Author response for "Assessment of genetic diversity among soybean genotypes differing in response to aerial blight (Rhizoctonia solaniKuhn) using SSR markers"

2020 ◽  
Author(s):  
Surbhi, Kumari ◽  
Singh, Krishna P. ◽  
Singh, Narendra K. ◽  
Aravind, T.
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Author Response ◽  
Soybean Genotypes

scholarly journals Validation of Drought Tolerance Gene-linked Microsatellite Markers and Their Efficiency for Diversity Assessment in a Set of Soybean Genotypes

2021 ◽  
pp. 48-57
Author(s):  
Nishi Mishra ◽  
M. K. Tripathi ◽  
Niraj Tripathi ◽  
Sushma Tiwari ◽  
Neha Gupta ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Drought Tolerance ◽  
Ssr Markers ◽  
Diversity Analysis ◽  
Molecular Screening ◽  
Genetic Diversity Analysis ◽  
Drought Tolerant ◽  
Diversity Assessment ◽  
Soybean Genotypes ◽  
Tolerance Gene

Aim: Soybean is well-thought-out to be a major crop owing to its significant involvement as vegetable oil and protein in human diet. However, inopportunely, its production has been melodramatically declined attributable to the commonness of drought related stress. Study Design: During the present study a total of 53 soybean genotypes were selected. For molecular diversity analysis as well as validation total 12 SSR markers were used. Molecular screening of soybean genotypes was done to determine the efficiency of available markers in genetic diversity analysis as well as their validation on the basis of their association with drought tolerance gene. Place and Duration of the Study: The present study was conducted at Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Gwalior, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M.P., India during the year 2018 - 2019. Methodology: Template DNA of all 53 selected soybean genotypes extracted for molecular screening. The current investigation has been accomplished to validate the available SSR markers with their efficiency in genetic diversity analysis in a set of soybean genotypes. Results: Among applied drought tolerance gene-linked 12 SSR molecular markers, the highest genetic diversity (0.6629) was noticed in Satt520 while lowest (0.0370) was in Satt557 with an average of 0. 3746.While, the highest PIC value was 0.5887 prearranged by Satt520 and lowest 0.0363 by Satt557 with the mean worth of 0.3063. Conclusion: Dendrogram constructed on the basis of banding profile of employed markers was able to discriminate some putative drought tolerant genotypes i.e., JS97-52, JS95-60 from rest of the genotypes. The results of the present examination may donate towards enhancement of soybean genotypes to bread drought tolerant varieties.

scholarly journals Genetic Diversity Analysis of Soybean [Glycine max (L.) Merrill.] Germplasms in Bangladesh Using SSR Markers

2021 ◽  
Vol 13 (2) ◽  
pp. 1
Author(s):  
M. K. Khatun ◽  
M. A. Haque ◽  
M. A. Malek ◽  
M. H. Rashid ◽  
S. Islam ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Polymorphic Information Content ◽  
Mean Value ◽  
Ammonium Bromide ◽  
Diversity Analysis ◽  
Dna Ladder ◽  
Genetic Diversity Analysis ◽  
Soybean Genotypes ◽  
The Mean

The purpose of the present study was to investigate the genetic diversity and molecular characterization of 50 soybean genotypes in Bangladesh with 20 SSR markers. Genomic DNA was isolated by modified standard cetyl tri-methyl ammonium bromide (CTAB) extraction protocol and alleles were amplified by polymerase chain reaction (PCR). Allele sizes were estimated in comparison with 50 bp DNA ladder. The software NTSYSpc version 2.2 and POPGENE version 1.31 were utilized for molecular data analysis and preparation of dendrogram. Polymorphic Information Content (PIC) values varied from 0.53 (Satt664) to 0.98 (Satt009, Satt330 and Satt522) with the mean value 0.897 and expected heterozygosity varied from 0.4059 (Satt685) to 0.1246 (Satt664) with the mean value 0.244. The dendrogram analysis depicted that the 50 genotypes were grouped in four (4) major clusters. The most diverse genotypes were SBG-1, PM-78-6-3-13, BS-3 and AGS-31, which suggest that the simple sequence repeat (SSR) markers are very efficient for genetic diversity analysis. The similarity matrix revealed the diversity among genotypes. The diverse genetic materials obtained from the present study on genetic diversity of soybean genotypes in Bangladesh may be utilized in the future breeding programme.

scholarly journals Genetic diversity of soybean genotypes different in powdery mildew resistance using SSR markers

2017 ◽  
Vol 33 (1S) ◽  
Author(s):  
Doan Thi Thuy Linh ◽  
Tran Thi Truong ◽  
Chu Hoang Ha ◽  
Le Van Son
Keyword(s):  
Genetic Diversity ◽  
Powdery Mildew ◽  
Ssr Markers ◽  
Genetic Basis ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Soybean Genotypes ◽  
Group 2 ◽  
Simple Sequence ◽  
Group 1

Powdery mildew disease, one of the major diseases of soybean in Vietnam was caused by Microsphaera diffusa. SSR (Simple sequence repeats) marker, because of their co-dominance, polymorphic and stability have been wildely used to measure genetic diversity in crop. In the present study, genetic basis of 36 soybean genotypes varying in powdery mildew resistance was studied using 14 SSR markers. A total of 61 alleles with an average of 4,36 allele/locus, which indicated hight genetic diversity of the studied genotypes. PIC value of each marker ranged from 0 (Sat_396, Satt183, Sat_298) to 0,711 (Sat_224) with an average of 0.364 which shown high genetic diverrsity among the studied genotypes. At genetic similarity coefficient of 0.75, 36 soybean genotypes divided into 8 distint groups. Group 1 and group 2 included 21/25 genotypes which was resistant to powdery mildew. Groups 1, 2, 4, 5 and 8 include powdery mildew resistance genotypes.  

