scholarly journals A YajQ‐LysR‐like, cyclic di‐GMP‐dependent system regulating biosynthesis of an antifungal antibiotic in a crop‐protecting bacterium, Lysobacter enzymogenes

2019 ◽  
Vol 21 (2) ◽  
pp. 218-229 ◽  
Author(s):  
Sen Han ◽  
Danyu Shen ◽  
Yu‐Chuan Wang ◽  
Shan‐Ho Chou ◽  
Mark Gomelsky ◽  
...  
Keyword(s):  
Lysobacter Enzymogenes ◽  
Antifungal Antibiotic

scholarly journals Coordination of cyclic di-GMP and 4-hydroxybenzoic acid in regulating antifungal antibiotic biosynthesis in Lysobacter enzymogenes

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Sen Han ◽  
Mingming Yang ◽  
Alex M. Fulano ◽  
Long Lin ◽  
Shan-Ho Chou ◽  
...  
Keyword(s):  
Coordination Mechanism ◽  
Upstream Region ◽  
Antibiotic Biosynthesis ◽  
Hydroxybenzoic Acid ◽  
Lysobacter Enzymogenes ◽  
Antifungal Antibiotic ◽  
Cellular Processes ◽  
Heat Stable ◽  
Operon Expression ◽  
Shed Light

Abstract Small molecules are able to regulate numerous cellular processes through binding to various bacterial receptor proteins, but the mechanisms and functions by which these chemicals coordinate and execute remain poorly understood. 4-hydroxybenzoic acid (4-HBA) and cyclic di-GMP (c-di-GMP) are two such molecules with distinct structures that are produced in Lysobacter enzymogenes to synergistically affect the secretion of an antifungal antibiotic, known as heat-stable antifungal factor (HSAF). In our earlier studies, we showed that CdgL, a YajQ-like protein without DNA-binding domain, was able to physically interact with LysR, a transcription factor, to enhance its binding affinity toward the upstream region of the HSAF biosynthesis operon promoter, hence increasing the HSAF biosynthesis. Interestingly, 4-HBA or c-di-GMP can bind to its cognate receptor of LysR or CdgL, respectively, to regulate the HSAF biosynthesis. Further, c-di-GMP acts by binding to CdgL to induce the dissociation of the CdgL-LysR complex, leading to decreased downstream expression. We now showed that CdgL controlled the transcription of lenB2, which encodes an oxygenase to convert chorismate to 4-HBA. Notably, overexpression of cdgL was found to stimulate lenB2 transcription, which likely increased the intracellular 4-HBA content. Also, 4-HBA could bind to LysR to interrupt the LysR-CdgL complex formation and release of CdgL, which caused a lower affinity of LysR toward DNA and hence decreased HSAF operon expression. These findings, along with our earlier report, allow us to propose a coordination mechanism demonstrating how the HSAF biosynthesis is co-regulated by 4-HBA and c-di-GMP through interactions with their cognate receptors. This new mechanism shall shed light on improving the HSAF yield for practical usage.

scholarly journals Facile Method for Site-specific Gene Integration in Lysobacter enzymogenes for Yield Improvement of the Anti-MRSA Antibiotics WAP-8294A and the Antifungal Antibiotic HSAF

2013 ◽  
Vol 2 (11) ◽  
pp. 670-678 ◽  
Author(s):  
Yan Wang ◽  
Guoliang Qian ◽  
Fengquan Liu ◽  
Yue-Zhong Li ◽  
Yuemao Shen ◽  
...  
Keyword(s):  
Specific Gene ◽  
Yield Improvement ◽  
Site Specific ◽  
Lysobacter Enzymogenes ◽  
Antifungal Antibiotic ◽  
Gene Integration

scholarly journals Lysobacter PilR, the Regulator of Type IV Pilus Synthesis, Controls Antifungal Antibiotic Production via a Cyclic di-GMP Pathway

2017 ◽  
Vol 83 (7) ◽  
Author(s):  
Yuan Chen ◽  
Jing Xia ◽  
Zhenhe Su ◽  
Gaoge Xu ◽  
Mark Gomelsky ◽  
...  
Keyword(s):  
Response Regulator ◽  
Antibiotic Production ◽  
Second Messenger ◽  
Twitching Motility ◽  
Type Iv Pilus ◽  
Content Type ◽  
Lysobacter Enzymogenes ◽  
Antifungal Antibiotic ◽  
Type Iv ◽  
Two Component

