Frequency of allotype “b” in human platelet antigen 1 to 29 systems among blood donors in Japan estimated using high‐resolution melt analysis

Transfusion ◽  
2020 ◽  
Vol 60 (11) ◽  
pp. 2702-2713
Author(s):  
Tomoya Hayashi ◽  
Ryota Aminaka ◽  
Hiroyuki Ishii ◽  
Yoshihiko Tani ◽  
Yoshihiro Fujimura ◽  
...  
Keyword(s):  
High Resolution ◽  
Blood Donors ◽  
Human Platelet ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Platelet Antigen ◽  
Human Platelet Antigen

Human platelet antigen genotypes in Turkish and Caucasian blood donors in Germany

2012 ◽  
Vol 80 (3) ◽  
pp. 214-218 ◽  
Author(s):  
B. Hauck-Dlimi ◽  
K. Hammon ◽  
R. Eckstein ◽  
S. Ott ◽  
R. Zimmermann ◽  
...  
Keyword(s):  
Blood Donors ◽  
Human Platelet ◽  
Platelet Antigen ◽  
Human Platelet Antigen

scholarly journals Genotyping of Eight Human Platelet Antigen Systems in Serbian Blood Donors: Foundation for Platelet Apheresis Registry

2021 ◽  
pp. 1-6
Author(s):  
Snezana Jovanovic Srzentic ◽  
Marko Lilic ◽  
Natasa Vavic ◽  
Ivana Radovic ◽  
Iva Djilas
Keyword(s):  
Signal Detection ◽  
Blood Donors ◽  
Human Platelet ◽  
Allele Frequencies ◽  
Successful Implementation ◽  
Genotype Frequencies ◽  
Platelet Antigen ◽  
Human Platelet Antigen ◽  
Specific Amplification ◽  
Compatible Blood

Introduction: The aim of this study was to investigate the allele and genotype frequencies of 8 human platelet antigen (HPA) systems among blood donors from the Blood Transfusion Institute of Serbia and to compare them with published studies. These data would be useful to establish the basis for a platelet apheresis donor registry. Material and Methods: Seventy-two unrelated male platelet apheresis/blood donors from Serbia were typed for 8 HPA systems (HPA-1 to HPA-6, HPA-9, and HPA-15) via the FluoGene method, based on polymerase chain reaction-sequence-specific amplification (PCR-SSP; PCR using sequence-specific primers) with fluorometric signal detection. Allele and genotype frequencies were estimated by direct counting and compared to the expected genotype frequencies according to the Hardy-Weinberg principle. The transfusion mismatch probability was calculated for every HPA specificity. Results: The allele frequencies were: HPA-1a, 0.868; HPA-1b, 0.132; HPA-2a, 0.917; HPA-2b, 0.083; HPA-3a, 0.611; HPA-3b, 0.389; HPA-5a, 0.903; HPA-5b, 0.097; HPA-9a, 0.993; HPA-9b, 0.007; HPA-15a, 0.472; and HPA-15b, 0.528. For HPA-4 and HPA-6 only allele a was detected. Discussion: The HPA allele frequencies of European populations showed no significant differences in comparison with our results. Statistically significant differences were revealed in comparison with some populations of non-European origin. In the tested donors no HPA-2 bb genotype was detected, but we found 1 donor with the rare HPA-9b allele. The biggest transfusion mismatch probability in the Serbian population is for systems HPA-15 (37.4%) and HPA-3 (36.2%), which means that more than a third of random transfusions could cause mismatch in these systems. This study was enabled by the introduction of molecular HPA typing, and it provides initial results of the HPA allele and genotype frequencies in the population of blood donors in Serbia. They will be used to provide a compatible blood supply on demand for treating patients with alloimmune thrombocytopenic disorders. The successful implementation of PCR-SSP with fluorometric signal detection could be further complemented in the future by the introduction of high-throughput methods, which will largely depend on the available financial resources.

Human Platelet Antigen 3 (HPA-3): Localization of the Determinant of the Alloantibody Leka (HPA-3a) to the C-Terminus of Platelet Glycoprotein IIb Heavy Chain and Contribution of O-Linked Carbohydrates

1993 ◽  
Vol 69 (05) ◽  
pp. 485-489 ◽  
Author(s):  
Isabelle Djaffar ◽  
Didier Vilette ◽  
Dominique Pidard ◽  
Jean-Luc Wautier ◽  
Jean-Philippe Rosa
Keyword(s):  
Amino Acids ◽  
Heavy Chain ◽  
Human Platelet ◽  
Platelet Glycoprotein ◽  
Fibrinogen Receptor ◽  
C Terminus ◽  
Platelet Antigen ◽  
Human Platelet Antigen ◽  
Determinant Structure ◽  
Polymerase Chain

SummaryThe human platelet antigen (HPA) 3 system is expressed on GPIIb, one subunit of GPIIb-IIIa, the platelet fibrinogen receptor. It was recently shown that HPA-3 was associated with an Ile843/Ser polymorphism. To investigate further HPA-3 determinant structure, we localized an HPA-3a determinant, recognized by the alloantiserum Leka, within the last 29 amino acids of GPIIbα. This region encompasses the polymorphic Ile843, which, as expected, is substituted into Ser in Leka-negative individuals, as shown by DNA sequence after polymerase chain reaction on platelet RNA. In addition, contribution of glycosylation to the determinant structure was demonstrated since the Leka antigenicity was strongly decreased after specifically removing nonterminal O-linked sugars, but not terminal sialic acids. We have thus refined the localization of an HPA-3a determinant within the last 29 amino acids, including Ile843, of GPIIb heavy chain, and shown that the Leka HPA-3a determinant is dependent, in part, upon the serine-linked carbohydrates adjacent to Ile/Ser843.

