The soybean β‐expansin gene GmINS1 contributes to nodule development in response to phosphate starvation

2021 ◽  
Author(s):  
Zhaojun Yang ◽  
Jiakun Zheng ◽  
Huiwen Zhou ◽  
Shengnan Chen ◽  
Zhi Gao ◽  
...  
Keyword(s):  
Phosphate Starvation ◽  
Nodule Development ◽  
Expansin Gene

scholarly journals The Expression of MaEXP1, a Melilotus alba Expansin Gene, Is Upregulated During the Sweetclover-Sinorhizobium meliloti Interaction

2004 ◽  
Vol 17 (6) ◽  
pp. 613-622 ◽  
Author(s):  
Walter Giordano ◽  
Ann M. Hirsch
Keyword(s):  
Cell Wall ◽  
Sinorhizobium Meliloti ◽  
Plant Cell Wall ◽  
Nodule Development ◽  
Northern Analysis ◽  
Degenerate Primers ◽  
Melilotus Alba ◽  
Expansin Gene ◽  
Rapid Amplification

Expansins are a highly conserved group of cell wall-localized proteins that appear to mediate changes in cell wall plasticity during cell expansion or differentiation. The accumulation of expansin protein or the mRNA for specific expansin gene family members has been correlated with the growth of various plant organs. Because expansin proteins are closely associated with plant cell wall expansion, and as part of a larger study to determine the role of different gene products in the legume-Rhizobium spp. symbiosis, we investigated whether a Melilotus alba (white sweetclover) expansin gene is expressed during nodule development. A cDNA fragment encoding an expansin gene (EXP) was isolated from Sinorhizobium meliloti-inoculated sweetclover root RNA by reverse-transcriptase polymerase chain reaction using degenerate primers, and a full-length sweetclover expansin sequence (MaEXP1) was obtained using 5′ and 3′rapid amplification of cDNA end cloning. The predicted amino acid of the sweetclover expansin is highly conserved with the various α-expansins in the GenBank database. MaEXP1 contains a series of eight cysteines and four tryptophans that are conserved in the α-expansin protein family. Northern analysis and whole-mount in situ hybridization analyses indicate that MaEXP1 mRNA expression is enhanced in roots within hours after inoculation with S. meliloti and in nodules. Western and immunolocalization studies using a cucumber expansin antibody demonstrated that a cross-reacting protein accumulated in the expanding cells of the nodule.

NEGATIVE HORMONAL REGULATION OF SYMBIOTIC NODULE DEVELOPMENT. II. SALICILIC, JASMONIC AND ABSCISIC ACIDS (review)

2018 ◽  
Vol 53 (1) ◽  
pp. 3-14 ◽  
Author(s):  
A.V. Tsyganova ◽  
◽  
V.E Tsyganov ◽  
Keyword(s):  
Hormonal Regulation ◽  
Nodule Development ◽  
Symbiotic Nodule
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