Inhibition of PaCaMKII-E isoform in the dorsal unpaired median neurosecretory cells of cockroach reduces nicotine- and clothianidin-induced currents

2014 ◽  
Vol 130 (4) ◽  
pp. 507-513 ◽  
Author(s):  
Olivier List ◽  
Delphine Calas-List ◽  
Emiliane Taillebois ◽  
Marjorie Juchaux ◽  
Emilie Heuland ◽  
...  
Keyword(s):  
Neurosecretory Cells ◽  
Induced Currents ◽  
Median Neurosecretory Cells ◽  
Dorsal Unpaired Median

Co-localization of amines and peptides in the same median neurosecretory cells of locusts

1982 ◽  
Vol 31 (3) ◽  
pp. 237-240 ◽  
Author(s):  
J. Viellemaringe ◽  
P. Duris ◽  
C. Bensch ◽  
J. Girardie
Keyword(s):  
Neurosecretory Cells ◽  
Median Neurosecretory Cells

Two distinct low-voltage-activated Ca2+ currents contribute to the pacemaker mechanism in cockroach dorsal unpaired median neurons

1996 ◽  
Vol 76 (2) ◽  
pp. 963-976 ◽  
Author(s):  
F. Grolleau ◽  
B. Lapied
Keyword(s):  
Voltage Dependence ◽  
Low Voltage ◽  
Neurosecretory Cells ◽  
Pacemaker Activity ◽  
Tetraacetic Acid ◽  
Current Clamp ◽  
Patch Clamp Technique ◽  
Time To Peak ◽  
Whole Cell Patch Clamp ◽  
Dorsal Unpaired Median

1. The contribution of Ca2+ currents to the endogenous firing properties of cockroach isolated adult dorsal unpaired median neurons was investigated using whole cell patch-clamp technique with 5 mM Ca2+ as the charge carrier. At least three types of Ca2+ currents, a high-voltage-activated Ca2+ current and two low-voltage-activated (LVA) Ca2+ currents, have been found in these neurons. This study focused on the LVA Ca2+ currents, which are suitable candidates in the generation of the slow predepolarization because of their low threshold of activation. 2. The global LVA Ca2+ current could be dissociated by means of nickel sensitivity, deactivation time constant and voltage dependence of time to peak, tail current amplitude and inactivation, as transient and maintained LVA Ca2+ currents. 3. The transient LVA Ca2+ current, sensitive to 100 microM Ni2+, was isolated by using a subtraction procedure. It was activated at -70 mV and half-inactivated at -59.5 mV. The inactivation was purely voltage dependent. Current-clamp experiments performed with 150 microM Ni2+ indicated that this current was involved in the initial part of the predepolarization. 4. The maintained LVA Ca2+ current, resistant to 100 microM Ni2+, was activated in a range of potential 10 mV more positive than the transient LVA Ca2+ current, and its voltage dependence of inactivation displayed a U-shaped-curve. 5. Replacing Ca2+ with Ba2+ in equimolar amount or low internal Ca2+ concentration [5 mM bis-(o-aminophenoxy)-N,N,N',N'-tetraacetic acid (BAPTA) in the pipette] induced a monotonic voltage dependence of inactivation and increased the rate of relaxation of this current. These effects were mimicked by high internal Ca2+ concentration [0.1 mM Ca2+ and no ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid in the pipette]. This demonstrated an unusual Ca2+-sensitive inactivation process that varied over a narrow range of Ca2+ concentrations. 6. Current-clamp experiments performed under 150 microM Ni2+, with 15 mM external Ca2+ concentration (which potentiated the maintained LVA current within 30 s of superfusion) or with 5 mM BAPTA in the pipette demonstrated the participation of this current in the last two-thirds of the slow predepolarizing phase. 7. Our findings demonstrated, for the first time in neurosecretory cells, the coexistence of two distinct LVA Ca2+ currents, which have specialized function in the generation of the pacemaker activity.

The Relationships Between Nutrition, Hormones and Reproduction in the Blowfly Calliphora Erythrocephala (Meig.)

1961 ◽  
Vol 38 (3) ◽  
pp. 637-646
Author(s):  
J. STRANGWAYS-DIXON
Keyword(s):  
Gravid Female ◽  
Neurosecretory Cells ◽  
Volume Activity ◽  
Calliphora Erythrocephala ◽  
Carbohydrate Consumption ◽  
Low Protein ◽  
Low Carbohydrate ◽  
Protein Selection ◽  
Median Neurosecretory Cells ◽  
Reproductive Cycles

1. A study has been made of the effects of removal of ovaries, copora allata (c.a.) and median neurosecretory cells (m.n.c.) upon the selection by female blowflies of carbohydrate (sugar in water) or ‘protein’ (Marmite in milk). 2. Extirpation of the ovaries resulted in high carbohydrate-low protein selection and in hypertrophy of the c.a. 3. Extirpation of the c.a. resulted in low carbohydrate selection. 4. Extirpation of both ovaries and c.a. resulted in low carbohydrate selection. 5. These and other results suggest that selection is independent of direct ovarian control, but that the ovaries influence selection in that they utilize the ingested foods and thus bring about the succession of feeding cycles. 6. The c.a., whose volume (activity?) changes cyclically during each cycle of reproduction, appears to control the fluctuations in carbohydrate consumption. 7. The m.n.c. seem to be necessary for the ingestion of ‘protein’ and for the activity of the c.a. 8. Reproductive cycles tend to overlap. The succeeding cycle in a gravid female must be taken into consideration when events are being related to reproduction.

