Barrier and Antibacterial Properties of 2-Octyl Cyanoacrylate-Derived Wound Treatment Films

Background: Besides enhancing healing, an ideal dressing should prevent invasion of pathogens and control the number of bacteria already present in the wounds. Objective: To evaluate the barrier and antimicrobial properties of a cyanoacrylate-based bandage (LAB) against Staphylococcus aureus or Pseudomonas aeruginosa on partial thickness wounds in swines. Methods: Barrier study: Bacteria were inoculated over test materials (LAB, standard bandage, air-exposed) that were placed over wounds. The bacteria from wounds were quantitated at 24, 48, and 72 hours postinoculation. Antimicrobial study: Wounds inoculated with bacteria were covered with LAB, standard bandage, or hydrocolloid bandage or left air-exposed. The bacteria recovered from wounds were quantitated at 24 and 72 hours after treatment. Results: Barrier study: No bacteria were recovered from LAB-treated wounds. Antimicrobial study: LAB reduced the number of inoculated bacteria in comparison to all other groups. Conclusion: LAB is effective in protecting wounds from external bacterial invasion and reducing bacterial contamination.

Download Full-text

Synthesis of CoFe 2 O 4 /BiOI Nanocomposite: Effects on Staphylococcus aureus, Escherichia coli, Pseudomonas Aeruginosa and Bacillus Cereus

10.21203/rs.3.rs-842251/v1 ◽

2021 ◽

Author(s):

Davood Gheidari ◽

Morteza Mehrdad ◽

Saloomeh Maleki ◽

Samanesadat Hosseini

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Bacillus Cereus ◽

Antibacterial Properties ◽

Limited Area ◽

X Ray Diffraction ◽

X Ray ◽

Inhibition Concentration ◽

Resolution Mapping

Abstract With the increase of general knowledge and the advancement of science and technology, antibacterial substances were used more than antibiotics. In our current study, the antibacterial virtues of CFO/BiOI nanocomposite were investigated due to its high importance on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus cereus. MIC, MBC , Disk Diffusion and IC50 tests Cefalotin (CF), Amoxicillin (AMX), Gentamicin (GM), Trimethoprim-sulphamethoxazole (SXT) and Ceftriaxone (CRO) antibiotics in concentration 30W, 10i, 10t , 25h and 30 were used to find the antibacterial properties of the synthesized nanocomposite, respectively. For the synthesis of nanocomposites polyethylene glycol (PEG) and sulfonic acid was used as a solvent. It is noteworthy that the synthesis was performed by heat dissolution method without the presence of surfactant. Also, various techniques such as X-Ray Diffraction(XRD), Scanning Electron Microscope (SEM), High resolution mapping and Energy Dispersive X-ray spectroscopy (EDAX) have been used to determine the properties of produced nanocomposites. SEM test results showed that the formed nanoparticles were globular and their size was limited area of 22 to 34 nm. The results showed CFO / BiOI nanocomposite exhibits strong significant biological activity against Bacillus cereus. The results of MBC (Minimum Bactericidal Concentration) and MIC (Minimum Inhibition Concentration) tests for CFO/BiOI nanocomposites on bacteria were examined in the range of 0.12-0.48 mg/ml and 0.06 to 0.24 mg/ml respectively. According to the results, the minimum IC50 value was determined at a concentration of 0.061 mg/ml. On the other hand, the most resisting and susceptible bacteria in this method were Pseudomonas aeruginosa and Bacillus cereus, respectively. These findings are identical to those of a prior study on CoFe2O4 nanoparticles antibacterial properties. MBC of the nanocomposites, 50 µl from all the tubes that showed no obvious bacterial growth were distributed on BHI agar plates and incubated for 24 h at 37 ◦C. The MBC endpoint is defined as the lowest concentration which killed 98% of the bacterial population.

