Proteomic analysis of aqueous humor in canine primary angle‐closure glaucoma in American Cocker Spaniel dogs

2021 ◽  
Author(s):  
Seongjin Yun ◽  
Dabin Lee ◽  
Seonmi Kang ◽  
Dong Wook Kim ◽  
Youngsam Kim ◽  
...  
Keyword(s):  
Aqueous Humor ◽  
Proteomic Analysis ◽  
Angle Closure Glaucoma ◽  
Angle Closure ◽  
Cocker Spaniel ◽  
Primary Angle Closure ◽  
Primary Angle Closure Glaucoma

Aqueous humor protein dysregulation in primary angle-closure glaucoma

2018 ◽  
Vol 39 (4) ◽  
pp. 861-871 ◽  
Author(s):  
Sunil S. Adav ◽  
Jin Wei ◽  
Jingru Qian ◽  
Nicola Yi’An Gan ◽  
Leonard W. L. Yip ◽  
...  
Keyword(s):  
Aqueous Humor ◽  
Angle Closure Glaucoma ◽  
Angle Closure ◽  
Primary Angle Closure ◽  
Primary Angle Closure Glaucoma

Evaluation of matrix metalloproteinases and tissue inhibitors of metalloproteinases in aqueous humor of dogs with versus without naturally occurring primary angle-closure glaucoma

2021 ◽  
pp. 1-11
Author(s):  
Stephanie A. Pumphrey ◽  
Emily Zitek-Morrison ◽  
Stefano Pizzirani ◽  
Dawn M. Meola
Keyword(s):  
Extracellular Matrix ◽  
Matrix Metalloproteinases ◽  
Aqueous Humor ◽  
Late Stage ◽  
Angle Closure Glaucoma ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Angle Closure ◽  
Primary Angle Closure ◽  
Primary Angle Closure Glaucoma ◽  
Naturally Occurring

Abstract OBJECTIVE To compare concentrations of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in aqueous humor from ophthalmologically normal dogs and dogs with naturally occurring primary angle-closure glaucoma (cPACG). SAMPLE Aqueous humor samples from 12 eyes with cPACG and 18 ophthalmologically normal eyes of dogs. PROCEDURES A multiplex fluorescence-based ELISA was used to measure concentrations of MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, and TIMP-4. Results for eyes with versus without cPACG were compared. RESULTS Significantly higher mean concentrations of MMP-1 (45% higher), MMP-2 (55% higher), MMP-3 (39% higher), MMP-8 (79% higher), MMP-9 (29% higher), MMP-10 (60% higher), TIMP-1 (63% higher), and TIMP-2 (136% higher) were detected in aqueous humor from eyes with cPACG, compared with ophthalmologically normal eyes. CLINICAL RELEVANCE MMPs and TIMPs have pivotal roles in extracellular matrix turnover and homeostasis in the outflow pathways of the eye. Results of the present study documented higher concentrations of MMPs and TIMPs in aqueous humor samples from dog eyes with late-stage cPACG. Although, to our knowledge, TIMPs have not previously been evaluated in the context of cPACG, the markedly higher concentration of TIMPs in eyes with cPACG suggested that inhibition of proteolysis and extracellular matrix turnover might be a factor in the development of glaucoma in susceptible individuals. However, because the present study used samples from dogs with late-stage cPACG, further work is required to characterize the temporal relationship between MMP and TIMP concentration changes and onset or progression of disease.

Multiplex Cytokine Analysis of Aqueous Humor from the Patients with Chronic Primary Angle Closure Glaucoma

2017 ◽  
Vol 42 (12) ◽  
pp. 1608-1613 ◽  
Author(s):  
Roopam Duvesh ◽  
George Puthuran ◽  
Kavitha Srinivasan ◽  
Venkatesh Rengaraj ◽  
SR Krishnadas ◽  
...  
Keyword(s):  
Aqueous Humor ◽  
Angle Closure Glaucoma ◽  
Angle Closure ◽  
Primary Angle Closure ◽  
Primary Angle Closure Glaucoma ◽  
Cytokine Analysis ◽  
Multiplex Cytokine

Does Spontaneous Blinking Increase Iris-Lens Contact?

