A MECHANISM FOR PROZONE FORMATION IN THE COMPLEMENT FIXATION TEST FOR BOVINE BRUCELLOSIS

1975 ◽  
Vol 51 (8) ◽  
pp. 374-377 ◽  
Author(s):  
P. Plackett ◽  
G. G. Alton
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Bovine Brucellosis

scholarly journals Comparison of radioimmunoassay with the complement fixation test and the indirect haemolysis test in the field diagnosis of bovine brucellosis

1983 ◽  
Vol 90 (1) ◽  
pp. 67-70 ◽  
Author(s):  
R. J. Chappel ◽  
J. Hayes
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Supporting Evidence ◽  
Bovine Brucellosis ◽  
Eradication Programme ◽  
Female Cattle ◽  
The Rose ◽  
Supplementary Test ◽  
Haemolysis Test

SUMMARYSera were collected from female cattle in 118 commercial herds being subjected to a programme to eradicate brucellosis by test and slaughter, in an area in which vaccination of heifer calves with Brucella abortus strain 19 was compulsory. Of 4583 sera positive by the Rose Bengal plate test, the brucellosis radioimmunoassay was positive for 1524, the complement fixation test for 1363 and the indirect haemolysis test for 1141. These figures, and supporting evidence from the eradication programme, suggest that the radioimmunoassay may be a useful supplementary test in problem herds.

scholarly journals Complement Fixation Test for Specific Antibody Detection against Bovine Brucellosis in Selected Peasant Association of Guto Gida District, East Wollega Zone, Oromia, Ethiopia

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Gudeta Ararsa ◽  
Eyob Hirpa ◽  
Morka Amante
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Animal Movement ◽  
Cross Sectional Study ◽  
Fixation Test ◽  
Control Measures ◽  
Antibody Detection ◽  
Fetal Membrane ◽  
Bovine Brucellosis ◽  
Cross Sectional

Brucellosis is one of the major zoonotic diseases yet prevalent in Ethiopia. It is contagious and has harmful effects on free animal movement and export. A cross-sectional study was conducted from November 2016 to April 2017 in the Loko and Uke peasant association of Guto Gida District. The goal of this study is to determine the seroprevalence and associated risk factors of bovine brucellosis. The questionnaire survey was conducted on 200 respondents to collect the livestock owner’s perception of this disease. Eighty (80) blood samples were collected from grazing cattle above six months of age. Serum was tested by complement fixation test (CFT) for Brucella antibody detection. Pearson chi-square is used to assess the relation of independent variables such as sex, site, and age with seroprevalence in a 95% confidence interval if P value is less than 0.05, recoded as significant. Seroprevalence of bovine brucellosis in the present study is 22.5%. Bovine brucellosis prevalence recorded in Uke (28.6%) was higher than that in Loko (21.2%). Again bovine brucellosis is higher in males (30.4%) than females (19.2%). There is prevalence variation among site, sex, and age which is statistically insignificant ( P value>0.05). Survey findings revealed that 52% (104/200) of respondents did not know the causes of abortion, while 48% (96/200) of them confirmed abortion in their herd and 64% (124/200) of respondents removed retained fetal membrane by themselves. Brucellosis is a contagious reproductive disease of cattle with zoonotic implications and needs to design and implement control measures aiming at preventing further spread of the disease.

scholarly journals Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis

1976 ◽  
Vol 77 (3) ◽  
pp. 369-376 ◽  
Author(s):  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught ◽  
M. J. Dalling ◽  
G. S. Allan
Keyword(s):  
Specific Antibody ◽  
Brucella Abortus ◽  
Bovine Serum ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Serological Test ◽  
Agglutination Test ◽  
Fixation Test ◽  
Bovine Brucellosis ◽  
Serum Agglutination

SUMMARYA radioimmunoassay (RIA) has been developed to measure antibodies against Brucella abortus in bovine serum and can be used in the diagnosis of bovine brucellosis. The RIA measures the amount of specific antibody of the IgG1 and IgG2 subclasses but is insensitive to 1gM, a characteristic which may make it more suitable than the complement fixation test (OFT) or the serum agglutination test for distinguishing infected animals from those which have been vaccinated with Br. abortus strain 19. The RIA is not subject to prozoning or ambiguous reactions, both of which interfere with the interpretation of the CFT.

Seroprevalence and risk factors of bovine brucellosis in the province of Djelfa (ALGERIA)

2018 ◽  
Author(s):  
A. Yahia ◽  
K. Hamrat ◽  
K. Saidani ◽  
R. Kaidi
Keyword(s):  
Risk Factors ◽  
Epidemiological Study ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Age Groups ◽  
Agglutination Test ◽  
Fixation Test ◽  
Bovine Brucellosis ◽  
Factors Affecting ◽  
Significant Difference

In order to determine the prevalence of bovine brucellosis and the factors affecting its persistence in the province of Djelfa (Algeria), a sero-epidemiological study was carried out on a total of 10827 cattle (7346 females and 3481 males) during the decade from 2004 to 2013. The sera were screened by card agglutination test, and the positive samples were confirmed by the complement fixation test. Some factors (year, age, breed and sex) were considered. Results found an average prevalence of brucellosis during these ten years of 1.4% with a highly significant difference according to year and sex (P Lass Than 0.05). A prevalence of 1.58% for females against a prevalence of 1.03% for males was observed. No significant difference was observed for the different age groups (P>0.05) in the seroprevalence of brucellosis. A higher prevalence of infection (1.53%) was found in imported breeds compared to local and cross breeds with (1.39%) and (1.35%) respectively. The effect of the breed is highly significant (P Lass Than 0.05). In conclusion the bovine brucellosis persists in the province of Djelfa and it is necessary to implement a scheme to fight and prevent this infection.

Evaluation of the Complement Fixation Test in Amebiasis

1952 ◽  
Vol 21 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Elwood Buchman ◽  
Harold J. Kullman ◽  
George F. Margonis
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test
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