scholarly journals Evaluation of microbial diversity in the pilot-scale beer brewing process by culture-dependent and culture-independent method

2015 ◽  
Vol 118 (2) ◽  
pp. 454-469 ◽  
Author(s):  
M. Takahashi ◽  
Y. Kita ◽  
K. Kusaka ◽  
A. Mizuno ◽  
N. Goto-Yamamoto
Keyword(s):  
Microbial Diversity ◽  
Pilot Scale ◽  
Independent Method ◽  
Beer Brewing ◽  
Culture Independent ◽  
Brewing Process ◽  
Culture Dependent

scholarly journals Evaluation of microbial diversity in the pilot-scale beer brewing process by culture-dependent and culture-independent method

2015 ◽  
Vol 119 (3) ◽  
pp. 904-904 ◽  
Author(s):  
M. Takahashi ◽  
Y. Kita ◽  
K. Kusaka ◽  
A. Mizuno ◽  
N. Goto-Yamamoto
Keyword(s):  
Microbial Diversity ◽  
Pilot Scale ◽  
Independent Method ◽  
Beer Brewing ◽  
Culture Independent ◽  
Brewing Process ◽  
Culture Dependent

scholarly journals Microbiota identified from preserved Anopheles

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Bianca E Silva ◽  
Zvifadzo Matsena Zingoni ◽  
Lizette L. Koekemoer ◽  
Yael L. Dahan-Moss
Keyword(s):  
Microbial Diversity ◽  
Vector Competence ◽  
Mosquito Species ◽  
Anopheles Funestus ◽  
Cost Effective ◽  
Diversity Indices ◽  
Malaria Vectors ◽  
Microbial Composition ◽  
Culture Independent ◽  
Culture Dependent

Abstract Background Mosquito species from the Anopheles gambiae complex and the Anopheles funestus group are dominant African malaria vectors. Mosquito microbiota play vital roles in physiology and vector competence. Recent research has focused on investigating the mosquito microbiota, especially in wild populations. Wild mosquitoes are preserved and transported to a laboratory for analyses. Thus far, microbial characterization post-preservation has been investigated in only Aedes vexans and Culex pipiens. Investigating the efficacy of cost-effective preservatives has also been limited to AllProtect reagent, ethanol and nucleic acid preservation buffer. This study characterized the microbiota of African Anopheles vectors: Anopheles arabiensis (member of the An. gambiae complex) and An. funestus (member of the An. funestus group), preserved on silica desiccant and RNAlater® solution. Methods Microbial composition and diversity were characterized using culture-dependent (midgut dissections, culturomics, MALDI-TOF MS) and culture-independent techniques (abdominal dissections, DNA extraction, next-generation sequencing) from laboratory (colonized) and field-collected mosquitoes. Colonized mosquitoes were either fresh (non-preserved) or preserved for 4 and 12 weeks on silica or in RNAlater®. Microbiota were also characterized from field-collected An. arabiensis preserved on silica for 8, 12 and 16 weeks. Results Elizabethkingia anophelis and Serratia oryzae were common between both vector species, while Enterobacter cloacae and Staphylococcus epidermidis were specific to females and males, respectively. Microbial diversity was not influenced by sex, condition (fresh or preserved), preservative, or preservation time-period; however, the type of bacterial identification technique affected all microbial diversity indices. Conclusions This study broadly characterized the microbiota of An. arabiensis and An. funestus. Silica- and RNAlater®-preservation were appropriate when paired with culture-dependent and culture-independent techniques, respectively. These results broaden the selection of cost-effective methods available for handling vector samples for downstream microbial analyses.

