Susceptibility of Pigs to Zoonotic Hepatitis E Virus Genotype 3 Isolated from a Wild Boar

2016 ◽  
Vol 64 (5) ◽  
pp. 1589-1597 ◽  
Author(s):  
D. Thiry ◽  
N. Rose ◽  
A. Mauroy ◽  
F. Paboeuf ◽  
L. Dams ◽  
...  
Keyword(s):  
Wild Boar ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype

DETECTION AND PHYLOGENETIC CHARACTERIZATION OF HEPATITIS E VIRUS GENOTYPE 3 IN A CAPTIVE WILD BOAR IN THAILAND

2012 ◽  
Vol 43 (3) ◽  
pp. 640-644 ◽  
Author(s):  
Anuwat Wiratsudakul ◽  
Ladawan Sariya ◽  
Phirom Prompiram ◽  
Siriporn Tantawet ◽  
Duangkhamol Suraruangchai ◽  
...  
Keyword(s):  
Wild Boar ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Phylogenetic Characterization

scholarly journals Hepatitis E Virus Genotype 3 Diversity: Phylogenetic Analysis and Presence of Subtype 3b in Wild Boar in Europe

Viruses ◽  
2015 ◽  
Vol 7 (5) ◽  
pp. 2704-2726 ◽  
Author(s):  
Ariel Vina-Rodriguez ◽  
Josephine Schlosser ◽  
Dietmar Becher ◽  
Volker Kaden ◽  
Martin Groschup ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Wild Boar ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype

scholarly journals Natural and experimental hepatitis E virus genotype 3 - infection in European wild boar is transmissible to domestic pigs

2014 ◽  
Vol 45 (1) ◽  
Author(s):  
Josephine Schlosser ◽  
Martin Eiden ◽  
Ariel Vina-Rodriguez ◽  
Christine Fast ◽  
Paul Dremsek ◽  
...  
Keyword(s):  
Wild Boar ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Domestic Pigs ◽  
Experimental Hepatitis ◽  
European Wild Boar

Emergent subtype of hepatitis E virus genotype 3 in wild boar in Spain

2019 ◽  
Vol 66 (5) ◽  
pp. 1803-1808 ◽  
Author(s):  
Javier Caballero‐Gómez ◽  
Saul Jiménez‐Ruiz ◽  
Pedro Lopez‐Lopez ◽  
Joaquin Vicente ◽  
Maria A. Risalde ◽  
...  
Keyword(s):  
Wild Boar ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype

scholarly journals Hepatitis E Virus Genotype 3 Strains in Domestic Pigs, Cameroon

2013 ◽  
Vol 19 (4) ◽  
pp. 686-688 ◽  
Author(s):  
Vanessa Salete de Paula ◽  
Matthias Wiele ◽  
Afegenwi H. Mbunkah ◽  
Achukwi M. Daniel ◽  
Manchang T. Kingsley ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Domestic Pigs

Hepatitis E virus genotype 3; an under diagnosed pathogen causing chronic hepatitis in solid organ transplant recipients

2014 ◽  
Vol 31 (4) ◽  
pp. 223
Author(s):  
A. Riezebos-Brilman ◽  
H. Blokzijl ◽  
J.S.F. Sanders ◽  
Verschuuren E.A.M. ◽  
H.G.M. Niesters
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis E Virus ◽  
Organ Transplant ◽  
Hepatitis E ◽  
Solid Organ Transplant ◽  
Solid Organ ◽  
Transplant Recipients ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Solid Organ Transplant Recipients

scholarly journals No Evidence for Hepatitis E Virus Genotype 3 Susceptibility in Rats

2013 ◽  
Vol 19 (8) ◽  
pp. 1343-1345 ◽  
Author(s):  
Tian-Cheng Li ◽  
Yasushi Ami ◽  
Yuriko Suzaki ◽  
Naokazu Takeda ◽  
Wakita Takaji
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 3 ◽  
Virus Genotype

scholarly journals Cell Culture Isolation and Whole Genome Characterization of Hepatitis E Virus Strains from Wild Boars in Germany

2021 ◽  
Vol 9 (11) ◽  
pp. 2302
Author(s):  
Katja Schilling-Loeffler ◽  
Oliver Viera-Segura ◽  
Victor Max Corman ◽  
Julia Schneider ◽  
Ashish K. Gadicherla ◽  
...  
Keyword(s):  
Cell Culture ◽  
Wild Boar ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Enriched Library ◽  
Genome Copy ◽  
Genotype 3 ◽  
Wild Boars ◽  
Copy Numbers ◽  
Cell Culture Isolation

