Surveillance on antibodies against West Nile virus, Usutu virus, tick-borne encephalitis virus and Tribeč virus in wild birds in Drienovská wetland, Slovakia

Biologia ◽  
2019 ◽  
Vol 74 (7) ◽  
pp. 813-820 ◽  
Author(s):  
Tomáš Csank ◽  
Ľuboš Korytár ◽  
Terézia Pošiváková ◽  
Tamás Bakonyi ◽  
Juraj Pistl ◽  
...  
2017 ◽  
Vol 89 (10) ◽  
pp. 1875-1878 ◽  
Author(s):  
Iva Christova ◽  
Elitsa Panayotova ◽  
Simona Tchakarova ◽  
Evgenia Taseva ◽  
Iva Trifonova ◽  
...  

2016 ◽  
Vol 161 (6) ◽  
pp. 1679-1683 ◽  
Author(s):  
Tomáš Csank ◽  
Katarína Bhide ◽  
Elena Bencúrová ◽  
Saskia Dolinská ◽  
Petra Drzewnioková ◽  
...  

2014 ◽  
Vol 143 (9) ◽  
pp. 1931-1935 ◽  
Author(s):  
A. ZOHAIB ◽  
M. SAQIB ◽  
C. BECK ◽  
M. H. HUSSAIN ◽  
S. LOWENSKI ◽  
...  

SUMMARYThis study describes the first large-scale serosurvey on West Nile virus (WNV) conducted in the equine population in Pakistan. Sera were collected from 449 equids from two provinces of Pakistan during 2012–2013. Equine serum samples were screened using a commercial ELISA kit detecting antibodies against WNV and related flaviviruses. ELISA-positive samples were further investigated using virus-specific microneutralization tests (MNTs) to identify infections with Japanese encephalitis virus (JEV), WNV and tick-borne encephalitis virus (TBEV). Anti-WNV antibodies were detected in 292 samples by ELISA (seroprevalence 65·0%) and WNV infections were confirmed in 249 animals by MNT. However, there was no animal found infected by JEV or TBEV. The detection of WNV-seropositive equines in Pakistan strongly suggests a widespread circulation of WNV in Pakistan.


Viruses ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1873
Author(s):  
Phebe de Heus ◽  
Jolanta Kolodziejek ◽  
Zdenĕk Hubálek ◽  
Katharina Dimmel ◽  
Victoria Racher ◽  
...  

The emergence of West Nile virus (WNV) and Usutu virus (USUV) in addition to the autochthonous tick-borne encephalitis virus (TBEV) in Europe causes rising concern for public and animal health. The first equine case of West Nile neuroinvasive disease in Austria was diagnosed in 2016. As a consequence, a cross-sectional seroprevalence study was conducted in 2017, including 348 equids from eastern Austria. Serum samples reactive by ELISA for either flavivirus immunoglobulin G or M were further analyzed with the plaque reduction neutralization test (PRNT-80) to identify the specific etiologic agent. Neutralizing antibody prevalences excluding vaccinated equids were found to be 5.3% for WNV, 15.5% for TBEV, 0% for USUV, and 1.2% for WNV from autochthonous origin. Additionally, reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed to detect WNV nucleic acid in horse sera and was found to be negative in all cases. Risk factor analysis did not identify any factors significantly associated with seropositivity.


2018 ◽  
Vol 65 (3) ◽  
pp. 765-772 ◽  
Author(s):  
I. García-Bocanegra ◽  
E. Jurado-Tarifa ◽  
D. Cano-Terriza ◽  
R. Martínez ◽  
J. E. Pérez-Marín ◽  
...  

2019 ◽  
Vol 11 (2) ◽  
pp. 53-70 ◽  
Author(s):  
Tamaš Petrović ◽  
Milanko Šekler ◽  
Dušan Petrić ◽  
Dejan Vidanović ◽  
Aleksandar Potkonjak ◽  
...  

The aim of this study is to summarize the data on the occurrence, presence and prevalence of some zoonotic flaviviruses, which have been actively circulating in the territory of the Republic of Serbia during past decade. The genus Flavivirus, family Flaviviridae,encompasses vector-transmitted viruses with zoonotic potential. The genus is comprised of more than 70 viruses transmitted to humans by mosquitoes and ticks. Some of those are associated only with human diseases (such as yellow fever and dengue fever), whereas others can cause diseases in both humans and animals. Animals are major reservoirs and primary hosts for the latter group. The virus transmission to other animal species or humans occurs via diverse vectors – mostly mosquitoes and ticks. A range of these virus species is widely distributed worldwide, especially in tropical and sub-tropical climatic zones. Some representatives of these species have only recently been identified at the territory of Republic of Serbia. This paper gives an evidence on the presence and distribution of West-Nile virus, tick-borne encephalitis (TBE) virus and Usutu virus that were conducted within the study in Serbia. The research pointed out the presence of recently detected West-Nile virus and Usutu virus as well as the current circulation of tick-borne encephalitis virus, for which only limited serological evidence on the presence of virus-specific antibodies in humans was available so far. In the aspect of public health, the full understanding of the importance of identification and prevalence of different flaviviruses still require further comprehensive entomologicalacarological, seroepidemiological, clinical and virological research. 


Viruses ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2404
Author(s):  
Sebastian Napp ◽  
Francisco Llorente ◽  
Cécile Beck ◽  
Eduard Jose-Cunilleras ◽  
Mercè Soler ◽  
...  

The surveillance for West Nile virus (WNV) in Catalonia (northeastern Spain) has consistently detected flaviviruses not identified as WNV. With the aim of characterizing the flaviviruses circulating in Catalonia, serum samples from birds and horses collected between 2010 and 2019 and positive by panflavivirus competition ELISA (cELISA) were analyzed by microneutralization test (MNT) against different flaviviruses. A third of the samples tested were inconclusive by MNT, highlighting the limitations of current diagnostic techniques. Our results evidenced the widespread circulation of flaviviruses, in particular WNV, but also Usutu virus (USUV), and suggest that chicken and horses could serve as sentinels for both viruses. In several regions, WNV and USUV overlapped, but no significant geographical aggregation was observed. Bagaza virus (BAGV) was not detected in birds, while positivity to tick-borne encephalitis virus (TBEV) was sporadically detected in horses although no endemic foci were observed. So far, no human infections by WNV, USUV, or TBEV have been reported in Catalonia. However, these zoonotic flaviviruses need to be kept under surveillance, ideally within a One Health framework.


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