Comparison of culture-dependent and culture-independent techniques in the detection of lactic acid bacteria biodiversity and dynamics throughout the ripening process: The case of Turkish artisanal Tulum cheese produced in the Anamur region

2021 ◽  
pp. 1-7
Author(s):  
Talha Demirci ◽  
Aysun Oraç ◽  
Kübra Aktaş ◽  
Enes Dertli ◽  
Ismail Akyol ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Dna Isolation ◽  
Independent Method ◽  
Rrna Gene ◽  
Ripening Process ◽  
Culture Independent ◽  
Tulum Cheese ◽  
V3 Region ◽  
Culture Dependent ◽  
Bacterial 16S Rrna Gene

Abstract Our objective was to analyze the diversity of the microbiota over 180 d of ripening of eight batches of artisanal goatskin Tulum cheeses by culture-dependent and culture-independent (PCR-DGGE) methods. V3 region of the bacterial 16S rRNA gene was amplified with the PCR after direct DNA isolation from the cheese samples. Nine different species and five genera were determined by culturing, while 11 species were identified in the PCR-DGGE technique. This diversity revealed the uniqueness of artisanal cheese varieties. The dominant genera in all the cheese samples were composed of Enterococcus species. The culture-dependent method revealed five genera (Enterococcus,Bacillus,Lactococcus,Lactobacillus, Sphingomonas) while three genera (Enterococcus, Streptococcus, Lactococcus) were detected in the culture-independent method. It was concluded that combining the two methods is important for characterizing the whole microbiota of the Tulum cheese varieties produced in the Anamur region.

Assessment of Microbial Diversity in Saudi Springs by Culture-Dependent and Culture-Independent Methods

2016 ◽  
pp. 1-11
Author(s):  
Samy Selim ◽  
Sherif Hassan ◽  
Nashwa Hagagy ◽  
Lucia Kraková ◽  
Tomaš Grivalský ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
Culture Independent ◽  
Culture Dependent

Microbial diversity in sugarcane ethanol production in a Brazilian distillery using a culture-independent method

2014 ◽  
Vol 42 (1) ◽  
pp. 73-84 ◽  
Author(s):  
Ohana Yonara Assis Costa ◽  
Betulia Morais Souto ◽  
Daiva Domenech Tupinambá ◽  
Jessica Carvalho Bergmann ◽  
Cynthia Maria Kyaw ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
Ethanol Production ◽  
Independent Method ◽  
Culture Independent

Culture-dependent and culture-independent approaches to studying soil microbial diversity

2012 ◽  
Vol 32 (14) ◽  
pp. 4421-4433 ◽  
Author(s):  
刘国华 LIU Guohua ◽  
叶正芳 YE Zhengfang ◽  
吴为中 WU Weizhong
Keyword(s):  
Microbial Diversity ◽  
Soil Microbial Diversity ◽  
Approaches To Studying ◽  
Soil Microbial ◽  
Culture Independent ◽  
Culture Dependent

Culture-dependent and culture-independent approaches for studying airborne microbial diversity

2016 ◽  
Vol 36 (14) ◽  
Author(s):  
方治国 FANG Zhiguo ◽  
郝翠梅 HAO Cuimei ◽  
姚文冲 YAO Wenchong ◽  
欧阳志云 OUYANG Zhiyun
Keyword(s):  
Microbial Diversity ◽  
Culture Independent ◽  
Culture Dependent

scholarly journals Assessment of microbial diversity under arid plants by culture-dependent and culture-independent approaches

2013 ◽  
Vol 12 (40) ◽  
pp. 5860-5868
Author(s):  
Sharma Nimisha ◽  
Singh Govind ◽  
Sudarsan Yemmanuar
Keyword(s):  
Microbial Diversity ◽  
Culture Independent ◽  
Culture Dependent

scholarly journals Characterization of the microbial diversity in a permafrost sample from the Canadian high Arctic using culture-dependent and culture-independent methods

2007 ◽  
Vol 59 (2) ◽  
pp. 513-523 ◽  
Author(s):  
Blaire Steven ◽  
Geoffrey Briggs ◽  
Chris P. McKay ◽  
Wayne H. Pollard ◽  
Charles W. Greer ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
High Arctic ◽  
Canadian High Arctic ◽  
Culture Independent ◽  
Culture Dependent
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