Infection with hepatitis E virus (HEV) can cause acute and chronic hepatitis in humans. The HEV genotype 3 can be zoonotically transmitted from animals to humans, with wild boars representing an important reservoir species. Cell culture isolation of HEV is generally difficult and mainly described for human isolates so far. Here, five sera and five liver samples from HEV-RNA-positive wild boar samples were inoculated onto PLC/PRF/5 cells, incubated for 3 months and thereafter passaged for additional 6 weeks. As demonstrated by RT-qPCR, immunofluorescence and immune electron microscopy, virus was successfully isolated from two liver samples, which originally contained high HEV genome copy numbers. Both isolates showed slower growth than the culture-adapted HEV strain 47832c. In contrast to this strain, the isolated strains had no insertions in their hypervariable genome region. Next generation sequencing using an HEV sequence-enriched library enabled full genome sequencing. Strain Wb108/17 belongs to subtype 3f and strain Wb257/17 to a tentative novel subtype recently described in Italian wild boars. The results indicate that HEV can be successfully isolated in cell culture from wild boar samples containing high HEV genome copy numbers. The isolates may be used further to study the zoonotic potential of wild boar-derived HEV subtypes.

scholarly journals Comprehensive Molecular Approach for Characterization of Hepatitis E Virus Genotype 3 Variants

2018 ◽  
Vol 56 (5) ◽  
Author(s):  
Bo Wang ◽  
Dominik Harms ◽  
C. Patrick Papp ◽  
Sandra Niendorf ◽  
Sonja Jacobsen ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis E Virus ◽  
Phylogenetic Analyses ◽  
Hepatitis E ◽  
Full Length ◽  
Molecular Approach ◽  
Genotype 3 ◽  
Genome Sequences ◽  
Virus Genotype ◽  
Pcr Targeting

ABSTRACT Autochthonous hepatitis E virus genotype 3 (HEV-3) infections in industrialized countries are more frequent than previously assumed. HEV-3 is zoonotic and the causal pathogen of chronic hepatitis E. According to the latest classification of the family Hepeviridae , 10 designated HEV-3 subtypes (HEV-3a to HEV-3j) and 7 unassigned HEV-3 subtypes are proposed. In order to identify and characterize the HEV-3 variants in circulation, we developed a molecular approach combining a sensitive HEV-specific real-time reverse transcription-PCR (RT-PCR) targeting the overlapping region of HEV ORF2 and ORF3 (the ORF2/3 region) and two newly designed consensus nested RT-PCRs targeting the HEV ORF1 and ORF2 genes, respectively. Since complete genome sequences are required for new HEV-3 subtype assignment, we implemented a straightforward approach for full-length HEV-3 genome amplification. Twenty-nine human serum samples and six human feces samples from chronic hepatitis E patients were selected for evaluation of the system. Viral loads ranged from 1 × 10 4 to 1.9 × 10 10 copies/ml of serum and from 1.8 × 10 4 to 1 × 10 12 copies/g of feces. Sequence and phylogenetic analyses of partial ORF1 and ORF2 sequences showed that HEV strains had considerable genetic diversity and clustered into the HEV-3c (29/35), HEV-3e (2/35), HEV-3f (2/35), and unassigned HEV-3 (2/35) subtypes. Moreover, from these strains, three full-length HEV-3 genome sequences were generated and characterized. DE/15-0030 represents a typical HEV-3c strain (95.7% nucleotide identity to wbGER27), while DE/15-0031 and SW/16-0282 have <89.2% homology to known HEV-3 strains and are phylogenetically divergent, indicating novel HEV-3 subtypes. In summary, our approach will significantly facilitate the detection, quantification, and determination of HEV-3 strains and will thus help to improve molecular diagnostics and our knowledge of HEV diversity and evolution.

scholarly journals Hallmarks of liver lesions in pigs naturally infected by hepatitis E virus genotype 3

2018 ◽  
Vol 38 (1) ◽  
pp. 65-70
Author(s):  
Alex J.S. de Souza ◽  
Andreza P. Malheiros ◽  
Manoel do C.P. Soares ◽  
Michele S. Gomes-Gouvêa ◽  
João R.R. Pinho ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Molecular Techniques ◽  
Human Consumption ◽  
Liver Lesions ◽  
Genotype 3 ◽  
Virus Genotype ◽  
Liver Histopathology ◽  
Significant Difference ◽  
Group 2

ABSTRACT: Histopathological evaluation of liver from 33 pigs slaughtered for human consumption in Amazon region, previously tested by serology and molecular techniques for hepatitis E virus infection (HEV), was analysed in three groups: Group 1, negative for both HEV-RNA and anti-HEV IgG (n=10); Group 2, positive for HEV-RNA (n=13); Group 3, positive for anti-HEV IgG (n=10). Group 2 showed a significant difference among the groups for liver lesions such as lobular activity (P=0.007), periportal interface hepatitis (P=0.004), portal inflammation (P=0.028) hepatitis with lobular, portal and periportal interface activity (P=0.001). HEV detection by immunohistochemistry was performed and 3 of 6 samples of group 2 were positive. Pigs naturally infected by HEV genotype 3 present microscopic necroinflammatory liver lesions similar to HEV in humans. Liver histopathology showed be important in the diagnosis of active asymptomatic HEV infection in pigs slaughtered for human consumption because hepatic liver lesions may present distinct profiles according to molecular and serological diagnosis and in this sense, histopathology and immunohistochemistry may be an important complementary diagnostic tool.

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