2010 ◽  
Author(s):  
Rouzbeh Amini ◽  
Victor H. Barocas
Keyword(s):  
Aqueous Humor ◽  
Angle Closure Glaucoma ◽  
The Other ◽  
Abnormal Behavior ◽  
Angle Closure ◽  
Constant Flow ◽  
Primary Angle Closure ◽  
Primary Angle Closure Glaucoma ◽  
Outflow Pathway

The constant flow of aqueous humor (AH) in the anterior eye provides avascular tissues, notably the lens and cornea, with oxygen and nutrients (Fig. 1a). The hydrodynamics of AH and the iris contour are directly related to each other and abnormality of one may cause abnormal behavior in the other one. For example, in primary angle closure glaucoma, the abnormal anteriorly-located iris blocks the outflow pathway of AH. Increased AH pressure, in return, may push the iris more towards the anterior and worsen the blockage.

Novel inflammatory cytokines (IL-36, 37, 38) in the aqueous humor from patients with chronic primary angle closure glaucoma

2019 ◽  
Vol 71 ◽  
pp. 164-168 ◽  
Author(s):  
Jin-ling Zhang ◽  
Xiang-yuan Song ◽  
Ya-ying Chen ◽  
Thi Hoang Anh Nguyen ◽  
Jing-yi Zhang ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Aqueous Humor ◽  
Angle Closure Glaucoma ◽  
Angle Closure ◽  
Primary Angle Closure ◽  
Primary Angle Closure Glaucoma

scholarly journals Inflammatory cytokine profiles in eyes with primary angle-closure glaucoma

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Yayi Wang ◽  
Shida Chen ◽  
Yaoming Liu ◽  
Wenbin Huang ◽  
Xinyi Li ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Aqueous Humor ◽  
Clinical Information ◽  
Cross Sectional Study ◽  
Angle Closure Glaucoma ◽  
Angle Closure ◽  
Cross Sectional ◽  
Primary Angle Closure ◽  
Primary Angle Closure Glaucoma ◽  
Inflammatory Protein

Acute primary angle-closure (APAC) eyes show an early ‘acute inflammatory’ condition, while the inflammation condition has not been fully elucidated in the development of primary angle-closure glaucoma (PACG). To evaluate the roles of inflammatory cytokines in the pathogenesis of PACG, this cross-sectional study involved 40 eyes of 32 PACG patients who required trabeculectomy and 24 eyes of 24 patients who required cataract surgery. The aqueous humor samples were collected at the time of surgery. Fifteen inflammatory cytokines were detected using the multiplex bead immunoassay technique, and the clinical information was recorded for the correlation analysis. Eight of the 15 cytokines were all detectable in both groups, including granulocyte colony-stimulating factor (G-CSF), interleukin (IL)-6, IL-8, monocyte chemotactic protein (MCP)-1, MCP-3, macrophage-derived chemokine (MDC), macrophage inflammatory protein (MIP)-1β, and vascular endothelial growth factor (VEGF). When compared with the cataract patients, the MCP-3, MDC, and VEGF levels were elevated in the PACG patients, while the MCP-1 and MIP-1β levels were decreased. However, the G-CSF, IL-6, and IL-8 levels were similar between the two groups. The MCP-1 concentration was elevated accordingly as the disease progressed in the PACG patients. Our results suggest the PACG eyes retained a ‘mild inflammation’ condition in the aqueous humor, and MCP-1 may play an important role in the progression of this disease.

Primary angle-closure glaucoma in young people

2016 ◽  
Vol 3 (4) ◽  
pp. 237-247
Author(s):  
Maria Hanna Niżankowska
Keyword(s):  
Young People ◽  
Angle Closure Glaucoma ◽  
Angle Closure ◽  
Primary Angle Closure ◽  
Primary Angle Closure Glaucoma
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