Specific detection and differentiation of tick‐borne encephalitis and West Nile virus induced IgG antibodies in humans and horses

Author(s):  
Alexandra Rockstroh ◽  
Beyene Moges ◽  
Beatrice S. Berneck ◽  
Tatjana Sattler ◽  
Sandra Revilla‐Fernández ◽  
...  
Biologia ◽  
2019 ◽  
Vol 74 (7) ◽  
pp. 813-820 ◽  
Author(s):  
Tomáš Csank ◽  
Ľuboš Korytár ◽  
Terézia Pošiváková ◽  
Tamás Bakonyi ◽  
Juraj Pistl ◽  
...  

2017 ◽  
Vol 89 (10) ◽  
pp. 1875-1878 ◽  
Author(s):  
Iva Christova ◽  
Elitsa Panayotova ◽  
Simona Tchakarova ◽  
Evgenia Taseva ◽  
Iva Trifonova ◽  
...  

2011 ◽  
Vol 16 (10) ◽  
Author(s):  
P Pezzotti ◽  
C Piovesan ◽  
L Barzon ◽  
R Cusinato ◽  
M Cattai ◽  
...  

Binary file ES_Abstracts_Final_ECDC.txt matches


Viruses ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 720
Author(s):  
Nataša Knap ◽  
Miša Korva ◽  
Vladimir Ivović ◽  
Katja Kalan ◽  
Mateja Jelovšek ◽  
...  

West Nile virus (WNV) is a flavivirus transmitted by mosquitoes. Birds are the reservoir for the virus; humans, horses and other mammals are dead-end hosts. Infections caused by WNV in humans can vary from asymptomatic infections to West Nile fever (WNF) or West Nile neuroinvasive disease (WNND). In 1995, a serosurvey was performed in Slovenia on forest workers, and WNV specific IgG antibodies were confirmed in 6.8% of the screened samples, indicating that WNV is circulating in Slovenia. No human disease cases were detected in Slovenia until 2013, when the first case of WNV infection was confirmed in a retrospective study in a 79-year old man with meningitis. In 2018, three patients with WNND were confirmed by laboratory tests, with detection of IgM antibodies in the cerebrospinal fluid of the patients. In one of the patients, WNV RNA was detected in the urine sample. In 2017, 2018 and 2019, a mosquito study was performed in Slovenia. Mosquitoes were sampled on 14 control locations and 35 additional locations in 2019. No WNV was detected in mosquitoes in 2017 and 2019, but we confirmed the virus in a pool of Culex sp. mosquitoes in 2018. The virus was successfully isolated, and complete genome sequence was acquired. The whole genome of the WNV was also sequenced from the patient’s urine sample. The whole genome sequences of the WNV virus detected in Slovenian patient and mosquito indicate the virus most likely spread from the north, because of the geographic proximity and because the sequences cluster with the Austrian and Hungarian sequences. A sentinel study was performed on dog sera samples, and we were able to confirm IgG antibodies in 1.8% and 4.3% of the samples in 2017 and 2018, respectively. Though Slovenia is not a highly endemic country for WNV, we have established that the virus circulates in Slovenia.


2019 ◽  
Vol 19 (6) ◽  
pp. 446-449 ◽  
Author(s):  
Huseyin Yilmaz ◽  
Kenan Barut ◽  
Asiye Karakullukcu ◽  
Ozgur Kasapcopur ◽  
Bekir Kocazeybek ◽  
...  

2008 ◽  
Vol 40 (9) ◽  
pp. 759-761 ◽  
Author(s):  
Emöke Ferenczi ◽  
Enikö Bán ◽  
Anita Ábrahám ◽  
Tamásné Kaposi ◽  
Gábor Petrányi ◽  
...  

2011 ◽  
Vol 156 (9) ◽  
pp. 1661-1663 ◽  
Author(s):  
Anna Papa ◽  
Kostas Danis ◽  
Katerina Tsergouli ◽  
Katerina Tsioka ◽  
Elpida Gavana

2016 ◽  
Vol 161 (6) ◽  
pp. 1679-1683 ◽  
Author(s):  
Tomáš Csank ◽  
Katarína Bhide ◽  
Elena Bencúrová ◽  
Saskia Dolinská ◽  
Petra Drzewnioková ◽  
...  

2014 ◽  
Vol 143 (9) ◽  
pp. 1931-1935 ◽  
Author(s):  
A. ZOHAIB ◽  
M. SAQIB ◽  
C. BECK ◽  
M. H. HUSSAIN ◽  
S. LOWENSKI ◽  
...  

SUMMARYThis study describes the first large-scale serosurvey on West Nile virus (WNV) conducted in the equine population in Pakistan. Sera were collected from 449 equids from two provinces of Pakistan during 2012–2013. Equine serum samples were screened using a commercial ELISA kit detecting antibodies against WNV and related flaviviruses. ELISA-positive samples were further investigated using virus-specific microneutralization tests (MNTs) to identify infections with Japanese encephalitis virus (JEV), WNV and tick-borne encephalitis virus (TBEV). Anti-WNV antibodies were detected in 292 samples by ELISA (seroprevalence 65·0%) and WNV infections were confirmed in 249 animals by MNT. However, there was no animal found infected by JEV or TBEV. The detection of WNV-seropositive equines in Pakistan strongly suggests a widespread circulation of WNV in Pakistan.


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