Download Full-text

Antimicrobial Activity of Cultivable Endophytic and Rhizosphere Fungi Associated with “Mile-a-Minute,” Mikania cordata (Asteraceae)

BioMed Research International ◽

10.1155/2020/5292571 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Pavithra L. Jayatilake ◽

Helani Munasinghe

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Antimicrobial Properties ◽

Pharmaceutical Products ◽

Diffusion Method ◽

Molecular Characteristics ◽

Disk Diffusion Method ◽

Rhizosphere Fungi

Endophytic and rhizosphere fungi are understood to be aiding the host plant to overcome a range of biotic and abiotic stresses (nutrition depletion, droughts, etc.) hence, they remain to be reservoirs of plethora of natural products with immense use. Consequently, this investigation of endophytic and rhizosphere fungi isolated from Mikania cordata (a perennial vine that is well established in Sri Lanka) for their antimicrobial properties was performed with the aim of future derivation of potential beneficial pharmaceutical products. Leaves, twigs, and roots of M. cordata were utilized to isolate a total of 9 endophytic fungi out of which the highest amount (44%) accounted was from the twigs. A sample of the immediate layer of soil adhering to the root of M. cordata was utilized to isolate 15 rhizosphere fungi. Fusarium equiseti and Phoma medicaginis were endophytes that were identified based on colony and molecular characteristics. The broad spectrum of antimicrobial activity depicted by F. equiseti (MK517551) was found to be significantly greater (p≤0.05, inhibitory against Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 25853) than P. medicaginis (MK517550) (inhibitory against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 25853) as assessed using the Kirby-Bauer disk diffusion method. Trichoderma virens and Trichoderma asperellum were rhizospere fungi that exhibited remarkable antimicrobial properties against the test pathogens chosen for the study. T. asperellum indicated significantly greater bioactivity against all four bacterial pathogens and Candida albicans ATCC 10231 under study. The ranges of minimum inhibitory concentrations (MICs) of the fungi depicting antimicrobial properties were determined. The results obtained suggest that F. equiseti, P. medicaginis, T. asperellum, and T. virens of M. cordata harness bioprospective values as natural drug candidates. This is the first report on isolation and evaluation of the antimicrobial properties of endophytic and rhizosphere fungi of Mikania cordata.

Download Full-text

The Study of Antibacterial Properties of Anbarnasara Smoke on Multi-Drug Resistant Bacteria Isolated From Urinary Infection in Pregnant Women

International Journal of Enteric Pathogens ◽

10.34172/ijep.2020.24 ◽

2020 ◽

Vol 8 (4) ◽

pp. 110-115

Author(s):

Afsaneh Molamirzaei ◽

Maryam Allahdadian ◽

Monir Doudi

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Pregnant Women ◽

Urinary Infection ◽

Antibacterial Properties ◽

Resistant Bacteria ◽

Klebsiella Pneumonia ◽

Drug Resistant

Background: Using smoke from burning donkey dung has been popular in the treatment of many diseases in Iran. Objective: This study aimed to investigating the antimicrobial properties of donkey dung smoke on multi-drug resistant (MDR) bacteria isolated from urinary infection. Materials and Methods: First, 300 and 200 urine samples were collected from pregnant and non-pregnant women in Isfahan, Iran. Then in each group, 100 bacterial isolates including Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Staphylococcus saprophyticus were isolated. Antibiotic resistant protocol was determined by antibiogram test. Donkey dung was sterilized, disintegrated, and heated. The smokes were concentrated in n-hexane solvent (65%) and were collected after evaporation of the solvent. Finally, the antibacterial activities of the concentrations of 0.25, 0.5 and 1 mg/mL of the smokes were detected using disk diffusion and macrodilution methods. Results: The most abundant MDR isolates causing urinary infections in pregnant and non-pregnant women was Escherichia coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of donkey dung smoke on MDR isolates from pregnant women were 0.25 mg/mL and 0.5 mg/mL, respectively. In the case of MDR isolates in non-pregnant women, the MIC of the smoke on Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus was 0.25 mg/mL, and the MBC on these isolates was 0.5 mg/mL. Conclusion: The smokes from donkey dung investigated in the present study have suitable potentials for controlling the infections after In vivo analysis.

Download Full-text

Antimicrobial Properties of Gallium(III)- and Iron(III)-Loaded Polysaccharides Affecting the Growth of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, In Vitro

ACS Applied Bio Materials ◽

10.1021/acsabm.0c00811 ◽

2020 ◽

Vol 3 (11) ◽

pp. 7589-7597

Author(s):

Mark G. Best ◽

Cassilda Cunha-Reis ◽

Alexey Y. Ganin ◽

Aureliana Sousa ◽

Jenna Johnston ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Properties

Download Full-text

In Vitro Determination of the Antimicrobial Properties of Two Cyanoacrylate Preparations