The Human Platelet Antigen HPA-la/lb (PIA1/PIA2)Polymorphism and Cerebral Ischaemia

1997 ◽  
Vol 78 (02) ◽  
pp. 964-965 ◽  
Author(s):  
Karl H Reuner ◽  
Michael Elgas ◽  
Michael Kaps ◽  
Andreas Ruf ◽  
Heinrich Patscheke
Keyword(s):  
Cerebral Ischaemia ◽  
Human Platelet ◽  
Platelet Antigen ◽  
Human Platelet Antigen

scholarly journals Human Platelet Antigen Genotyping and Expression of CD109 (Human Platelet Antigen 15) mRNA in Various Human Cell Types

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Sang Mee Hwang ◽  
Mi Jung Kim ◽  
Ho Eun Chang ◽  
Yun Ji Hong ◽  
Taek Soo Kim ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Human Platelet ◽  
Human Fibroblasts ◽  
Leukemia Cell ◽  
Embryonic Stem ◽  
Melting Curve ◽  
Leukemia Cell Line ◽  
Melting Curve Analysis ◽  
Platelet Antigen ◽  
Human Platelet Antigen

CD109 gene encodes a glycosylphosphatidylinositol-linked glycoprotein found in a subset of platelets and endothelial cell, and human platelet antigen (HPA) 15 is found on CD109. We evaluated the HPA genotype and/or the CD109 mRNA expression on two peripheral blood stem cells (PBSC), two peripheral bloods (PB), 12 granulocyte products, natural killer (NK)-92, B-lymphocyte (CO88BV59-1), K-562 leukemia cell line, human embryonic stem cell (hESC), and human fibroblasts (HF). HPA genotyping was performed by SNaPshot assay and CD109 mRNA expression was evaluated by real-time PCR with SYBR green and melting curve analysis. Genotype HPA-15a/-15a was found in PBSC#1 and two granulocyte products, and HPA-15a/-15b was found in PBSC#2, eight granulocyte products, NK-92, K-562, hESC, and HF, and HPA-15b/-15b was found in two granulocyte products. CD109 mRNA expression was highly increased in HF and increased in CD34+ and CD34− PBSCs and some granulocyte products, compared to the PB. However, the increase of expression level varied among the PBSC and granulocyte products. The CD109 mRNA expression of NK-92, K-562, hESC, and CO 88BV59-1 was not detected. HPA genotype was evaluated in various cells and the expression of CD109, which contains HPA 15, was different among cell lines and high in HF and PBSCs.

scholarly journals Rapid Determination of Lymphogranuloma Venereum Serovars of Chlamydia trachomatis by Quantitative High-Resolution Melt Analysis (HRMA)

2012 ◽  
Vol 50 (11) ◽  
pp. 3751-3753 ◽  
Author(s):  
J. Twin ◽  
M. P. Stevens ◽  
S. M. Garland ◽  
A. M. Zaia ◽  
S. N. Tabrizi
Keyword(s):  
High Resolution ◽  
Chlamydia Trachomatis ◽  
Rapid Determination ◽  
Lymphogranuloma Venereum ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis

Incidence of transfusion-induced platelet-reactive antibodies evaluated by specific assays for the detection of human leucocyte antigen and human platelet antigen antibodies

Vox Sanguinis ◽  
2007 ◽  
Vol 93 (3) ◽  
pp. 241-249 ◽  
Author(s):  
R. Fontão-Wendel ◽  
L. C. N. Silva ◽  
C. B. R. Saviolo ◽  
B. Primavera ◽  
S. Wendel
Keyword(s):  
Human Leucocyte Antigen ◽  
Human Platelet ◽  
Platelet Antigen ◽  
Human Platelet Antigen

scholarly journals High-resolution melt analysis to detect sequence variations in highly homologous gene regions: application to CYP2B6

2013 ◽  
Vol 14 (8) ◽  
pp. 913-922 ◽  
Author(s):  
Greyson P Twist ◽  
Roger Gaedigk ◽  
J Steven Leeder ◽  
Andrea Gaedigk
Keyword(s):  
High Resolution ◽  
Homologous Gene ◽  
High Resolution Melt ◽  
High Resolution Melt Analysis ◽  
Sequence Variations

Human platelet antigen allele frequencies and new mutations on platelet glycoprotein genes in the Chinese Han population

2011 ◽  
Vol 21 (5) ◽  
pp. 330-337 ◽  
Author(s):  
X. Xu ◽  
Y. Liu ◽  
Y. Ying ◽  
S. Tao ◽  
X. Hong ◽  
...  
Keyword(s):  
Human Platelet ◽  
Allele Frequencies ◽  
Chinese Han Population ◽  
Platelet Glycoprotein ◽  
Chinese Han ◽  
Han Population ◽  
Platelet Antigen ◽  
Human Platelet Antigen ◽  
New Mutations
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