Establishment and characterization of a cold‐sensitive neural cell line from the brain of tilapia ( Oreochromis niloticus )

2020 ◽  
Author(s):  
Yong Long ◽  
Ran Liu ◽  
Guili Song ◽  
Qing Li ◽  
Zongbin Cui
Keyword(s):  
Cell Line ◽  
Oreochromis Niloticus ◽  
Neural Cell ◽  
Cold Sensitive ◽  
The Brain

scholarly journals Characterization of mouse Ire1α: cloning, mRNA localization in the brain and functional analysis in a neural cell line

2000 ◽  
Vol 85 (1-2) ◽  
pp. 68-76 ◽  
Author(s):  
Ko Miyoshi ◽  
Taiichi Katayama ◽  
Kazunori Imaizumi ◽  
Manabu Taniguchi ◽  
Yasutake Mori ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Cell Line ◽  
Mrna Localization ◽  
Neural Cell ◽  
The Brain

Establishment and characterization of a novel cell line from the brain of golden pompano (Trachinotus ovatus)

2016 ◽  
Vol 52 (4) ◽  
pp. 410-418 ◽  
Author(s):  
Pengfei Li ◽  
Lingli Zhou ◽  
Songwei Ni ◽  
Meng Xu ◽  
Yepin Yu ◽  
...  
Keyword(s):  
Cell Line ◽  
Trachinotus Ovatus ◽  
Golden Pompano ◽  
The Brain

Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus

2015 ◽  
Vol 87 (1) ◽  
pp. 115-122 ◽  
Author(s):  
Y. Zheng ◽  
L. M. Peng ◽  
F. You ◽  
Y. X. Zou ◽  
P. J. Zhang ◽  
...  
Keyword(s):  
Cell Line ◽  
Paralichthys Olivaceus ◽  
Japanese Flounder ◽  
Fish Cell Line ◽  
Fish Cell ◽  
The Brain

Bilirubin-neural cell interaction: Characterization of initial cell surface binding leading to toxicity in the neuroblastoma cell line N-115

1990 ◽  
Vol 1055 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Yair Amit ◽  
Shirley Fedunec ◽  
Panakkezhum D. Thomas ◽  
Mark J. Poznansky ◽  
David Schiff
Keyword(s):  
Cell Surface ◽  
Cell Line ◽  
Neuroblastoma Cell ◽  
Cell Interaction ◽  
Neuroblastoma Cell Line ◽  
Neural Cell ◽  
Surface Binding ◽  
Initial Cell ◽  
Cell Surface Binding

Establishment and characterization of a cell line derived from the kidney of tilapia, Oreochromis niloticus

2019 ◽  
Vol 26 (2) ◽  
pp. 382
Author(s):  
Jianqing ZHAO ◽  
Peng JIA ◽  
Wenzhi LIU ◽  
Yong ZHOU ◽  
Nan JIANG ◽  
...  
Keyword(s):  
Cell Line ◽  
Oreochromis Niloticus ◽  
A Cell

Establishment and characterization of a continuous cell line from heart of Nile tilapia Oreochromis niloticus and its susceptibility to tilapia lake virus

2021 ◽  
Vol 287 ◽  
pp. 113989
Author(s):  
Manoj Kumar Yadav ◽  
Aakriti Rastogi ◽  
Mónica Paola Criollo Joaquin ◽  
Dev Kumar Verma ◽  
Gaurav Rathore ◽  
...  
Keyword(s):  
Cell Line ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Continuous Cell Line ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Glycogen phosphorylase isoenzymes from hepatoma 3924A and from a non-tumorigenic liver cell line. Comparison with the liver and brain enzymes

1992 ◽  
Vol 282 (3) ◽  
pp. 665-673 ◽  
Author(s):  
D Mayer ◽  
G Seelmann-Eggebert ◽  
I Letsch
Keyword(s):  
Cell Line ◽  
Liver Cell ◽  
Metabolic Regulation ◽  
Glycogen Phosphorylase ◽  
Main Band ◽  
Liver Cell Line ◽  
Normal Rat ◽  
Morris Hepatoma ◽  
The Brain

Glycogen phosphorylase isoenzymes were isolated from normal rat liver, rat brain, the glycogen-poor Morris hepatoma (MH) 3924A, and the glycogen-rich non-tumorigenic liver cell line C1I. Electrophoretic and immunological characterization of the enzymes showed that tumour and C1I cells expressed a phosphorylase isoform similar to the brain type; the liver type was not detectable. All enzymes were obtained as dimers; the Mr of the subunits was 96,000 (liver), 93,000 (brain and MH 3924A) and 92,000 (C1I). Isoelectric focusing revealed a main band of pI 6.34 for liver phosphorylase a, pI 5.67 for the enzymes from MH 3924A and brain, and pI 5.68 for C1I phosphorylase. Partial kinetic characterization of the AMP-independent forms of the isoenzymes yielded Km values for glucose 1-phosphate of 3.5 +/- 0.5 mM (liver), 3.9 mM (brain), 1.9 +/- 0.3 mM (MH 3924A) and 2.5 +/- 0.5 mM (C1I); Km values for glycogen were 0.4 mM (liver) and 0.3 mM (MH 3924A and C1I), calculated as glucose equivalents. The AMP-independent phosphorylase was inhibited by glucose 6-phosphate (Glc6P) with Ki values of 0.32 +/- 0.03 mM (C1I), 0.50 +/- 0.04 mM (MH 3924A) and approximately 5 mM (brain). The inhibition could be abolished by 1 mM-AMP, indicating that AMP and Glc6P may partially compete for the same site on the protein. Liver phosphorylase a was not inhibited by up to 25 mM-Glc6P. In contrast with liver and brain isoenzymes, phosphorylase from the cell lines was not affected by NaF and Na2SO4. The data show that both the hepatocellular carcinoma and the non-malignant immortalized liver cells express a phosphorylase isoform different from the liver type. Furthermore, there is some evidence that the enzyme from MH 3924A and C1I cells is distinct from brain phosphorylase a, in spite of electrophoretic and immunological resemblance, and that this isoenzyme is subject to altered metabolic regulation.

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