Measurement of dermal water content by confocal RAMAN spectroscopy to investigate intrinsic aging and photoaging of human skin in vivo

2020 ◽  
Author(s):  
Ghaith Kourbaj ◽  
Stephan Bielfeldt ◽  
Matthias Seise ◽  
Klaus‐Peter Wilhelm
Keyword(s):  
Raman Spectroscopy ◽  
Water Content ◽  
Human Skin ◽  
Confocal Raman Spectroscopy ◽  
Confocal Raman

In Vivo Determination of Moisturizers Efficacy on Human Skin Hydration by Confocal Raman Spectroscopy

2018 ◽  
Vol 19 (7) ◽  
pp. 3177-3186 ◽  
Author(s):  
Vamshi Krishna Tippavajhala ◽  
Taciana D. Magrini ◽  
Daniele C. Matsuo ◽  
Michely G. P. Silva ◽  
Priscila P. Favero ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Human Skin ◽  
Skin Hydration ◽  
Confocal Raman Spectroscopy ◽  
Confocal Raman

scholarly journals Franz Cell Diffusion Testing and Quantitative Confocal Raman Spectroscopy: In Vitro-In Vivo Correlation

Pharmaceutics ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 887
Author(s):  
Fotis Iliopoulos ◽  
Peter J. Caspers ◽  
Gerwin J. Puppels ◽  
Majella E. Lane
Keyword(s):  
Raman Spectroscopy ◽  
Propylene Glycol ◽  
Human Skin ◽  
Ternary Mixtures ◽  
Drug Bioavailability ◽  
Confocal Raman Spectroscopy ◽  
Topical Formulations ◽  
Confocal Raman

Previously, we reported the use of Confocal Raman Spectroscopy (CRS) to investigate the topical delivery of actives and excipients. We have also correlated the results from CRS with findings from in vitro diffusion studies in human skin. However, until now CRS has only been used as a semi-quantitative method of determining the skin uptake of molecules, with results expressed as arbitrary units of signal intensity. Clearly, this posed challenges for using CRS to determine skin delivery and to assess the drug bioavailability and bioequivalence of topical formulations. In the present work, the permeation of niacinamide (NIA) from various formulations in human skin was studied in vitro using conventional Franz cells and in vivo using a quantitative CRS method under finite dose conditions. The selection of NIA was based on its wide use in pharmaceutical and personal care formulations for many years. This is the first fully quantitative study to compare these methods. The vehicles investigated were neat Transcutol® P (TC); binary combinations of propylene glycol (PG) with propylene glycol monolaurate (PGML); and ternary mixtures of PG, PGML, and isopropyl myristate (IPM). These solvents were selected to encompass a range of physicochemical properties. NIA permeation was evident from all formulations in vitro and in vivo. The vehicles PG:PGML and PG:PGML:IPM delivered comparable amounts across the skin in vitro at 24 h (100.3–106.7 µg/cm2, p > 0.05) that were significantly higher compared with those of TC (1.3 µg/cm2, p < 0.05). An excellent in vitro in vivo correlation (R2 = 0.98) was found following the linear regression of the cumulative amounts of NIA permeated in vitro and the amounts of NIA at 2 μm in the skin measured with CRS. A very good correlation between the cumulative permeation of NIA in vitro and the total amount of NIA that penetrated the stratum corneum (SC) per unit of surface area (μg/cm2) in vivo was also observed, with a Pearson correlation coefficient (R2) of 0.94. The findings support the use of CRS for the quantitative measurement of actives delivered to the skin in vivo. Future studies will focus on exploring the reproducibility and reliability of the method by investigating the delivery of different actives from a wider range of vehicles. Additionally, quantitative CRS will be evaluated further as a method for assessing the bioequivalence of topical formulations.

In vivo confocal Raman spectroscopy study of the vitamin A derivative perfusion through human skin

2016 ◽  
Author(s):  
Laurita dos Santos ◽  
Claudio A. Téllez Soto ◽  
Priscila P. Favero ◽  
Airton A. Martin
Keyword(s):  
Raman Spectroscopy ◽  
Vitamin A ◽  
Human Skin ◽  
Spectroscopy Study ◽  
Confocal Raman Spectroscopy ◽  
Confocal Raman ◽  
Vitamin A Derivative

In vivo determination of dermal water content in chronological skin aging by confocal Raman spectroscopy

2021 ◽  
Vol 112 ◽  
pp. 103196
Author(s):  
Claudio A. Téllez-Soto ◽  
Michely G. Pereira Silva ◽  
Laurita dos Santos ◽  
Thiago de O. Mendes ◽  
Priyanka Singh ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Water Content ◽  
Skin Aging ◽  
Confocal Raman Spectroscopy ◽  
Confocal Raman

scholarly journals Combined In Vivo Confocal Raman Spectroscopy and Confocal Microscopy of Human Skin

2003 ◽  
Vol 85 (1) ◽  
pp. 572-580 ◽  
Author(s):  
P.J. Caspers ◽  
G.W. Lucassen ◽  
G.J. Puppels
Keyword(s):  
Raman Spectroscopy ◽  
Confocal Microscopy ◽  
Human Skin ◽  
Confocal Raman Spectroscopy ◽  
Confocal Raman

scholarly journals In Vivo Human Skin Penetration Study of Sunscreens by Confocal Raman Spectroscopy

2017 ◽  
Vol 19 (2) ◽  
pp. 753-760 ◽  
Author(s):  
Vamshi Krishna Tippavajhala ◽  
Thiago de Oliveira Mendes ◽  
Airton Abrahão Martin
Keyword(s):  
Raman Spectroscopy ◽  
Human Skin ◽  
Skin Penetration ◽  
Confocal Raman Spectroscopy ◽  
Confocal Raman
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