Digestive proteinases from marine fish processing wastes of Southwest Atlantic Ocean: their partial characterization and comparison

2021 ◽  
Author(s):  
Ivana Soledad Friedman ◽  
Leonel Agustín Behrens ◽  
Nair de los Angeles Pereira ◽  
Edgardo Martín Contreras ◽  
Analia Verónica Fernández‐Gimenez
2020 ◽  
Author(s):  
Ivana Soledad Friedman ◽  
Leonel Agustín Behrens ◽  
Nair A Pereira ◽  
Edgardo Contreras ◽  
Analia Verónica Fernández-Gimenez

Abstract Fish processing generates a lot of wastes which are discarded resulting in environmental problems. However, this material represents a significant source of high-value bioproducts with potential biotechnological applications. The objective of this study was to characterize and to compare specific activities of acid and alkaline proteases recovered from the viscera of Merluccius hubbsi (Mh), Percophis brasiliensis (Pb), Urophyis brasiliensis (Ub), and Cynoscion guatucupa (Cg) under different pH and temperature conditions. Stomach proteinases from four species had a higher activity at pH 2, with stability in the range of pH 2-4. Optimum pH from intestinal enzymes of Cg was 11.5, while for the crude extract of Mh, Pb, and Ub catalytic activity was registered over a wide pH range range from 7 to 11.5. Stomach proteinases from four studied species had a higher activity at 30 °C and 50 °C, with stability at 10 °C and 30 °C. Optimum temperature from intestinal enzymes of the four tested species was 50 °C with high stability at 10 °C and 30 °C. Alkaline proteinase from all species and acid proteinases from Cg was inactivated at 70ºC, while stomach enzymes of Mh, Pb, and Ub had a residual activity lower than 5% at 80 °C after 5, 10 y 20 minutes of pre-incubation, respectively. Digestive proteinases recovered in this study could be used as biocatalysts in industrial processes, reducing costs, adding value to the fishery waste, and contributing to the reduction of environmental pollution.


2020 ◽  
Vol 2 (1) ◽  
pp. 90

Marine fish processing industries located in the urban region involves fish processing activities that generate a considerable amount of leftover biological residues such as solid wastes and protein-rich effluents, which are ineffectively discarded in water bodies and landfills affecting the coastal environment. The present study involves the biological treatment of industrial fish processing wastes through the co-composting method to convert the fish wastes into organic input. Fish wastes were mixed with rice husk as a bulking agent and allowed for organic matter stabilization through the co-composting process. The maturity of the co-compost was evaluated by spectroscopic techniques, nutrient analysis, and seed germination assay. UV-vis spectroscopic analysis revealed the presence of absorbance between 250 and 300 nm indicating the characteristics of increased concentration of aromatic compounds. The humification index (E4/E6) was calculated to be 1.2, which signified the characteristics of humified materials. Conventional fluorescence spectroscopic analysis detected emission spectra centered at 448 nm due to the development of aromatic structures as a result of increasing the humification process in the co-compost. The well-composted organic matter was found to contain macronutrients (N-1.35%; P-1.02%; K-0.51%) and micronutrients (Zn-74.07 mg/kg; Cu-16.37 mg/kg; Fe-176.07 mg/kg) respectively. Further, seed germination sassay showed growth promotion effects of the co-compost in seeds of Vigna radiata; Vigna mungo and Trigonella foenum-graecum, which confirmed the stability of the organic matter. The findings of the study conclude that the co-composting process of fish industrial processing wastes using rice husk could offer effective conversion of fish waste into organic co-compost for soil application.


2020 ◽  
Vol 30 (3) ◽  
pp. 426-439 ◽  
Author(s):  
Laura Schejter ◽  
Gabriel Genzano ◽  
Esteban Gaitán ◽  
Carlos D. Perez ◽  
Claudia S. Bremec

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
M. Sofía Dutto ◽  
Gabriel N. Genzano ◽  
Agustín Schiariti ◽  
Julieta Lecanda ◽  
Mónica S. Hoffmeyer ◽  
...  

Author(s):  
Tracey Anne Colley ◽  
Judith Valerian ◽  
Michael Zwicky Hauschild ◽  
Stig Irving Olsen ◽  
Morten Birkved

Nutrient depletion in Tanzanian sisal production has led to yield decreases over time. We use nutrient mass balances embedded within a life cycle assessment to quantify the extent of nutrient depletion for different production systems, then used circular economy principles to identify potential cosubstrates from within the Tanzanian economy to anaerobically digest with sisal wastes. The biogas produced is then used to generate bioelectricity and the digestate residual can be used as a fertilizer to address the nutrient depletion. If no current beneficial use of the cosubstrate was assumed, then beef manure and marine fish processing waste were the best cosubstrates. If agricultural wastes were assumed to have a current beneficial use as fertilizer, then marine fish processing waste and human urine were the best cosubstrates. The largest reduction in environmental impacts resulted from bioelectricity replacing electricity from fossil fuels in the national electricity grid and improved onsite waste management practices. There is significant potential to revitalize Tanzanian sisal production by applying circular economy principles to sisal waste management and bioenergy production.


Author(s):  
Paulo Júnior ◽  
Christiane de Souza ◽  
Graciela Weiss

Composition of Trichiuridae and Gempylidae larvae (Teleostei) and their association with water masses in the Southwest Atlantic OceanIn this paper the relationship between temporal changes in the occurrence of water masses and Trichiuridae and Gempylidae larvae composition and distribution in the Southwest Atlantic ocean were analysed between 25° and 40° S. Ichthyoplankton was collected during the three expeditions of the Subtropical Convergence Project: Winter and Spring 1977, Autumn 1978 and Summer 1981, realized in the Southwest Atlantic waters. Oblique tows were conducted using a Hensen net with 250 μm mesh size. Steep salinity and temperature gradient were found, where the river outflows from La Plata river (Argentina) and Patos Lagoon (Brazil) met the Tropical Water over the continental shelf between 32 and 36° S. We examined 524 Hensen-net samples that contained about 283 larvae from five species of Trichiuridae and Gempylidae. The most abundant and frequent specie were


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