Presence of Torque teno sus virus 1 and 2 in porcine circovirus 3-positive pigs

ABSTRACT Porcine circovirus 3 (PCV-3) DNA has been detected in serum samples from apparently healthy pigs as well as pigs with different clinical conditions. Molecular detection of PCV-3 was observed in swine serum samples from Southeastern - Brazil using a nested PCR designed specifically for this study. The epidemiology and clinical aspects of PCV-3 infection were evaluated. The samples originated from 154 pigs of both genders from different production phases and with different clinical presentations, sampled from 31 pig farms visited between 2013 and 2018. In this study, PCV-3 was detected in 26.7% of samples from all populations across varying ages. Statistical association (P=0.0285) was observed only between animals with respiratory signs and PCV-3; no PCV-3-positive animal had diarrhea. No statistical association was observed between PCV-3 and age, or gender of the pigs. Because PCV-3 is a newly discovered virus, there is very little information about its epidemiology. We hope that these data can help in future studies investigating PCV-3 epidemiology.

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Concurrent Infection of Torque Teno Sus Virus and Porcine Circovirus Type 2 in a Clinical Case with Post-weaning Multisystemic Wasting Syndrome

British Microbiology Research Journal ◽

10.9734/bmrj/2014/6336 ◽

2014 ◽

Vol 4 (6) ◽

pp. 607-615 ◽

Cited By ~ 1

Author(s):

Shao-Lun Zhai

Keyword(s):

Clinical Case ◽

Porcine Circovirus Type ◽

Porcine Circovirus Type 2 ◽

Porcine Circovirus ◽

Concurrent Infection ◽

Wasting Syndrome ◽

Torque Teno Sus Virus

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Detection and genetic characterization of porcine circovirus 3 from aborted fetuses and pigs with respiratory disease in Korea

Journal of Veterinary Science ◽

10.4142/jvs.2018.19.5.721 ◽

2018 ◽

Vol 19 (5) ◽

pp. 721 ◽

Cited By ~ 17

Author(s):

Seong-Hee Kim ◽

Ji-Young Park ◽

Ji-Youl Jung ◽

Ha-Young Kim ◽

Yu-Ri Park ◽

...

Keyword(s):

Respiratory Disease ◽

Genetic Characterization ◽

Porcine Circovirus ◽

Porcine Circovirus 3

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Development of a SYBR green I-based duplex real-time PCR assay for detection of pseudorabies virus and porcine circovirus 3

Molecular and Cellular Probes ◽

10.1016/j.mcp.2020.101593 ◽

2020 ◽

Vol 53 ◽

pp. 101593 ◽

Cited By ~ 1

Author(s):

Run-Bo Tian ◽

Yue Jin ◽

Tong Xu ◽

Yu Zhao ◽

Zhen-Ya Wang ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Pseudorabies Virus ◽

Sybr Green ◽

Sybr Green I ◽

Porcine Circovirus ◽

Pcr Assay ◽

Porcine Circovirus 3

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Sensitive detection of porcine circovirus 3 by droplet digital PCR

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638719847686 ◽

2019 ◽

Vol 31 (4) ◽

pp. 604-607

Author(s):

Yuqi Liu ◽

Hecheng Meng ◽

Lei Shi ◽

Lili Li

Keyword(s):

Digital Pcr ◽

Droplet Digital Pcr ◽

Porcine Circovirus ◽

Analytical Sensitivity ◽

Swine Industry ◽

Potential Threat ◽

Emerging Virus ◽

Clinical Detection ◽

Porcine Circovirus 3 ◽

High Degree

Porcine circovirus 3 (PCV-3) is a newly emerging virus that poses a potential threat to the swine industry. We developed a sensitive assay utilizing droplet digital PCR (ddPCR) to detect PCV-3. Specificity of the assay was confirmed by the failure of amplification of DNA of other relevant viruses. The detection limit for ddPCR was 1 copy/μL, 10 times greater sensitivity than TaqMan real-time PCR (rtPCR). Both methods showed a high degree of linearity, although TaqMan rtPCR showed less sensitivity than ddPCR for clinical detection. Our findings indicate that ddPCR might offer faster and improved analytical sensitivity for PCV-3 detection.

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