Journal of Dental Research ◽

10.1177/00220345750540031501 ◽

1975 ◽

Vol 54 (3) ◽

pp. 500-503 ◽

Cited By ~ 9

Author(s):

Gary N. Blum ◽

William A. Nolte ◽

Paul B. Robertson

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Lactobacillus Casei ◽

Antimicrobial Properties ◽

Plate Technique ◽

Inhibition Of Growth ◽

And Staphylococcus Aureus

Isobutyl and trifluoro cyanoacrylates showed varying degrees of inhibition for Lactobacillus casei and Staphylococcus aureus when tested by the spread plate technique. Candida albicans and Pseudomonas aeruginosa were resistant. The results tend to support the view that inhibition of growth was due to the vapor effect and not the diffusibility of the cyanoacrylates.

Download Full-text

Phytochemical and Antimicrobial Properties of Leaves ofAlchonea Cordifolia

E-Journal of Chemistry ◽

10.1155/2010/784095 ◽

2010 ◽

Vol 7 (3) ◽

pp. 1071-1079 ◽

Cited By ~ 5

Author(s):

N. J. George ◽

I. B. Obot ◽

A. N. Ikot ◽

A. E. Akpan ◽

N. O. Obi-Egbedi

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Pathogenic Bacteria ◽

Leaf Extract ◽

Antimicrobial Properties ◽

Ethanolic Extract ◽

Zone Of Inhibition ◽

Bioactive Substances ◽

Butanol Fraction ◽

Zones Of Inhibition

The phytochemistry ofAchornea cordifolialeaf extract using different solvents was studied using standard methods. The effects of the leaf extract on some pathogenic bacteria and fungi were also examined. The Phytochemical screening of the leaves shows the presence of useful ethno-botanical bioactive substances such as tannin, saponin, flavonoid, cardiac glycoside and anthraquinone, while alkaloid, phlobatanin and terpene also tested for were absent. The butanol fraction of the extract gives the highest zone of inhibition (13.0 mm) againstStaphylococcus aureus, Escherichia coliandpseudomonas aeruginosa, which are in parity. The control, gentamycin injection gives 12.5 mm, 11.0 mm and 12.0 mm respective zones of inhibition against theStaphylococcus aureus, Eschariclia coliandPseudomonas aeruginosa. Also, the butanol fraction of the extract shows highest zone of inhibition of 17.0 mm againstCandida albican, while ethanolic extract gives 13.0 mm zone of inhibition againstTrichophyton violaceum.

Download Full-text

Antimicrobial Properties of 6-Bromoeugenol and Eugenol

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.53.57 ◽

2016 ◽

Vol 53 ◽

pp. 57-64

Author(s):

Radia Mahboub ◽

Faiza Memmou

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Inhibitory Concentration ◽

Antimicrobial Properties ◽

Diffusion Method ◽

Disc Diffusion ◽

Bacterial Strains ◽

Disc Diffusion Method ◽

Modest Activity

We have studied the antimicrobial properties of 6-bromoeugenol and eugenol by three strains:Pseudomonas aeruginosa(S1),Escherichia coli(S2) andStaphylococcus aureus(S3). We have determined the minimum inhibitory concentration (MIC) for a range of concentrations using the disc diffusion method. We note that all samples present an antimicrobial activity toward the tested bacterial strains at different concentrations (1, 0.5 and 0.25 mg/ml). The 6-bromoeugenol gives modest activity with (S1) and (S3). Eugenol reacts positively with thePseudomonas aeruginosa(S1) at all concentrations and with theEscherichiacoli(S2) at 0.5 mg/ml. We remark that thePseudomonas aeruginosa(S1) is the more sensitive strain thanEscherichiacoli(S2) andStaphylococcus aureus(S3). We have estimated the activity coefficient which has confirmed the antimicrobial activity of the different samples. So, 6-bromoeugenol has shown his efficiency as antimicrobial agent.

