were placed over wounds. The bacteria from wounds were quantitated at 24, 48, and 72 hours postinoculation. Antimicrobial study: Wounds inoculated with bacteria were covered with LAB, standard bandage, or hydrocolloid bandage or left air-exposed. The bacteria recovered from wounds were quantitated at 24 and 72 hours after treatment. Results: Barrier study: No bacteria were recovered from LAB-treated wounds. Antimicrobial study: LAB reduced the number of inoculated bacteria in comparison to all other groups. Conclusion: LAB is effective in protecting wounds from external bacterial invasion and reducing bacterial contamination.

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Blood and blood components in the prevention and control of bleeding

JAMA ◽

10.1001/jama.202.10.967 ◽

1967 ◽

Vol 202 (10) ◽

pp. 967-969 ◽

Cited By ~ 1

Author(s):

P. J. Schmidt

Keyword(s):

Prevention And Control ◽

Blood Components ◽

And Control

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Endogenous Bacterial Contamination of Plant Tissue Culture Materials: Identification and Control Strategy

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v28i1.37202 ◽

2018 ◽

Vol 28 (1) ◽

pp. 99-108 ◽

Cited By ~ 1

Author(s):

Mohammad Ali ◽

Shefali Boonerjee ◽

Mohammad Nurul Islam ◽

Mihir Lal Saha ◽

M Imdadul Hoque ◽

...

Keyword(s):

Tissue Culture ◽

Plant Tissue Culture ◽

Plant Tissue ◽

Bacterial Contamination ◽

Antibiotic Sensitivity ◽

Sensitivity Test ◽

Bacterial Isolates ◽

Culture And Sensitivity ◽

And Control

The endogenous bacterial contamination of plant tissue culture materials and their possible control was studied. Nine bacterial isolates were isolated from the contaminated tissue culture materials viz. potato and tea. On the basis of morphology and biochemical characters of nine isolates, seven were identified as Gram positive belonging to Bacillus alcalophilus, B. circulans, B. infantis, B. lentus, B. schlegelii, B. pumilus and B. subtilis. Remaining two were Gram negative and identified as Enterobacter cloacae sub. sp. dissolvens and Pantoea agglomerans. Molecular analysis was conducted on the basis of 16S rDNA sequence to confirm three isolates. Culture and sensitivity test was carried out to screen out the antibiotic sensitivity where streptomycin (S-10), polymyxin (PB-300) and gentamicin (CN-120) antibiotics were found to be effective against all bacterial isolates. The culture and sensitivity test reflected the feasibility to control or eliminate the contaminant bacteria during in vitro culture of plant which is very much required in the commercial tissue culture production.Plant Tissue Cult. & Biotech. 28(1): 99-108, 2018 (June)

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