ABSTRACT Lysobacter enzymogenes is a ubiquitous soil gammaproteobacterium that produces a broad-spectrum antifungal antibiotic, known as heat-stable antifungal factor (HSAF). To increase HSAF production for use against fungal crop diseases, it is important to understand how HSAF synthesis is regulated. To gain insights into transcriptional regulation of the HSAF synthesis gene cluster, we generated a library with deletion mutations in the genes predicted to encode response regulators of the two-component signaling systems in L. enzymogenes strain OH11. By quantifying HSAF production levels in the 45 constructed mutants, we identified two strains that produced significantly smaller amounts of HSAF. One of the mutations affected a gene encoding a conserved bacterial response regulator, PilR, which is commonly associated with type IV pilus synthesis. We determined that L. enzymogenes PilR regulates pilus synthesis and twitching motility via a traditional pathway, by binding to the pilA promoter and upregulating pilA expression. Regulation of HSAF production by PilR was found to be independent of pilus formation. We discovered that the pilR mutant contained significantly higher intracellular levels of the second messenger cyclic di-GMP (c-di-GMP) and that this was the inhibitory signal for HSAF production. Therefore, the type IV pilus regulator PilR in L. enzymogenes activates twitching motility while downregulating antibiotic HSAF production by increasing intracellular c-di-GMP levels. This study identifies a new role of a common pilus regulator in proteobacteria and provides guidance for increasing antifungal antibiotic production in L. enzymogenes. IMPORTANCE PilR is a widespread response regulator of the two-component system known for regulating type IV pilus synthesis in proteobacteria. Here we report that, in the soil bacterium Lysobacter enzymogenes, PilR regulates pilus synthesis and twitching motility, as expected. Unexpectedly, PilR was also found to control intracellular levels of the second messenger c-di-GMP, which in turn inhibits production of the antifungal antibiotic HSAF. The coordinated production of type IV pili and antifungal antibiotics has not been observed previously.

Exemplar Abstract for Lysobacter enzymogenes Christensen and Cook 1978 (Approved Lists 1980) emend. Lin et al. 2016.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Lysobacter Enzymogenes

Exemplar Abstract for Lysobacter enzymogenes cookii Christensen and Cook 1978.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Sarah Wigley ◽  
George M Garrity ◽  
Dorothea Taylor
Keyword(s):  
Lysobacter Enzymogenes

Nomenclature Abstract for Lysobacter enzymogenes Christensen and Cook 1978 (Approved Lists 1980) emend. Lin et al. 2016.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Sarah Wigley ◽  
George M Garrity
Keyword(s):  
Lysobacter Enzymogenes

Nomenclature Abstract for Lysobacter enzymogenes cookii Christensen and Cook 1978.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity
Keyword(s):  
Lysobacter Enzymogenes

Nomenclature Abstract for Lysobacter enzymogenes enzymogenes Christensen and Cook 1978.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity
Keyword(s):  
Lysobacter Enzymogenes

scholarly journals KB425796-A, a novel antifungal antibiotic produced by Paenibacillus sp. 530603

2013 ◽  
Vol 66 (8) ◽  
pp. 465-471 ◽  
Author(s):  
Hirohito Kai ◽  
Midori Yamashita ◽  
Shigehiro Takase ◽  
Michizane Hashimoto ◽  
Hideyuki Muramatsu ◽  
...  
Keyword(s):  
Antifungal Antibiotic ◽  
Paenibacillus Sp

Semi-synthesis and biological evaluation of analogues of UK-2A, a novel antifungal antibiotic from Streptomyces sp. 517-02

2005 ◽  
Vol 15 (8) ◽  
pp. 2011-2014 ◽  
Author(s):  
Yoshinosuke Usuki ◽  
Koichi Mitomo ◽  
Noriko Adachi ◽  
Xu Ping ◽  
Ken-Ichi Fujita ◽  
...  
Keyword(s):  
Biological Evaluation ◽  
Streptomyces Sp ◽  
Antifungal Antibiotic ◽  
Synthesis And Biological Evaluation
Sign in / Sign up

Export Citation Format

Share Document