Download Full-text

Antimicrobial properties of ZnO nanoparticles incorporated in polyurethane varnish

Processing and Application of Ceramics ◽

10.2298/pac1101041z ◽

2011 ◽

Vol 5 (1) ◽

pp. 41-45 ◽

Cited By ~ 25

Author(s):

Dusan Zvekic ◽

Vladimir Srdic ◽

Maja Karaman ◽

Milan Matavulj

Keyword(s):

Escherichia Coli ◽

Saccharomyces Cerevisiae ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Zno Nanoparticles ◽

Antimicrobial Properties ◽

Antimicrobial Effect ◽

Plate Test ◽

Sacharomyces Cerevisiae

The antimicrobial effect of the ZnO nanoparticles dispersed in polyurethane varnishes was investigated. Antimicrobial activity was evaluated against three bacteria (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) and one strain fungi (Sacharomyces cerevisiae) by the 'pour-plate' test. It was shown that the ZnO nanoparticles efficiently inhibit growth of the colonies of Staphylococcus aureus, Pseudomonas aeruginosa and Saccharomyces cerevisiae, but their activity against Escherichia coli was found to be questionable suggesting more detailed research.

Download Full-text

Antibacterial properties of a biologically active composite based on glauconite

Izvestiya of Saratov University New Series Series Chemistry Biology Ecology ◽

10.18500/1816-9775-2021-21-1-62-71 ◽

2021 ◽

Vol 21 (1) ◽

pp. 62-71

Author(s):

Sergei B. Venig ◽

◽

Rimma K. Chernova ◽

Victor G. Sergeantov ◽

Tatyana Yu. Rusanova ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pharmaceutical Preparation ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Animal Husbandry ◽

Biologically Active ◽

Natural Aluminosilicate ◽

Urgent Task ◽

Poultry Farming

Glauconite, a natural aluminosilicate, exhibits good sorption properties, is an affordable, cheap material, and has the prospect of being used in construction, ecology, medicine, cosmetology, animal husbandry, poultry farming, agriculture, etc. An urgent task is to study the sorption activity of glauconite in the Beloozersky deposit in the Saratov region in relation to biologically active substances for the creation of antimicrobial composites. The elemental composition and surface morphology of glauconite grains are determined. The sorption capacity of glauconite with respect to the pharmaceutical preparation rivanol based on acridine dye and the degree of its extraction from aqueous solutions were determined by spectrophotometry. A composite was obtained by sorption immobilization and analyzed for antimicrobial activity in relation to the standard strains of Staphylococcus aureus ATCC 6538 P and Escherichia coli ATCC 25922. An improvement in the antimicrobial properties of the immobilized preparation was revealed in comparison with an aqueous solution. The best effect of the composite in relation to Staphylococcus aureus ATCC 6538 P was determined compared to Escherichia coli ATCC 25922. The results of these studies can find practical application in veterinary medicine, poultry farming, agriculture, medicine, etc.

Download Full-text

АНТИМІКРОБНА АКТИВНІСТЬ ЕТАНОЛЬНОГО ЕКСТРАКТУ SERRATULA CORONATA L. (ASTERACEAE) ЗА ІНТРОДУКЦІЇ В ЖИТОМИРСЬКОМУ ПОЛІССІ

Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University ◽

10.15421/201616 ◽

2016 ◽

Vol 6 (01) ◽

pp. 290-303 ◽

Cited By ~ 1

Author(s):

I.V. Ivashchenko

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Antimicrobial Properties ◽

Ethanol Extract ◽

Antimicrobial Effect ◽

Minimal Bactericidal Concentration ◽

Lower Sensitivity ◽

Serratula Coronata

Antimicrobial properties of Serratula coronata L., introduced in Zhytomyr Polissya, were studied against test cultures of Escherichia coli (coliform bacillus) UCM B-906 (ATCC 25922), Staphylococcus aureus(golden staphylococcus) UCM B-904 (ATCC 25923), Pseudomonas aeruginosa (blue pus bacillus) UCM B-900 (ATCC9027), Candida albicans UCM Y-(ATCC 885-653).Serratula coronata L. 40 % ethanol extract showed potent antimicrobial effect against gram-positive bacteria Staphylococcus aureus. Compared with the solvent, MIC (minimal inhibitory concentration) and MBC (minimal bactericidal concentration) increased 32 and 8 times, respectively. The other microorganisms’ cultures demonstrated much lower sensitivity. The study revealed less significant effect of the extract against Candida albicans, Pseudomonas aeruginosa, and it failed to display any bactericidal/fungicidal effect against gram-negative strains of Escherichia coli, Pseudomonas aeruginosa and fungus Candida albicans.The data obtained experimentally show that further study of Serratula coronata L. and its application as the basis for new pharmaceutical anti-staphylococcus preparations may be considered promising and having a great potential.

Download Full-text