Ultrastructural insights into the replication cycle of salmon pancreas disease virus (SPDV) using salmon cardiac primary cultures (SCPCs)

2021 ◽  
Author(s):  
Patricia Noguera ◽  
Matthias Klinger ◽  
Histro Örün ◽  
Bianka Grunow ◽  
Jorge del‐Pozo
Keyword(s):  
Disease Virus ◽  
Primary Cultures ◽  
Replication Cycle ◽  
Pancreas Disease ◽  
Salmon Pancreas Disease Virus

scholarly journals Comparison of Two Aquatic Alphaviruses, Salmon Pancreas Disease Virus and Sleeping Disease Virus, by Using Genome Sequence Analysis, Monoclonal Reactivity, and Cross-Infection

2002 ◽  
Vol 76 (12) ◽  
pp. 6155-6163 ◽  
Author(s):  
Jonathan Weston ◽  
Stéphane Villoing ◽  
Michel Brémont ◽  
Jeanette Castric ◽  
Martin Pfeffer ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Amino Acid ◽  
Atlantic Salmon ◽  
Disease Virus ◽  
Antigenic Variation ◽  
Encephalitis Virus ◽  
Virus Species ◽  
Pancreas Disease ◽  
Equine Encephalitis Virus ◽  
Salmon Pancreas Disease Virus

ABSTRACT Cell culture isolates of salmon pancreas disease virus (SPDV) of farmed Atlantic salmon and sleeping disease virus (SDV) of rainbow trout were compared. Excluding the poly(A) tracts, the genomic nucleotide sequences of SPDV and SDV RNAs include 11,919 and 11,900 nucleotides, respectively. Phylogenetic analysis places SPDV and SDV between the New World viruses of Venezuelan equine encephalitis virus and Eastern equine encephalitis virus and the Old World viruses of Aura virus and Sindbis virus. When compared to each other, SPDV and SDV show 91.1% nucleotide sequence identity over their complete genomes, with 95 and 93.6% amino acid identities over their nonstructural and structural proteins, respectively. Notable differences between the two viruses include a 24-nucleotide insertion in the C terminus of nsP3 protein of SPDV and amino acid sequence variation at the C termini of the capsid and E1 proteins. Experimental infections of Atlantic salmon and rainbow trout with SPDV and SDV confirmed that the disease lesions induced by SPDV and SDV were similar in nature. Although infections with SPDV and SDV produced similar levels of histopathology in rainbow trout, SDV induced significantly less severe lesions in salmon than did SPDV. Virus neutralization tests performed with sera from experimentally infected salmon indicated that SPDV and SDV belonged to the same serotype; however, antigenic variation was detected among SDV and geographically different SPDV isolates by using monoclonal antibodies. Although SPDV and SDV exhibit minor biological differences, we conclude on the basis of the close genetic similarity that SPDV and SDV are closely related isolates of the same virus species for which the name Salmonid alphavirus is proposed.

Isolation of salmon pancreas disease virus (SPDV) in cell culture and its ability to protect against infection by the ‘wild-type’ agent

2001 ◽  
Vol 11 (6) ◽  
pp. 505-522 ◽  
Author(s):  
M.V. López-Dóriga ◽  
D.A. Smail ◽  
R.J. Smith ◽  
A. Doménech ◽  
J. Castric ◽  
...  
Keyword(s):  
Cell Culture ◽  
Disease Virus ◽  
Wild Type ◽  
Pancreas Disease ◽  
Salmon Pancreas Disease Virus

Isolation of salmon pancreas disease virus (SPDV) from farmed Atlantic salmon, Salmo salar L., in Scotland

1998 ◽  
Vol 21 (6) ◽  
pp. 469-472 ◽  
Author(s):  
H. M. Rowley ◽  
C. E. Doherty ◽  
M. F. McLoughlin ◽  
M. D. Welsh
Keyword(s):  
Atlantic Salmon ◽  
Disease Virus ◽  
Salmo Salar ◽  
Pancreas Disease ◽  
Atlantic Salmon Salmo Salar ◽  
Farmed Atlantic Salmon ◽  
Salmon Pancreas Disease Virus

scholarly journals Experimental pancreas disease in Atlantic salmon Salmo salar post-smolts induced by salmon pancreas disease virus (SPDV)

1996 ◽  
Vol 26 ◽  
pp. 117-124 ◽  
Author(s):  
MF McLoughlin ◽  
RT Nelson ◽  
HM Rowley ◽  
DI Cox ◽  
AN Grant
Keyword(s):  
Atlantic Salmon ◽  
Disease Virus ◽  
Salmo Salar ◽  
Pancreas Disease ◽  
Atlantic Salmon Salmo Salar ◽  
Salmon Pancreas Disease Virus

scholarly journals Production and characterisation of monoclonal antibodies to salmon pancreas disease virus

2001 ◽  
Vol 46 ◽  
pp. 101-108 ◽  
Author(s):  
D Todd ◽  
VA Jewhurst ◽  
MD Welsh ◽  
BJ Borghmans ◽  
JH Weston ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Disease Virus ◽  
Pancreas Disease ◽  
Salmon Pancreas Disease Virus

scholarly journals Biochemical characterization of salmon pancreas disease virus

2000 ◽  
Vol 81 (3) ◽  
pp. 813-820 ◽  
Author(s):  
Michael Welsh ◽  
Marian McLoughlin ◽  
Jonathan Weston ◽  
Daniel Todd ◽  
Helen Rowley ◽  
...  
Keyword(s):  
Disease Virus ◽  
Biochemical Characterization ◽  
Pancreas Disease ◽  
Salmon Pancreas Disease Virus
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