Antisperm antibodies in repeat-breeding cows: Frequency, detection and validation of threshold levels employing sperm immobilization, sperm agglutination and immunoperoxidase assay

2016 ◽  
Vol 52 (2) ◽  
pp. 195-202 ◽  
Author(s):  
SK Srivastava ◽  
S Shinde ◽  
SK Singh ◽  
S Mehrotra ◽  
MR Verma ◽  
...  
Keyword(s):  
Antisperm Antibodies ◽  
Threshold Levels ◽  
Frequency Detection ◽  
Immunoperoxidase Assay ◽  
Sperm Immobilization

scholarly journals Human sperm carbohydrate antigens defined by an antisperm human monoclonal antibody derived from an infertile woman bearing antisperm antibodies in her serum.

1988 ◽  
Vol 168 (1) ◽  
pp. 343-356 ◽  
Author(s):  
Y Tsuji ◽  
H Clausen ◽  
E Nudelman ◽  
T Kaizu ◽  
S Hakomori ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Monoclonal Antibody ◽  
Human Sperm ◽  
Infertile Woman ◽  
Antisperm Antibodies ◽  
Infertile Women ◽  
Epitope Structure ◽  
Sperm Immobilization ◽  
Sperm Antigen

The epitope structure of the human sperm antigen reacting with antibodies present in sera of infertile women has been studied using mAb H6-3C4, which produces immobilization of human sperm in the presence of complement. Another antibody, NUH2, which also induces human sperm immobilization, was used to substantiate the presence of a receptor on sperm involved in susceptibility to immobilization. Both antibodies defined type 2 chain polylactosamine structure. H6-3C4 is directed to internally located repetitive N-acetyllactosamine, i.e., sialyl-i, i, or fucosyl-i. NUH2 defines binary alpha 2----3 sialyl type 2 chain, i.e., sialyl-I. Thus, the presence of antibodies in the sera of infertile women directed to sperm lactosaminoglycan or lactosaminolipid could be the basis for infertility in these cases.

Sequential detection of mRNA for the chemokine IL-8 and macrophages (F4/80) in human melanoma

1995 ◽  
Vol 53 ◽  
pp. 1008-1009
Author(s):  
C.D. Bucana ◽  
R. Sanchez ◽  
R. Singh ◽  
I.J. Fidler
Keyword(s):  
Tumor Cells ◽  
Nude Mice ◽  
Constitutive Expression ◽  
Metastatic Potential ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Angiogenic Factor ◽  
Infiltrating Cells ◽  
Immunoperoxidase Assay ◽  
Multifunctional Cytokine

The purpose of this study was to demonstrate by ISH the presence of IL-8 mRNA, and by immunohistochemistry (IHC) the presence of the chemokine IL-8 and the distribution of infiltrating macrophages in subcutaneous melanomas in the same tumor. IL-8 is a multifunctional cytokine produced by melanoma cells, activated macrophages and monocytes and it has been shown to be a growth and angiogenic factor for tumor cells. More recently it was shown that constitutive expression of IL-8 correlated directly with metastatic potential of human melanoma cells in nude mice. IL-8 content of a solid tumor as determined by Western blot analysis does not take into account the contribution of macrophages. Previous studies showed that murine tumors contain many infiltrating cells interspersed among tumor cells whereas human tumors growing in nude mice exhibit macrophages at the periphery or between tumor islands. In this study we demonstrate the expression of IL-8 and the distribution of macrophages by immunoperoxidase assay and IL-8 mRNA by ISH.

1218: Organ Sparing Surgery in Small Testicular Tumors does not Lead to Development of Antisperm Antibodies

2007 ◽  
Vol 177 (4S) ◽  
pp. 401-401
Author(s):  
Nicolai Leonhartsberger ◽  
Thomas Akkad ◽  
Christian Gozzi ◽  
Georg Bartsch ◽  
Hannes Steiner
Keyword(s):  
Antisperm Antibodies ◽  
Testicular Tumors ◽  
Organ Sparing

The effect of altitude on absolute hearing threshold levels

1982 ◽  
Author(s):  
I. B. Mekjavic ◽  
D. Sinclair ◽  
D. Steele ◽  
N. S. Longridge
Keyword(s):  
Hearing Threshold ◽  
Threshold Levels

ECOLOGICAL AND TOXICOLOGICAL ASSESSMENT OF HAZARD, POSED BY WATER CONTAMINATION OF WATER BODIES WITH POLYVINYL NITRATE

2018 ◽  
pp. 12-19 ◽  
Author(s):  
A. A. Maslennikov ◽  
S. A. Demidova ◽  
A. V. Ryabova
Keyword(s):  
Biochemical Oxygen Demand ◽  
Water Contamination ◽  
Chronic Toxicity ◽  
Negative Impact ◽  
Oxygen Demand ◽  
Water Bodies ◽  
Organoleptic Properties ◽  
Toxicological Assessment ◽  
Threshold Levels ◽  
Polyvinyl Nitrate

Water containing polyvinyl nitrate was experimentally assessed on the basis of organoleptic, general sanitary and toxicological indicators of harmfulness. It was established that that the compound did not change water organoleptic properties but produced a negative impact on viability of saprophytic microflora , nitrification processes and biochemical oxygen demand. Besides, in tests on animals. the substance caused acute, sub-acute and chronic toxicity. Based on those signs of harmfulness, threshold levels of exposure were established. Data obtained were taken into account for substantiation of MAC (Maximum allowable concentration) of polyvinyl nitrate in water bodies.

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