Genetic and Molecular Mapping of Stripe Rust Resistance Genes in Wheat Cultivar Zhongliang 12

Stripe rust, caused by the fungus Puccinia striiformis f. sp. tritici, is a worldwide disease of wheat that causes devastating crop losses. Resistant cultivars have been developed over the last 40 years that have significantly reduced the economic impact of the disease on growers, but in heavy infection years it is mostly controlled through the intensive application of fungicides. The Pacific Northwest of the United States has an ideal climate for stripe rust and has one of the most diverse race compositions in the country. This has resulted in many waves of epidemics that have overcome most of the resistance genes traditionally used in elite germplasm. The best way to prevent high yield losses, reduce production costs to growers, and reduce the heavy application of fungicides is to pyramid multiple stripe rust resistance genes into new cultivars. Using genotyping-by-sequencing, we identified 4662 high quality variant positions in a recombinant inbred line population of 196 individuals derived from a cross between Skiles, a highly resistant winter wheat cultivar, and Goetze, a moderately to highly susceptible winter wheat cultivar, both developed at Oregon State University. A subsequent genome wide association study identified two quantitative trait loci (QTL) on chromosomes 3B and 3D within the predicted locations of stripe rust resistance genes. Resistance QTL, when combined together, conferred high levels of stripe rust resistance above the level of Skiles in some locations, indicating that these QTL would be important additions to future breeding efforts of Pacific Northwest winter wheat cultivars.

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Characterization of Wheat Stripe Rust Resistance Genes in Shaanmai 139

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2010.00109 ◽

2010 ◽

Vol 36 (1) ◽

pp. 109-114 ◽

Cited By ~ 8

Author(s):

Hong ZHANG ◽

Zhi-Long REN ◽

Yin-Gang HU ◽

Chang-You WANG ◽

Wan-Quan JI

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Wheat Stripe Rust ◽

Rust Resistance Genes

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Allelic analysis of stripe rust resistance genes on wheat chromosome 2BS

Genome ◽

10.1139/g08-079 ◽

2008 ◽

Vol 51 (11) ◽

pp. 922-927 ◽

Cited By ~ 35

Author(s):

P. G. Luo ◽

X. Y. Hu ◽

Z. L. Ren ◽

H. Y. Zhang ◽

K. Shu ◽

...

Keyword(s):

Ssr Markers ◽

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Dominant Gene ◽

Wheat Chromosome ◽

Stripe Rust Resistance ◽

Resistance Response ◽

Rust Resistance Genes ◽

Yr Genes

Stripe rust, caused by Puccinia striiormis Westend f. sp. tritici, is one of the most important foliar diseases of wheat ( Triticum aestivum L.) worldwide. Stripe rust resistance genes Yr27, Yr31, YrSp, YrV23, and YrCN19 on chromosome 2BS confer resistance to some or all Chinese P. striiormis f. sp. tritici races CYR31, CYR32, SY11-4, and SY11-14 in the greenhouse. To screen microsatellite (SSR) markers linked with YrCN19, F1, F2, and F3 populations derived from cross Ch377/CN19 were screened with race CYR32 and 35 SSR primer pairs. Linkage analysis indicated that the single dominant gene YrCN19 in cultivar CN19 was linked with SSR markers Xgwm410, Xgwm374, Xwmc477, and Xgwm382 on chromosome 2BS with genetic distances of 0.3, 7.9, 12.3, and 21.2 cM, respectively. Crosses of CN19 with wheat lines carrying other genes on chromosome 2B showed that all were located at different loci. YrCN19 is thus different from the other reported Yr genes in chromosomal location and resistance response and was therefore named Yr41. Prospects and strategies of using Yr41 and other Yr genes in wheat improvement for stripe rust resistance are discussed.

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Molecular Marker Based Design for Breeding Wheat Lines with Multiple Resistance and Superior Quality

Plant Disease ◽

10.1094/pdis-02-20-0420-re ◽

2020 ◽

Vol 104 (10) ◽

pp. 2658-2664

Author(s):

Tao Liu ◽

George Fedak ◽

Lianquan Zhang ◽

Rangrang Zhou ◽

Dawn Chi ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

High Resistance ◽

Stem Rust ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Multiple Resistance ◽

Wheat Stem Rust ◽

Wheat Production ◽

Rust Resistance Genes

There has not been a major wheat stem rust epidemic worldwide since the 1970s, but the emergence of race TTKSK of Puccinia graminis f. sp. tritici in 1998 presented a great threat to the world wheat production. Single disease-resistance genes are usually effective for only several years before the pathogen changes genetically to overcome the resistance. Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is one of the most common and persistent wheat diseases worldwide. The development of varieties with multiple resistance is the most economical and effective strategy for preventing stripe rust and stem rust, the two main rust diseases constraining wheat production. Plateau 448 has been widely used in the spring wheat growing region in northwest China, but it has become susceptible to stripe rust and is susceptible to TTKSK. To produce more durable resistance to race TTKSK as well as to stripe rust, four stem rust resistance genes (Sr33, Sr36, Sr-Cad, and Sr43) and three stripe rust resistance genes (Yr5, Yr18, and Yr26) were simultaneously introgressed into Plateau 448 to improve its stem rust (Ug99) and stripe rust resistance using a marker-assisted backcrossing strategy combined with phenotypic selection. We obtained 131 BC1F5 lines that pyramided two to four Ug99 resistance genes and one to two Pst resistance genes simultaneously. Thirteen of these lines were selected for their TTKSK resistance, and all of them exhibited near immunity or high resistance to TTKSK. Among the 131 pyramided lines, 95 showed high resistance to mixed Pst races. Nine lines exhibited not only high resistance to TTKSK and Pst but also better agronomic traits and high-molecular-weight glutenin subunit compositions than Plateau 448.

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