Enzymatic recombinase amplification coupled with CRISPR-Cas12a for ultrasensitive, rapid, and specific Porcine circovirus 3 detection

2021 ◽  
Vol 59 ◽  
pp. 101763
Author(s):  
Wuyin Zhang ◽  
Liang Xu ◽  
Qi Liu ◽  
Yingli Cao ◽  
Kankan Yang ◽  
...  
Keyword(s):  
Porcine Circovirus ◽  
Porcine Circovirus 3

Molecular Characterization of Novel Porcine Circovirus 3 (PCV3) in Pig Populations in the North of Vietnam

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Nguyen Van Giap ◽  
Chung Hee Chun ◽  
Huynh Thi My Le ◽  
Cao Thi Bich Phuong ◽  
Vu Thi Ngoc ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Porcine Circovirus ◽  
The North ◽  
Porcine Circovirus 3

Loop-mediated isothermal amplification assay for the rapid and visual detection of novel porcine circovirus 3

2018 ◽  
Vol 253 ◽  
pp. 26-30 ◽  
Author(s):  
Yu-Ri Park ◽  
Hye-Ryung Kim ◽  
Seong-Hee Kim ◽  
Kyoung-Ki Lee ◽  
Young S. Lyoo ◽  
...  
Keyword(s):  
Visual Detection ◽  
Isothermal Amplification ◽  
Porcine Circovirus ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay ◽  
Porcine Circovirus 3

scholarly journals Molecular detection and clinical aspects of porcine circovirus 3 infection in pigs from Brazil

2020 ◽  
Vol 72 (5) ◽  
pp. 1731-1736
Author(s):  
A.C.M. Cruz ◽  
I.L.F. Rodrigues ◽  
A.E. Souza ◽  
F.B. Knackfuss ◽  
R.L. Silveira ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Porcine Circovirus ◽  
Southeastern Brazil ◽  
Clinical Aspects ◽  
Statistical Association ◽  
Serum Samples ◽  
Clinical Presentations ◽  
Porcine Circovirus 3 ◽  
Clinical Conditions ◽  
Swine Serum

ABSTRACT Porcine circovirus 3 (PCV-3) DNA has been detected in serum samples from apparently healthy pigs as well as pigs with different clinical conditions. Molecular detection of PCV-3 was observed in swine serum samples from Southeastern - Brazil using a nested PCR designed specifically for this study. The epidemiology and clinical aspects of PCV-3 infection were evaluated. The samples originated from 154 pigs of both genders from different production phases and with different clinical presentations, sampled from 31 pig farms visited between 2013 and 2018. In this study, PCV-3 was detected in 26.7% of samples from all populations across varying ages. Statistical association (P=0.0285) was observed only between animals with respiratory signs and PCV-3; no PCV-3-positive animal had diarrhea. No statistical association was observed between PCV-3 and age, or gender of the pigs. Because PCV-3 is a newly discovered virus, there is very little information about its epidemiology. We hope that these data can help in future studies investigating PCV-3 epidemiology.

scholarly journals Detection and genetic characterization of porcine circovirus 3 from aborted fetuses and pigs with respiratory disease in Korea

2018 ◽  
Vol 19 (5) ◽  
pp. 721 ◽  
Author(s):  
Seong-Hee Kim ◽  
Ji-Young Park ◽  
Ji-Youl Jung ◽  
Ha-Young Kim ◽  
Yu-Ri Park ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Genetic Characterization ◽  
Porcine Circovirus ◽  
Porcine Circovirus 3

scholarly journals Development of a SYBR green I-based duplex real-time PCR assay for detection of pseudorabies virus and porcine circovirus 3

2020 ◽  
Vol 53 ◽  
pp. 101593 ◽  
Author(s):  
Run-Bo Tian ◽  
Yue Jin ◽  
Tong Xu ◽  
Yu Zhao ◽  
Zhen-Ya Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pseudorabies Virus ◽  
Sybr Green ◽  
Sybr Green I ◽  
Porcine Circovirus ◽  
Pcr Assay ◽  
Porcine Circovirus 3

scholarly journals Sensitive detection of porcine circovirus 3 by droplet digital PCR

2019 ◽  
Vol 31 (4) ◽  
pp. 604-607
Author(s):  
Yuqi Liu ◽  
Hecheng Meng ◽  
Lei Shi ◽  
Lili Li
Keyword(s):  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Porcine Circovirus ◽  
Analytical Sensitivity ◽  
Swine Industry ◽  
Potential Threat ◽  
Emerging Virus ◽  
Clinical Detection ◽  
Porcine Circovirus 3 ◽  
High Degree

Porcine circovirus 3 (PCV-3) is a newly emerging virus that poses a potential threat to the swine industry. We developed a sensitive assay utilizing droplet digital PCR (ddPCR) to detect PCV-3. Specificity of the assay was confirmed by the failure of amplification of DNA of other relevant viruses. The detection limit for ddPCR was 1 copy/μL, 10 times greater sensitivity than TaqMan real-time PCR (rtPCR). Both methods showed a high degree of linearity, although TaqMan rtPCR showed less sensitivity than ddPCR for clinical detection. Our findings indicate that ddPCR might offer faster and improved analytical sensitivity for PCV-3 detection.