Genetic diversity analysis among soybean genotypes using SSR markers in Uganda

2020 ◽  
Vol 19 (7) ◽  
pp. 439-448
Author(s):  
Mukuze Clever ◽  
Tukamuhabwa Phinehas ◽  
Maphosa Mcebisi ◽  
Dari Shorai ◽  
Onziga Dramadri Isaac ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Soybean Genotypes

scholarly journals Genetic diversity analysis of soybean (Glycine max (L.) Merr.) genotypes making use of SSR markers

2019 ◽  
pp. 1113-1119
Author(s):  
Keitumetse Kujane ◽  
Moosa M Sedibe ◽  
Alina Mofokeng
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Polymorphic Information Content ◽  
Molecular Data ◽  
Dna Ladder ◽  
Ssr Primers ◽  
Soybean Genotypes ◽  
Glycine Max L ◽  
Simple Sequence ◽  
Selection Of

In this study, we aimed to investigate the genetic diversity and polymorphism among 30 soybean genotypes maintained by the ARC using simple sequence repeat (SSR) markers. Soybean genotypes were characterized using 20 SSR primers. DNA was extracted using the standard cetyl trimethylammonium bromide method and amplified using PCR. Allele size was determined via comparison with a 100 base pair (bp) DNA ladder. Molecular data were analyzed, and a dendrogram and matrix were generated using GGT 2.0 software. A total of 216 alleles with an average of 10.8 alleles per locus were detected. The allele sizes ranged between 2 and 33 bp with an average of 18.7 bp. The polymorphic information content among genotypes varied from 0.85 (Satt001) to 0.75 (Satt43) with an average of 0.716, and heterozygosity ranged from 0.87 to 0.78 with an average of 0.7485. The most diverse genotypes were B 66 S 31, 69S 7, and R5-4-2 M, which indicated the efficiency of the SSR markers for the detection of genetic diversity. The results of the current study revealed the diversity among the soybean genotypes tested, which might aid breeders in the future in the selection of parents for breeding.

scholarly journals Genetic diversity analysis of soybean genotypes using SSR markers for salinity tolerance

2019 ◽  
Vol 30 (1) ◽  
pp. 1-9
Author(s):  
M Moniruzzaman ◽  
RM Saiem ◽  
RM Emon ◽  
MS Haque ◽  
NR Saha ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Ssr Markers ◽  
Gene Diversity ◽  
Dry Weight ◽  
Null Alleles ◽  
Root Weight ◽  
Root And Shoot Length ◽  
Soybean Genotypes ◽  
Ssr Loci

Soil salinity is a major constraint to soybean production. Five soybean genotypes were grown in pots with hydroponic culture under control and different salt stressed conditions to observe salt tolerance capacity on the basis of phenotypic screening and measure genetic diversity and relatedness among the genotypes. Minimum effects of salinity on root and shoot length was observed in Binasoyben-3, GC840 and Binasoyben-5at different salt stresses. Root dry weight and shoot dry weight of different soybean genotypes under different salt stresses were depicted. The highest reduction in root weight was noted in Binasoybean-1. The same genotypes were used to assess genetic diversity among them with simple sequence repeat (SSR) markers. A total of 33 alleles were detected among 5 soybean genotypes by using 10SSR markers. The number of alleles per locus ranged from 2 to 5, with an average of 3.33 alleles across the 10 loci. Rare alleles were observed at 10 SSR loci with an average of 2.8 alleles per locus. In this experiment, two SSR loci were found to be null alleles. The average values of null allele were 0.2. PIC values ranged from 0.27 in Satt184 to 0.77 in Satt339 with the average value of 0.56.The major allele frequency of the most common allele at each locus ranged from 0.80 in Satt184 to 0.20 in Satt339with a mean frequency of 0.48. The size of the different major alleles at different loci ranged from 173 bp for Satt509 to 407 bp for Satt339. The highest gene diversity (0.80) was observed in loci Satt339 and the lowest gene diversity (0.32) was observed in loci Satt184 with the mean diversity of 0.61. The lowest genetic distance (0.60) was observed in Asset vs Binasoybean-3 and Binasoybean-5 vs Binasoybean-3. The highest genetic distance (1.0) was observed between a numbers of genotype pairs with GC840 vs Asset. The UPGMA cluster analysis led to the grouping of the 5 genotypes into two major clusters. GC840, an advanced line identified to be salt tolerant, together with Binasoybean-5 and Binasoybean-3 clustered in the same sub group. The results from morphological and molecular study suggested that GC840 and Binasoybean-3 are moderately tolerant to salt stress. Progressive Agriculture 30 (1): 1-9, 2019

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