Mapping and candidate gene screening of Cladosporium fulvum resistance gene Cf‐12 in tomato ( Solanum lycopersicum ) by high‐throughput sequencing

2020 ◽  
Vol 139 (5) ◽  
pp. 977-987
Author(s):  
Xinfeng Chai ◽  
Xiangyang XU ◽  
Dong Wang ◽  
Dongqi Xue ◽  
Jingfu Li
Keyword(s):  
Candidate Gene ◽  
Solanum Lycopersicum ◽  
Resistance Gene ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Cladosporium Fulvum ◽  
Gene Screening

scholarly journals A Mutagenesis Assay for Reporter Gene Screening Using Partially Degenerate Oligonucleotides of the Tandems NNT and NNC

2015 ◽  
Vol 2015 ◽  
pp. 1-7
Author(s):  
Huifen Xu ◽  
Cuilan Zhou ◽  
Andy K. Zhang ◽  
Wen Li ◽  
Jia Zhang ◽  
...  
Keyword(s):  
Pharmaceutical Industry ◽  
Candidate Gene ◽  
Resistance Gene ◽  
Reporter Gene ◽  
Specific Protein ◽  
High Tolerance ◽  
Nonsense Mutations ◽  
Gene Screening ◽  
Zeocin Resistance

Not all proteins are tolerable to mutations. Whether a specific protein can be a mutable target is of importance in the biotechnology and pharmaceutical industry. This study reported a novel mutagenesis assay using tandem NNT and NNC oligonucleotides to test the mutability of a candidate gene. These two tandem oligonucleotides avoid the risk of forming nonsense mutations and render flexibility of truncating or expanding the insertion size. As a reporter gene, ZeoR (zeocin resistance gene) was confirmed to have a high tolerance for mutagenesis by this new assay.

scholarly journals Identification of Stress Associated microRNAs in Solanum lycopersicum by High-Throughput Sequencing

Genes ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 475 ◽  
Author(s):  
López-Galiano ◽  
Sentandreu ◽  
Martínez-Ramírez ◽  
Rausell ◽  
Real ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Solanum Lycopersicum ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Expression Profiles ◽  
Plant Defence ◽  
Stress Conditions ◽  
Differentially Expressed ◽  
Physiological Adaptation ◽  
Biotic And Abiotic Stress

Tomato (Solanum lycopersicum) is one of the most important crops around the world and also a model plant to study response to stress. High-throughput sequencing was used to analyse the microRNA (miRNA) profile of tomato plants undergoing five biotic and abiotic stress conditions (drought, heat, P. syringae infection, B. cinerea infection, and herbivore insect attack with Leptinotarsa decemlineata larvae) and one chemical treatment with a plant defence inducer, hexanoic acid. We identified 104 conserved miRNAs belonging to 37 families and we predicted 61 novel tomato miRNAs. Among those 165 miRNAs, 41 were stress-responsive. Reverse transcription quantitative PCR (RT-qPCR) was used to validate high-throughput expression analysis data, confirming the expression profiles of 10 out of 11 randomly selected miRNAs. Most of the differentially expressed miRNAs were stress-specific, except for sly-miR167c-3p upregulated in B. cinerea and P. syringae infection, sly-newmiR26-3p upregulated in drought and Hx treatment samples, and sly-newmiR33-3p, sly-newmiR6-3p and sly-newmiR8-3p differentially expressed both in biotic and abiotic stresses. From mature miRNAs sequences of the 41 stress-responsive miRNAs 279 targets were predicted. An inverse correlation between the expression profiles of 4 selected miRNAs (sly-miR171a, sly-miR172c, sly-newmiR22-3p and sly-miR167c-3p) and their target genes (Kinesin, PPR, GRAS40, ABC transporter, GDP and RLP1) was confirmed by RT-qPCR. Altogether, our analysis of miRNAs in different biotic and abiotic stress conditions highlight the interest to understand the functional role of miRNAs in tomato stress response as well as their putative targets which could help to elucidate plants molecular and physiological adaptation to stress.

Fine mapping and candidate gene screening of the downy mildew resistance gene RPF1 in Spinach

2018 ◽  
Vol 131 (12) ◽  
pp. 2529-2541 ◽  
Author(s):  
Hongbing She ◽  
Wei Qian ◽  
Helong Zhang ◽  
Zhiyuan Liu ◽  
Xiaowu Wang ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Downy Mildew ◽  
Resistance Gene ◽  
Fine Mapping ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Gene Screening
Sign in / Sign up

Export Citation Format

Share Document