scholarly journals Frequency of Detection and Phylogenetic Analysis of Porcine circovirus 3 (PCV-3) in Healthy Primiparous and Multiparous Sows and Their Mummified Fetuses and Stillborn

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 533 ◽  
Author(s):  
Viviane Saporiti ◽  
Susanna Martorell ◽  
Taís F. Cruz ◽  
Francini Klaumann ◽  
Florencia Correa-Fiz ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Porcine Circovirus ◽  
Reproductive Parameters ◽  
Reproductive Disorders ◽  
Time Points ◽  
Lack Of Information ◽  
Complete Genomes ◽  
Reproductive Disease ◽  
Porcine Circovirus 3 ◽  
Intrauterine Infections

Porcine circovirus 3 (PCV-3) has been suggested as a putative causal agent of swine reproductive disease. A number of different studies have pointed out this association, but there is still a lack of information regarding the normal rates of PCV-3 infection in farms with normal reproductive parameters. The objective of the present study was to assess the frequency of PCV-3 detection in primiparous and multiparous sows and in tissues from their respective fetuses from farms with average reproductive parameters. Sera from 57 primiparous and 64 multiparous sows from 3 different farms were collected at two time points. Brain and lung tissues from 49 mummies and 206 stillborn were collected at farrowing. Samples were tested by PCR, and when positive, quantified by quantitative PCR. Thirty-nine complete genomes were obtained and phylogenetically analyzed. All sera from multiparous sows were negative, while 19/57 (33.3%) primiparous sows were PCV-3 PCR positive. From the 255 tested fetuses, 86 (33.7%) had at least one tissue positive to PCV-3. The frequency of detection in fetuses from primiparous sows (73/91, 80.2%) was significantly higher than those from multiparous ones (13/164, 7.9%). It can be concluded that PCV-3 is able to cause intrauterine infections in absence of overt reproductive disorders.

scholarly journals An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China

2019 ◽  
Vol 270 ◽  
pp. 197663 ◽  
Author(s):  
Wenchao Sun ◽  
Wei Wang ◽  
Jialiang Xin ◽  
Liang Cao ◽  
Xinyu Zhuang ◽  
...  
Keyword(s):  
Porcine Circovirus ◽  
Epidemiological Investigation ◽  
Guangxi Province ◽  
Porcine Circovirus 3

Prevalence of novel porcine circovirus 3 in Korean pig populations

2017 ◽  
Vol 207 ◽  
pp. 178-180 ◽  
Author(s):  
Taeyong Kwon ◽  
Sung J. Yoo ◽  
Choi-Kyu Park ◽  
Young S. Lyoo
Keyword(s):  
Porcine Circovirus ◽  
Porcine Circovirus 3

scholarly journals Genotyping Porcine Circovirus 3 (PCV-3) Nowadays: Does It Make Sense?

Viruses ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 265 ◽  
Author(s):  
Giovanni Franzo ◽  
Eric Delwart ◽  
Robert Fux ◽  
Ben Hause ◽  
Shuo Su ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Complete Genome ◽  
Molecular Data ◽  
Porcine Circovirus ◽  
Bootstrap Support ◽  
Research Activity ◽  
Reading Frame ◽  
Porcine Circovirus 3 ◽  
Definition Of ◽  
Genomic Regions

The discovery of a globally distributed porcine circovirus (Porcine circovirus 3; PCV-3) has led to intense research activity and the production of a large amount of molecular data. Different research groups have proposed several, not always concordant, genotypes for this virus. While such categories could aid an easier interpretation of PCV-3 molecular epidemiology, any classification, to be useful in practical settings, must be univocal and of help in the understanding of underlying biological features and epidemiology. Based on these premises, the possibility of defining PCV-3 genotypes was evaluated on the broadest available dataset of PCV-3 complete genome (n = 357) and open reading frame 2 (ORF2, n = 653) sequences. Genetic distance and phylogenetic clustering were selected as the main objective criteria. Additional factors, including the number of within-cluster sequences, host and geographic clustering, concordance between different genomic regions, and analysis method were also taken in account to generate a classification that could be effectively applied in research and diagnostic settings. A maximum within-genotype genetic distance of 3% at the complete genome and 6% at the ORF2 levels, bootstrap support higher than 90%, and concordance between analysis methods allowed us to clearly define two clades which could be potentially defined as genotypes. Further subdivision was not suggested due to the absence of a meaningful association between PCV-3 and its biological/epidemiological features. Nevertheless, since one of the clades included two strains only, thus far we formally propose the definition of only one PCV-3 genotype (PCV-3a). The established criteria will allow us to automatically recognize other genotypes when more strain sequences are characterized.

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