Rapid visual detection of Micropterus salmoides rhabdovirus using recombinase polymerase amplification combined with lateral flow dipsticks

2022 ◽  
Author(s):  
Zizhao Feng ◽  
Xin Chu ◽  
Minzhen Han ◽  
Chenwei Yu ◽  
Yousheng Jiang ◽  
...  
Keyword(s):  
Visual Detection ◽  
Micropterus Salmoides ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification

Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymerase amplification combined with a lateral flow dipstick

2018 ◽  
Vol 41 (8) ◽  
pp. 1201-1206 ◽  
Author(s):  
H Wang ◽  
M Sun ◽  
D Xu ◽  
P Podok ◽  
J Xie ◽  
...  
Keyword(s):  
Visual Detection ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Lateral Flow Dipstick

Development of recombinase polymerase amplification combined with lateral flow detection assay for rapid and visual detection of Ralstonia solanacearum in tobacco

Plant Disease ◽  
2021 ◽  
Author(s):  
Changfeng Li ◽  
Yuliang Ju ◽  
Xun Wu ◽  
Pengfei Shen ◽  
Le Cao ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Visual Detection ◽  
High Sensitivity ◽  
High Specificity ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Molecular Diagnostic ◽  
Tobacco Stem ◽  
Detection Assay ◽  
Flow Detection

Bacterial wilt caused by Ralstonia solanacearum is a serious soil-borne disease that results in severe losses to tobacco (Nicotiana tabacum) production in China. In this study, a novel RPA-LFD assay for the rapid visual detection of R. solanacearum was established using recombinase polymerase amplification (RPA) and lateral-flow dipstick (LFD). The RPA-LFD assay was performed at 37°C in 30 min without complex equipment. Targeting the sequence of the RipTALI-9 gene, we designed RPA primers (Rs-rpa-F/R) and an LF probe (Rs-LF-probe) that showed high specificity to R. solanacearum. The sensitivity of RPA-LFD assay to R. solanacearum was the same as that in conventional PCR at 1 pg genomic DNA, 102 CFU/g artificially inoculated tobacco stem, and 103 CFU/g artificially inoculated soil. The RPA-LFD assay could also detect R. solanacearum from plant and soil samples collected from naturally infested tobacco fields. These results suggest that the RPA-LFD assay developed in this study is a rapid, accurate molecular diagnostic tool with high sensitivity for the detection of R. solanacearum.

Rapid and visual detection of enterovirus using recombinase polymerase amplification combined with lateral flow strips

2020 ◽  
Vol 311 ◽  
pp. 127903 ◽  
Author(s):  
Xiaohan Yang ◽  
Jia Xie ◽  
Siqi Hu ◽  
Wenli Zhan ◽  
Lei Duan ◽  
...  
Keyword(s):  
Visual Detection ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Lateral Flow Strips

Rapid and visual detection of Verticillium dahliae using recombinase polymerase amplification combined with lateral flow dipstick

2020 ◽  
Vol 136 ◽  
pp. 105226 ◽  
Author(s):  
Yuliang Ju ◽  
Changfeng Li ◽  
Pengfei Shen ◽  
Ning Wan ◽  
Wenbing Han ◽  
...  
Keyword(s):  
Verticillium Dahliae ◽  
Visual Detection ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Lateral Flow Dipstick
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