Canine mammary carcinomas: influence of histological grade, vascular invasion, proliferation, microvessel density and VEGFR2 expression on lymph node status and survival time

Elective cervical lymphadenectomy often is performed for laryngeal carcinoma to eliminate metastatic disease that escapes clinical and radiographic detection. We investigated characteristics of the primary tumor that might predict cervical lymph node status. We obtained archival tissue from 88 laryngectomies—65 with concurrent cervical lymphadenectomies. Of the 40 clinically negative necks that were dissected, 17% showed lymph node metastasis by pathologic examination. The primary tumors were examined immunohistochemically for expression of epidermal growth factor receptor (EGFR), p53, cathepsin D, proliferating cell nuclear antigen (PCNA), and Ki-67–specific antigen, and by flow cytometry for DNA ploidy—cell cycle analysis. Seventy-seven percent of the cases showed aberrant p53 staining, 99% expressed EGFR, 40% produced cathepsin D, 29% were aneuploid, and 54% had a moderate or high synthesis phase fraction (SPF). High grade, aneuploidy, and tumor vascular invasion independently predicted cervical node metastasis (p < .04 each). Supraglottic locale (p < .16) and a raggedly infiltrating invading margin (p < .13) were weakly associated with node positivity. Advanced clinical T status, the expression of EGFR, p53, and cathepsin D, the PCNA and Ki-67 indices, and SPF did not correlate with node metastasis. The presence of cervical node metastasis predicted poor disease-free (p < .005) and overall survival (p < .04). Advanced clinical T status correlated with brief overall survival (p < .02). Tumor site, histopathologic parameters, ploidy, SPF, PCNA and Ki-67 indices, and the expression of p53, EGFR, and cathepsin D did not affect survival. The presence of vascular invasion, high grade, and aneuploidy may help identify which patients would benefit from elective cervical lymphadenectomy. The correlation of cervical lymph node status and clinical T category with survival confirms the results of previous studies.

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pS2 EXPRESSION IN INFILTRATING DUCTAL CARCINOMA OF THE BREAST CORRELATES WITH OESTROGEN RECEPTOR POSITIVITY BUT NOT WITH HISTOLOGICAL GRADE AND LYMPH NODE STATUS

Pathology ◽

10.1080/00313020120062848 ◽

2001 ◽

Vol 33 (3) ◽

pp. 283-286

Author(s):

Lai-Meng Looi ◽

Wy Wan Azura ◽

Phaik-Leng Cheah ◽

Min-Hwei Ng

Keyword(s):

Lymph Node ◽

Oestrogen Receptor ◽

Ductal Carcinoma ◽

Histological Grade ◽

Lymph Node Status ◽

Carcinoma Of The Breast ◽

Infiltrating Ductal Carcinoma ◽

Node Status

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Stromal expression of CD10 in breast carcinoma and its correlation with clinicopathological parameters

South Asian Journal of Cancer ◽

10.4103/sajc.sajc_56_18 ◽

2019 ◽

Vol 08 (01) ◽

pp. 18-21

Author(s):

Ashish Nitin Dhande ◽

Siddhi Gaurish Sinai Khandeparkar ◽

Avinash R. Joshi ◽

Maithili Mandar Kulkarni ◽

Nidhi Pandya ◽

...

Keyword(s):

Lymph Node ◽

Histological Grade ◽

Tumor Grade ◽

Clinicopathological Parameters ◽

Lymph Node Status ◽

Breast Carcinomas ◽

Independent Prognostic Marker ◽

Immunohistochemical Markers ◽

Node Status ◽

Cd10 Expression

Abstract Introduction: Breast cancer is the foremost cause of death in women worldwide with more than one million cases occurring annually. Aim: This study was conducted to study the stromal CD10 expression in breast carcinomas (BCa) and its correlation with various prognostic factors such as tumor size, histological grade, lymph node status, estrogen receptor (ER), progesterone receptor (PR), HER2neu, and Ki67 status. Materials and Methods: Sixty cases of BCa diagnosed between 2013 and 2015 were included in the study. Stromal expression of CD10 was studied on entire section of selected BCa blocks for all cases. A technique of manual tissue microarray was employed for the analysis of expression of immunohistochemical markers ER, PR, and HER2/Neu and Ki67 in all cases. Results were subjected to statistical analysis. Results: Stromal CD10 positivity was seen in 78.3% cases, out of which 53.3% of cases were strongly positive, and 25.0% cases were weakly positive. Positivity for ER, PR, HER2, and Ki67 was 31.7%, 33.3%, 65%, and 75%, respectively. Stromal expression of CD10 was found to be significantly associated with increasing tumor grade, lymph node status, HER2neu positivity, ER negativity, and Ki67 positivity. CD10 stromal expression was seen mainly in PR negative BCa cases; however, it was statistically insignificant. It was noted that CD10 stromal positivity increased with increasing grade. Conclusion: CD10 can be used as an independent prognostic marker and should be included in routine histopathology report. CD10 could act as a potential target for newer drug development.

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