Plastidial and cytosolic thiol reductases participate in the control of stomatal functioning

Abaxial epidermal strips from leaves of Paphiopedilum leeanum were analyzed via sodium cobaltinitrite staining and atomic absorption spectrophotometry for the presence and location of potassium. On a dry weight basis K content of the abaxial epidermis was found to be 103 times less than has been reported in other species, and unlike other species no localization of K+ in guard cells of open stomata could be detected via the sodium cobaltinitrite stain for potassium.Flame photometric analysis of the mesophyll indicated that it contained normal amounts of K+ (about 1.87% on a dry weight basis). Analysis showed that the K+ content of the abaxial epidermis (0.032%) was considerably less than that of the mesophyll, a situation unlike previous reports for other species in which the epidermal concentration was found to be greater than the mesophyll. A process for exclusion of K+ from the abaxial epidermis is suggested, as is the lack of involvement of K+ as the major osmoticum in the stomatal mechanism of this species.

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Natural variation in stomatal response to closing stimuli among Arabidopsis thaliana accessions after exposure to low VPD as a tool to recognize the mechanism of disturbed stomatal functioning

Journal of Experimental Botany ◽

10.1093/jxb/eru370 ◽

2014 ◽

Vol 65 (22) ◽

pp. 6529-6542 ◽

Cited By ~ 40

Author(s):

Sasan Aliniaeifard ◽

Uulke van Meeteren

Keyword(s):

Arabidopsis Thaliana ◽

Natural Variation ◽

Stomatal Response ◽

Stomatal Functioning

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Improving stomatal functioning at elevated growth air humidity: A review

Journal of Plant Physiology ◽

10.1016/j.jplph.2016.10.003 ◽

2016 ◽

Vol 207 ◽

pp. 51-60 ◽

Cited By ~ 35

Author(s):

Dimitrios Fanourakis ◽

Dimitrios Bouranis ◽

Habtamu Giday ◽

Dália R.A. Carvalho ◽

Abdolhossein Rezaei Nejad ◽

...

Keyword(s):

Air Humidity ◽

Stomatal Functioning

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A Model for Simulating Transpiration of Leaves with Special Attention to Stomatal Functioning

Journal of Applied Ecology ◽

10.2307/2402046 ◽

1972 ◽

Vol 9 (1) ◽

pp. 57 ◽

Cited By ~ 23

Author(s):

F. W. T. Penning de Vries

Keyword(s):

Stomatal Functioning

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The occurrence of functional non-chlorophyllous guard cells in Paphiopedilum spp.

Canadian Journal of Botany ◽

10.1139/b75-001 ◽

1975 ◽

Vol 53 (1) ◽

pp. 1-7 ◽

Cited By ~ 46

Author(s):

Sherman D. Nelson ◽

James M. Mayo

Keyword(s):

Fluorescence Microscopy ◽

Blue Light ◽

Guard Cell ◽

Direct Evidence ◽

Light Response ◽

Guard Cells ◽

Epidermal Cells ◽

Stomatal Response ◽

Intact Leaves ◽

Stomatal Functioning

Hypostomatous lady slipper orchids, Paphiopedilum spp., were found to have non-chlorophyllous epidermal cells, including guard cells. The lack of chlorophyll within the guard cells was demonstrated by fluorescence microscopy. A "normal" chlorophyllous mesophyll was present. The leaf resistances of intact leaves were about 5–10 s cm−1 in the light and were greater than 100 s cm−1 in the dark, indicating light opening and dark closure of the stomata. A CO2-dependent stomatal response (i.e., a tendency to close at elevated CO2 levels) was demonstrated, as was a CO2-independent light response (i.e., greater opening in blue light than in red). This provides direct evidence to support the idea that guard cell chlorophyll is not necessary for stomatal functioning.

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