Ovulation in Rhodnius prolixus Stal is induced by an extract of neurosecretory cells

1983 ◽  
Vol 61 (3) ◽  
pp. 684-686 ◽  
Author(s):  
F. L. Kriger ◽  
K. G. Davey
Keyword(s):  
Neurosecretory Cells ◽  
Rhodnius Prolixus ◽  
Pars Intercerebralis ◽  
Median Neurosecretory Cells ◽  
The Brain

The injection of an extract of 10 identified median neurosecretory cells from the pars intercerebralis into gravid mated females previously deprived of these cells by surgery induces ovulation and oviposition during the ensuring 24 h. Injection of an extract of ocellar nerves has no effect. These observations support the hypothesis that ovulation and oviposition are controlled by a myotropin released from neurosecretory cells in the brain.

Role of the Median Neurosecretory Cells in the Ovarian Development of the Blow Fly Protophormia terraenovae

1999 ◽  
Vol 16 (2) ◽  
pp. 187-191 ◽  
Author(s):  
Ikuyo Toyoda ◽  
Hideharu Numata ◽  
Sakiko Shiga
Keyword(s):  
Ovarian Development ◽  
Neurosecretory Cells ◽  
Blow Fly ◽  
Median Neurosecretory Cells

scholarly journals DILP-producing median neurosecretory cells in the Drosophila brain mediate the response of lifespan to nutrition

Aging Cell ◽  
2010 ◽  
Vol 9 (3) ◽  
pp. 336-346 ◽  
Author(s):  
Susan J. Broughton ◽  
Cathy Slack ◽  
Nazif Alic ◽  
Athanasios Metaxakis ◽  
Timothy M. Bass ◽  
...  
Keyword(s):  
Neurosecretory Cells ◽  
Drosophila Brain ◽  
Median Neurosecretory Cells

scholarly journals Insulin signalling regulates remating in female Drosophila

2010 ◽  
Vol 278 (1704) ◽  
pp. 424-431 ◽  
Author(s):  
Stuart Wigby ◽  
Cathy Slack ◽  
Sebastian Grönke ◽  
Pedro Martinez ◽  
Federico C. F. Calboli ◽  
...  
Keyword(s):  
Fat Body ◽  
Insulin Signalling ◽  
Underlying Mechanism ◽  
Neurosecretory Cells ◽  
Dominant Negative ◽  
Developmental Defects ◽  
Mating Rate ◽  
Female Remating ◽  
Reduced Body ◽  
Median Neurosecretory Cells

Mating rate is a major determinant of female lifespan and fitness, and is predicted to optimize at an intermediate level, beyond which superfluous matings are costly. In female Drosophila melanogaster , nutrition is a key regulator of mating rate but the underlying mechanism is unknown. The evolutionarily conserved insulin/insulin-like growth factor-like signalling (IIS) pathway is responsive to nutrition, and regulates development, metabolism, stress resistance, fecundity and lifespan. Here we show that inhibition of IIS, by ablation of Drosophila insulin-like peptide (DILP)-producing median neurosecretory cells, knockout of dilp2 , dilp3 or dilp5 genes, expression of a dominant-negative DILP-receptor ( InR ) transgene or knockout of Lnk , results in reduced female remating rates. IIS-mediated regulation of female remating can occur independent of virgin receptivity, developmental defects, reduced body size or fecundity, and the receipt of the female receptivity-inhibiting male sex peptide. Our results provide a likely mechanism by which females match remating rates to the perceived nutritional environment. The findings suggest that longevity-mediating genes could often have pleiotropic effects on remating rate. However, overexpression of the IIS-regulated transcription factor dFOXO in the fat body—which extends lifespan—does not affect remating rate. Thus, long life and reduced remating are not obligatorily coupled.

Morphology and histology of the endocrine glands of Zootermopsis angusticollis Hagen (Isoptera)

1972 ◽  
Vol 50 (12) ◽  
pp. 1537-1547 ◽  
Author(s):  
Cedric Gillott ◽  
Chih-Ming Yin
Keyword(s):  
Cell Types ◽  
Cell Number ◽  
Neurosecretory Cells ◽  
Corpora Allata ◽  
Endocrine Glands ◽  
Histological Changes ◽  
Secretory Cycle ◽  
Zootermopsis Angusticollis ◽  
Primary Reproductives ◽  
Median Neurosecretory Cells

The morphology and histology of the endocrine glands of the various castes of Zootermopsis angmticollis were examined. Six types of median neurosecretory cells are distinguishable by size and stain affinity. No differences in the relative numbers of these cell types among castes can be seen. It is postulated that they may be different forms of the same cell during its synthetic and secretory cycle. Lateral neurosecretory cells can be identified consistently only in mature primary reproductives of both sexes. In the corpora cardiaca two types of cells occur; the fuchsinophilic cells are distributed generally whereas the cells that take up counterstain are restricted to the center of the gland. Intercellular neurosecretory cell product is found throughout the gland. No histological changes associated with the formation of particular castes were observed. The corpora allata (CA) vary in size and histological appearance according to caste but not sex. The CA of reproductives (primary and supplementary) and presoldiers are larger than those of juveniles of the same instar; those of soldiers are about the same size as those of the corresponding juvenile stage. These size increases are due mainly to changes in the ratio cytoplasmic diameter: nuclear diameter of the constituent cells and not to an increase in cell number. The H-shaped molt glands, which, as in other pterygote insects, disappear within a few days of the imaginal molt, are composed of a large prothoracic portion and a smaller cephalic ('ventral gland') portion extending anteriorly and dorsally. Histologically the two components are indistinguishable. Running through the glands are thin strands of muscle.

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