Targeted gene disruption of ATP synthases 6‐1 and 6‐2 in the mitochondrial genome of Arabidopsis thaliana by mitoTALENs

2020 ◽  
Vol 104 (6) ◽  
pp. 1459-1471
Author(s):  
Shin‐ichi Arimura ◽  
Hiroki Ayabe ◽  
Hajime Sugaya ◽  
Miki Okuno ◽  
Yoshiko Tamura ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Mitochondrial Genome ◽  
Gene Disruption ◽  
Targeted Gene Disruption ◽  
Atp Synthases

scholarly journals copia-, gypsy- and LINE-Like Retrotransposon Fragments in the Mitochondrial Genome of Arabidopsis thaliana

Genetics ◽  
1996 ◽  
Vol 142 (2) ◽  
pp. 579-585 ◽  
Author(s):  
Volker Knoop ◽  
Michael Unseld ◽  
Joachim Marienfeld ◽  
Petra Brandt ◽  
Sabine Sünkel ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Mitochondrial Genome ◽  
Evolutionary History ◽  
Nuclear Genome ◽  
Plant Mitochondrial Genome ◽  
Gypsy Group ◽  
History Of

Abstract Several retrotransposon fragments are integrated in the mitochondrial genome of Arabidopsis thaliana. These insertions are derived from all three classes of nuclear retrotransposons, the Tyl/copia, Ty3/gypsy- and non-LTR/LINE-families. Members of the Ty3/gypsy group of elements have not yet been identified in the nuclear genome of Arabidopsis. The varying degrees of similarity with nuclear elements and the dispersed locations of the sequences in the mitochondrial genome suggest numerous independent transfer-insertion events in the evolutionary history of this plant mitochondrial genome. Overall, we estimate remnants of retrotransposons to cover ≥5% of the mitochondrial genome in Arabidopsis.

scholarly journals Development of a targeted gene disruption system in the PET-degrading bacterium Ideonella sakaiensis and its applications to PETase and MHETase genes

2021 ◽  
Author(s):  
Shin-ichi Hachisuka ◽  
Tarou Nishii ◽  
Shosuke Yoshida
Keyword(s):  
Terephthalic Acid ◽  
Parent Strain ◽  
Gene Disruption ◽  
Ethylene Terephthalate ◽  
Targeted Gene Disruption ◽  
Degrading Bacterium ◽  
Poly Ethylene Terephthalate ◽  
Poly Ethylene ◽  
Pet Hydrolase

Poly(ethylene terephthalate) (PET) is a commonly used synthetic plastic; however its non-biodegradability results in a large amount of waste accumulation that has a negative impact on the environment. Recently, a PET-degrading bacterium Ideonella sakaiensis 201-F6 strain was isolated and the enzymes involved in PET-digestion, PET hydrolase (PETase) and mono(2-hydroxyethyl) terephthalic acid (MHET) hydrolase (MHETase), were identified. Despite the great potentials of I. sakaiensis in bioremediation and biorecycling, approaches to studying this bacterium remain limited. In this study, to enable the functional analysis of PETase and MHETase genes in vivo , we have developed a gene disruption system in I. sakaiensis . The pT18 mobsacB -based disruption vector harboring directly connected 5'- and 3'-flanking regions of the target gene for homologous recombination was introduced into I. sakaiensis cells via conjugation. First, we deleted the orotidine 5'-phosphate decarboxylase gene ( pyrF ) from the genome of the wild-type strain, producing the Δ pyrF strain with 5-fluoroorotic acid (5-FOA) resistance. Next, using the Δ pyrF strain as a parent strain, and pyrF as a counterselection marker, we disrupted the genes for PETase and MHETase. The growth of both Δ petase and Δ mhetase strains on terephthalic acid (TPA, one of the PET hydrolytic products) was comparable to that of the parent strain. However, these mutant strains dramatically decreased the growth level on PET to that on no carbon source. Moreover, the Δ petase strain completely abolished PET degradation capacity. These results demonstrate that PETase and MHETase are essential for I. sakaiensis metabolism of PET. IMPORTANCE The poly(ethylene terephthalate) (PET)-degrading bacterium Ideonella sakaiensis possesses two unique enzymes able to serve in PET hydrolysis. PET hydrolase (PETase) hydrolyzes PET into mono(2-hydroxyethyl) terephthalic acid (MHET) and MHET hydrolase (MHETase) hydrolyzes MHET into terephthalic acid (TPA) and ethylene glycol (EG). These enzymes have attracted global attention as they have potential to be used for bioconversion of PET. Compared to many in vitro studies including the biochemical and crystal structure analyses, few in vivo studies have been reported. Here, we developed a targeted gene disruption system in I. sakaiensis , which was then applied for constructing Δ petase and Δ mhetase strains. Growth of these disruptants revealed that PETase is a sole enzyme responsible for PET degradation in I. sakaiensis , while PETase and MHETase play essential roles in its PET assimilation.

scholarly journals Targeted Gene Disruption in Zebrafish Reveals Noncanonical Functions of LH Signaling in Reproduction

2014 ◽  
Vol 28 (11) ◽  
pp. 1785-1795 ◽  
Author(s):  
Lianhe Chu ◽  
Jianzhen Li ◽  
Yun Liu ◽  
Wei Hu ◽  
Christopher H. K. Cheng
Keyword(s):  
Gene Disruption ◽  
Targeted Gene Disruption

scholarly journals Targeted Gene Disruption in theXenopus tropicalisGenome using Designed TALE Nucleases

2013 ◽  
Vol 30 (6) ◽  
pp. 455-460 ◽  
Author(s):  
Keisuke Nakajima ◽  
Yuya Nakai ◽  
Morihiro Okada ◽  
Yoshio Yaoita
Keyword(s):  
Gene Disruption ◽  
Targeted Gene Disruption

scholarly journals High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos

Biology Open ◽  
2013 ◽  
Vol 2 (5) ◽  
pp. 448-452 ◽  
Author(s):  
K.-i. T. Suzuki ◽  
Y. Isoyama ◽  
K. Kashiwagi ◽  
T. Sakuma ◽  
H. Ochiai ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Xenopus Laevis ◽  
Gene Disruption ◽  
High Efficiency ◽  
Targeted Gene Disruption ◽  
Xenopus Laevis Embryos

Immunocytochemical Analyses and Targeted Gene Disruption of GTPBP1

2000 ◽  
Vol 20 (17) ◽  
pp. 6195-6200
Author(s):  
Satoru Senju ◽  
Ken-ichi Iyama ◽  
Hironori Kudo ◽  
Shinichi Aizawa ◽  
Yasuharu Nishimura
Keyword(s):  
Gene Disruption ◽  
Targeted Gene Disruption

scholarly journals p53 Hinders CRISPR/Cas9-Mediated Targeted Gene Disruption in Memory CD8 T Cells In Vivo

2020 ◽  
Vol 205 (8) ◽  
pp. 2222-2230
Author(s):  
Samarchith P. Kurup ◽  
Steven J. Moioffer ◽  
Lecia L. Pewe ◽  
John T. Harty
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Gene Disruption ◽  
Memory Cd8 T Cells ◽  
Targeted Gene Disruption
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