A chemiluminescence immunoassay for rapid detection of classical swine fever virus E2 antibodies in pig serum samples

2020 ◽  
Author(s):  
Zhongyuan Ma ◽  
Jianliang Lv ◽  
Zhongwang Zhang ◽  
Ye Zhao ◽  
Li Pan ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Rapid Detection ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Serum Samples ◽  
Chemiluminescence Immunoassay ◽  
Pig Serum

Rapid Preparation of Pure Antibodies against Classical Swine Fever Virus from Pig Serum by Immunoaffinity Chromatography

2011 ◽  
Vol 201-203 ◽  
pp. 691-695
Author(s):  
Gui Zhen Wang ◽  
Lin Feng ◽  
Yang Li ◽  
Ren Qiang Li
Keyword(s):  
Classical Swine Fever Virus ◽  
Extraction Efficiency ◽  
Classical Swine Fever ◽  
Normal Activity ◽  
Fever Virus ◽  
Total Proteins ◽  
Rapid Preparation ◽  
Sds Page ◽  
Sepharose 4B ◽  
Pig Serum

After Sepharose-4B polymerbeads were activated by using epichlorohydrin, purified swine fever virus as a ligand were binded with them to prepare an immobilized affinity chromatography column, which was used to prepare antibody from high immune pig serum. Equilibrated with pH 7.4, 0.02 mol/L PBS and eluated with pH 3.4, 0.2 mol/L NaAc–HAc buffer containing 0.5 mol/L NaCl, the purified protein obtained from this columne was demonstrated to have normal activity to combine with classical swine fever virus by SDS-PAGE and ELISA. The extraction efficiency of the antibody was 2.45% of total proteins. This study offers a novel, rapid and effective method for preparation of pure antibodies against classical swine fever virus from high immune pig serum.

Development and evaluation of rapid detection of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

2010 ◽  
Vol 146 (4) ◽  
pp. 147-150 ◽  
Author(s):  
Shuanghui Yin ◽  
Youjun Shang ◽  
Guangqing Zhou ◽  
Hong Tian ◽  
Yanhong Liu ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Classical Swine Fever Virus ◽  
Rapid Detection ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

scholarly journals Serum neutralization as a differential serological test for classical swine fever virus and other pestivirus infections

1999 ◽  
Vol 51 (5) ◽  
pp. 403-408 ◽  
Author(s):  
J.C.M. Paredes ◽  
E.A.S. Oliveira ◽  
L.G. Oliveira ◽  
J.C.A. Rosa ◽  
P.M. Roehe
Keyword(s):  
Classical Swine Fever Virus ◽  
Serological Test ◽  
Sampling Strategy ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Border Disease Virus ◽  
Serum Samples ◽  
Diarrhea Virus ◽  
Serum Neutralization ◽  
Border Disease

Serum neutralization tests (SN) were performed against classical swine fever virus (CSFV), bovine viral diarrhea virus (BVDV) and border disease virus (BDV) on samples of swine serum collected for screening of antibodies to CSFV, in order to determine the SN value as a differential serological test. Ninety-nine sera out of a sample of 16,664 were positive for antibodies to pestiviruses in an ELISA test which did not distinguish antibodies to different pestiviruses. When submitted to SN, 81 sera were positive for CSFV antibodies only. In 17 sera, crossreactive antibodies to either CSFV, BVDV or BDV were detected. In most of these sera (13 out of 17) the differences between SN titres against the three viruses were not sufficient to estimate which was the most likely antibody-inducing virus. It was concluded that, for the SN to be useful in such differentiation, it is essential to examine a sample which must include a representative number of sera from the same farm where suspect animals were detected. When isolated serum samples are examined, such as those obtained with the sampling strategy adopted here, the SN may give rise to inconclusive results.

scholarly journals Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya

VirusDisease ◽  
2018 ◽  
Vol 29 (1) ◽  
pp. 89-95 ◽  
Author(s):  
A. K. Chakraborty ◽  
A. Karam ◽  
P. Mukherjee ◽  
L. Barkalita ◽  
P. Borah ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Serum Samples ◽  
E2 Gene ◽  
Cattle Serum ◽  
Cattle Herds

scholarly journals Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever

1999 ◽  
Vol 19 (3-4) ◽  
pp. 123-127 ◽  
Author(s):  
Julio Cesar Muñoz Paredes ◽  
Liliane Guimarães Oliveira ◽  
Alexandre de Carvalho Braga ◽  
Iara Maria Trevisol ◽  
Paulo Michel Roehe
Keyword(s):  
Classical Swine Fever Virus ◽  
Predictive Value ◽  
Neutralizing Antibodies ◽  
Large Scale ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Serological Diagnosis ◽  
Immunoperoxidase Staining ◽  
Serum Samples ◽  
Swine Serum

An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivity; 92.86% specificity, 81.11% positive predictive value, 99.4% negative predictive value and a 94.1% precision. Statistical analysis showed a very strong correlation (r=0,94) between both tests. When compared to a commercially available ELISA kit, the performance of both, in relation to the NPLA, was similar. It was concluded that the ELISA-I is suitable for large scale screening of antibodies to classical swine fever virus, although it does not distinguish antibodies to classical swine fever virus from those induced by other pestiviruses.

Development of an immunochromatographic strip for rapid detection of antibodies against classical swine fever virus

2012 ◽  
Vol 180 (1-2) ◽  
pp. 32-37 ◽  
Author(s):  
Xuewu Li ◽  
Li Wang ◽  
Xibao Shi ◽  
Dong Zhao ◽  
Jifei Yang ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Rapid Detection ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Immunochromatographic Strip

Development of functionalized gold nanoparticles as nanoflare probes for rapid detection of classical swine fever virus

2018 ◽  
Vol 171 ◽  
pp. 110-114 ◽  
Author(s):  
Pengbo Ning ◽  
Zhongxing Wu ◽  
Xuepeng Li ◽  
Yulu Zhou ◽  
Aoxue Hu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Classical Swine Fever Virus ◽  
Rapid Detection ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Functionalized Gold Nanoparticles

scholarly journals Rapid Detection of Classical Swine Fever Virus by a Portable Real-Time Reverse Transcriptase PCR Assay

2003 ◽  
Vol 41 (1) ◽  
pp. 500-505 ◽  
Author(s):  
G. R. Risatti ◽  
J. D. Callahan ◽  
W. M. Nelson ◽  
M. V. Borca
Keyword(s):  
Reverse Transcriptase ◽  
Real Time ◽  
Classical Swine Fever Virus ◽  
Rapid Detection ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Pcr Assay ◽  
Reverse Transcriptase Pcr
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