Determination of Dose Rate of Florfenicol in Feed for Control of Mortality in Channel Catfish Ictalurus punctatus (Rafinesque) Infected with Edwardsiella ictaluri, Etiological Agent of Enteric Septicemia

2004 ◽  
Vol 35 (2) ◽  
pp. 257-267 ◽  
Author(s):  
Patricia S. Gaunt ◽  
Richard G. Endris ◽  
Lester Khoo ◽  
Rebecca Howard ◽  
Anissa L. McGinnis ◽  
...  
Keyword(s):  
Dose Rate ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Edwardsiella Ictaluri ◽  
Etiological Agent ◽  
Enteric Septicemia

scholarly journals Determination of Flumequine in Channel Catfish by Liquid Chromatography with Fluorescence Detection

1999 ◽  
Vol 82 (3) ◽  
pp. 614-619 ◽  
Author(s):  
Steven M Plakas ◽  
Kathleen R El Said ◽  
F Aladar Bencsath ◽  
Steven M Musser ◽  
Calvin C Walker
Keyword(s):  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Solid Phase ◽  
Signal To Noise Ratio ◽  
Methanol Solution ◽  
Signal To Noise ◽  
Relative Standard

Abstract Rapid methods are described for determination of flumequine (FLU) residues in muscle and plasma of farm-raised channel catfish (Ictalurus punctatus). FLU residues were extracted from tissues with an acidified methanol solution, and extracts were cleaned up on C18 solid-phase extraction cartridges. FLU concentrations were determined by liquid chromatography (LC)using a C18 analytical column and fluorescence detection (excitation, 325 nm; emission, 360 nm). Mean recoveries of FLU from fortified muscle were 87–94% at 5 levels ranging from 10 to 160 ppb (5 replicates per level). FLU recoveries from fortified plasma were 92–97% at 5 levels ranging from 20 to 320 ppb. Limits of detection (signal-to-noise ratio, 3:1)for the method as described were 3 and 6 ppb for muscle and plasma, respectively. Relative standard deviations (RSDs) for recoveries were ≤12%. Live catfish were dosed with 14C-labeled or unlabeled FLU to generate incurred residues. Recoveries of 14C residues throughout extraction and cleanup were 90 and 94% for muscle and plasma, respectively. RSDs for incurred FLU at 2 levels in muscle and plasma ranged from 2 to 6%. The identity of FLU in incurred tissues was confirmed by LC/mass spectrometry.

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