Rapid in situ detection of virulent Vibrio vulnificus strains in raw oyster matrices using real-time PCR

2009 ◽  
Vol 2 (1) ◽  
pp. 76-80 ◽  
Author(s):  
Craig Baker-Austin ◽  
Anthony Gore ◽  
James D. Oliver ◽  
Rachel Rangdale ◽  
J Vaun McArthur ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Vibrio Vulnificus ◽  
In Situ Detection

scholarly journals Swine conjunctivitis outbreaks associated with Mycoplasma hyorhinis

2019 ◽  
Vol 31 (5) ◽  
pp. 766-769 ◽  
Author(s):  
Talita P. Resende ◽  
Maria Pieters ◽  
Fabio A. Vannucci
Keyword(s):  
In Situ Hybridization ◽  
Real Time ◽  
Real Time Pcr ◽  
Bacterial Load ◽  
Mycoplasma Hyorhinis ◽  
Swine Farms ◽  
High Bacterial Load ◽  
In Situ Detection ◽  
Swine Herds

Conjunctivitis is an uncommon finding in commercial swine herds, and the etiology of the disease is rarely studied. We investigated cases of conjunctivitis in 3 wean-to-finish swine farms. Eye swabs and tissues were obtained from clinically affected pigs (8–22 wk of age), from unaffected pigs in contact with affected pen-mates, and from age-matched pigs from an unaffected herd. Real-time PCR (rtPCR) testing for Mycoplasma hyorhinis demonstrated consistent detection and high bacterial load in samples from affected herds (clinically affected animals and non-clinical pen-mates). Ct values in affected pigs were 18.9–25.3; values were 36.4–38.6 in unaffected pigs from unaffected herds. Additionally, M. hyorhinis was identified within inflamed palpebral conjunctivae by in situ hybridization. The association of rtPCR and in situ detection of M. hyorhinis, along with the lack of detection of other potential pathogens and noninfectious causes, suggests the involvement of M. hyorhinis in the etiology and pathogenesis of the reported swine conjunctivitis.

Real-time in situ detection and source tracing of different soot

Optik ◽  
2021 ◽  
pp. 167711
Author(s):  
Enlai Wan ◽  
Zhongmou Sun ◽  
Yuzhu Liu
Keyword(s):  
Real Time ◽  
Source Tracing ◽  
In Situ Detection

Development of a real-time PCR assay for the detection of Trichinella spiralis in situ

2009 ◽  
Vol 161 (1-2) ◽  
pp. 92-98 ◽  
Author(s):  
H. Atterby ◽  
J. Learmount ◽  
C. Conyers ◽  
I. Zimmer ◽  
N. Boonham ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Trichinella Spiralis ◽  
Pcr Assay

scholarly journals Comparison of the sensitivity and specificity of real-time PCR and in situ hybridization in HPV16 and 18 detection in archival cervical cancer specimens

2012 ◽  
Vol 50 (2) ◽  
pp. 239-247 ◽  
Author(s):  
Beata Biesaga ◽  
Sława Szostek ◽  
Małgorzata Klimek ◽  
Jerzy Jakubowicz ◽  
Joanna Wysocka
Keyword(s):  
Cervical Cancer ◽  
Real Time ◽  
Sensitivity And Specificity ◽  
Real Time Pcr

scholarly journals Fluorophores from fungi

2003 ◽  
Vol 24 (3) ◽  
pp. 12 ◽  
Author(s):  
Duncan Veal ◽  
Philip Bell ◽  
Hayley Brown ◽  
Hung-Yoon Choi ◽  
Peter Karuso
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Dna Microarray ◽  
Differential Display ◽  
Real Time Pcr ◽  
In Situ Hybridisation ◽  
Analytical Techniques ◽  
Fluorescence In Situ Hybridisation

Fluorescence has many advantages over traditional colour and radioactive labels, and is playing an increasingly important role in the most powerful analytical techniques. For example, fluorescence is at the heart of many nucleic acid based diagnostics (e.g. DNA microarray, real time-PCR, fluorescence in situ hybridisation, etc), immunofluorescence assays, defined substrate technologies and differential display proteomics and is gradually replacing or complementing other techniques based on colour or radiolabels.

scholarly journals Real Time In-situ Detection System Research for Methane in Offshore Shallow Gas based on Thin Film Interface

2018 ◽  
Vol 232 ◽  
pp. 04053
Author(s):  
Cheng-xing Miao ◽  
Qing Li ◽  
Sheng-yao Jia
Keyword(s):  
Real Time ◽  
Detection Efficiency ◽  
Detection System ◽  
Detection Methods ◽  
Shallow Gas ◽  
Micro Gas Sensor ◽  
Detection Probe ◽  
On Line ◽  
In Situ Detection

In order to get ridded of the non real-time detection methods of artificial site sampled and laboratory instrument analyzed in the field of methane detection in the offshore shallow gas, real-time in-situ detection system for methane in offshore shallow gas was designed by the film interface.The methane in the offshore shallow gas through the gas-liquid separation membrane of polymer permeation into the system internal detection probe, analog infrared micro gas sensor sensed the methane concentration and the corresponded output value, data acquisition and communication node fitted into standard gas concentration.Based on the experimental data compared with the traditional detection method, and further analyzed the causes of error produced by the case experiment. The application results show that the system can achieve a single borehole layout, long-term on-line in-situ on-line detection, and improve the detection efficiency and the timeliness of the detection data.

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