Lettuce growers in coastal California have relied mainly on protective fungicide sprays to control downy mildew. Thus, timing of sprays before infection is critical for optimal results. A leaf-wetness-driven, infection-based advisory system, previously developed, did not always perform satisfactorily. In this study, the advisory system was modified by incorporating a pathogen survival component (system 1) or both survival and sporulation components (system 2). These systems were then evaluated in commercial lettuce fields in coastal California during 1996-1998. Three or four treatments were carried out in each field: (i) no spray; (ii) sprays as scheduled by the growers; (iii) sprays following modified system 1; and (iv) sprays following the original advisory system (1996) or modified system 2 (1998). Downy mildew incidence was evaluated every 2 to 9 days. In fields with drip irrigation, the number of fungicide applications was reduced by one or two regardless of the advisory system used compared to the grower's calendar-based schedule, although one unnecessary spray was recommended in 1996 at Soledad and 1997 at Salinas. Under all three systems, disease levels were low (incidence <25% and about 1 lesion per plant) for fields with drip irrigation, but not for fields with sprinklers (incidence up to 100% and 5 to 10 lesions per plant). For the first time, we established that survival and sporulation components are not needed for a lettuce downy mildew forecasting system. Instead, a threshold with a shorter period of morning leaf wetness and high temperatures were found to have potential for improving forecasting efficiency.
Recognition of lettuce downy mildew effector BLR38 in Lactuca serriola
LS102 requires two unlinked loci