RAPID DETECTION OF OUTBREAK ESCHERICHIA COLI O157 AND SALMONELLA ON ALFALFA SPROUTS BY IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLUORESCENCE

2003 ◽  
Vol 23 (2) ◽  
pp. 75-89 ◽  
Author(s):  
SHU-I TU ◽  
MARSHA GOLDEN ◽  
WILLIAM F. FETT ◽  
ANDREW GEHRING ◽  
PETER IRWIN
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Escherichia Coli O157 ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Immunomagnetic Capture ◽  
Alfalfa Sprouts

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

Use of Spent Irrigation Water for Microbiological Analysis of Alfalfa Sprouts

2001 ◽  
Vol 64 (6) ◽  
pp. 802-806 ◽  
Author(s):  
T. FU ◽  
D. STEWART ◽  
K. REINEKE ◽  
J. ULASZEK ◽  
J. SCHLESSER ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Irrigation Water ◽  
Foodborne Illness ◽  
Microbiological Analysis ◽  
Escherichia Coli O157 ◽  
Salmonella Infection ◽  
Commercial Scale ◽  
Microbiological Testing ◽  
Rotary Drum ◽  
Alfalfa Sprouts

Numerous outbreaks of foodborne illness have been linked to the consumption of raw sprouts. Sprout producers have been advised by the Food and Drug Administration to include microbiological testing of spent irrigation water during production as part of an overall strategy to enhance the safety of sprouts. Alfalfa sprouts and irrigation water were analyzed to show the feasibility of using irrigation water for monitoring the microbiological safety of sprouts. Sprouts and water were produced and harvested from both commercial-scale (rotary drum) and consumer-scale (glass jars) equipment. Rapid increases of aerobic mesophiles occurred during the first 24 h of sprouting, with maximum levels achieved after 48 to 72 h. The counts in irrigation water were on average within approximately 1 log of their respective counts in the sprouts. Similar results were obtained for analysis of Escherichia coli O157:H7 in irrigation water and sprouts grown from artificially inoculated seeds. Testing of spent irrigation water indicated the contamination status of alfalfa sprouts grown from seeds associated with outbreaks of Salmonella infection.

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