Electrophoretic Migration of Organophosphorus Pesticides as a Function of Separation Potential in Micellar Electrokinetic Chromatography: A Basis of Predicting the Retention Factors

2012 ◽  
Author(s):  
Wan Aini Wan Ibrahim ◽  
S. M. Monjurul Alam ◽  
A. B. Sulaiman
Keyword(s):  
Key Words ◽  
Linear Model ◽  
Micellar Electrokinetic Chromatography ◽  
Functional Relationship ◽  
Organophosphorus Pesticides ◽  
Electrokinetic Chromatography ◽  
Separation Potential ◽  
Retention Factors ◽  
Hydrophobic Compounds ◽  
Alternative Approach

Dalam kromatografi elektrokinetik misel, faktor penahanan (k´) biasanya dikira menggunakan mobiliti misel penanda hidrofobik yang tertahan sepenuhnya dan penanda ini tidak banyak didapati untuk kegunaan universal. Satu pendekatan alternatif dicadangkan di sini untuk meramalkan nilai k´ berdasarkan fungsi hubungan antara masa penahanan zat terlarut dan keupayaan pemisahan menggunakan pestisid organofosforus sebagai sebatian rujukan hidrofobik. Penggunaan model linear yang ringkas ini dinilai untuk satu set pestisid organofosforus hidrofilik lain. Kata kunci: Kromatografi elektrokinetik misel (MEKC), OPPs hidrofobik, OPPs hidrofilik Retention factors (k´) in Micellar Electrokinetic Chromatography (MEKC) are very often calculated against the mobility of fully retained hydrophobic micelle markers, which are not abundant for universal use. An alternative approach was proposed to predict the k´ values on the basis of a functional relationship between the solutes retention times and separation potentials using organophosphorus pesticides (OPPs) as reference hydrophobic compounds. The use of the proposed simple linear model was evaluated for another set of hydrophilic OPPs and its practicality was defined. Key words: Micellar Electrokinetic Chromatography (MEKC), hydrophobic OPPs, hydrophilic OPPs

Separation of Organophosphorus Pesticides Using Micellar Electrokinetic Chromatography

2012 ◽  
Author(s):  
W. Aini W. Ibrahim ◽  
S. M. Monjurul Alam ◽  
Azli Sulaiman
Keyword(s):  
Key Words ◽  
Sodium Dodecyl Sulphate ◽  
Micellar Electrokinetic Chromatography ◽  
Organophosphorus Pesticides ◽  
Sodium Dodecyl ◽  
Borate Buffer ◽  
Electrokinetic Chromatography ◽  
Organic Modifier ◽  
Strong Peak ◽  
Sds Surfactant

Pemisahan enam pestisid organofosforus (mevinfos, metidation, diazinon, prefenofos, kuinalfos dan klorpirifos) telah dikaji menggunakan teknik kromatografi elektrokinetik misel (MEKC) dengan pengesan Ultralembayung–Nampak. Proses pemisahan dilakukan dengan menggunakan surfaktan anionik natrium dodesil sulfat (SDS) dan larutan penimbal borat atau fosfat sebagai elektrolit pembawa. Pemisahan yang dilakukan didapati tidak lengkap walaupun telah dilakukan perubahan terhadap pelbagai faktor seperti jenis larutan penimbal, kepekatan surfaktan dan larutan penimbal, penambahan pengubahsuai metanol dan juga pengubahsuaian pH larutan penimbal yang digunakan. Tanpa penggunaan dan pengubahsuaian faktor di atas, pestisid mevinfos sahaja selalu muncul sebagai puncak yang tajam diikuti oleh diazinon dan metidation. Walaupun tanpa kehadiran surfaktan SDS dalam matrik larutan penimbal, mevinfos dan diazinon dapat dikesan manakala pestisid OPPs yang lainnya tidak dapat dikesan. Penggunaan β–siklodekstrin (β–CD) 6 mM, penimbal borat 20 mM dengan SDS 40 mM dan pH penimbal 9.5 memberikan keputusan terbaik bagi pemisahan pestisid mevinfos, diazinon dan metidation. Keputusan dalam kajian ini mencerminkan kaitan antara keterlarutan dan sifat hidrofobik pestisid. Kata kunci: Pestisid organofosforus; kromatografi elektrokinetik misel; natrium dodesil sulfat; siklodekstrin; ultralembayung-Nampak Separation of six organophosphorus pesticides (mevinphos, methidathion, diazinon, profenofos, quinalphos and chlorpyrifos) has been investigated using micellar electrokinetic chromatography (MEKC) with on–column UV–Vis detection. Separations involved using anionic sodium dodecyl sulphate (SDS) surfactant and phosphate or borate buffer as carrier electrolytes. Separations have been found incomplete regardless of changing all sort of possible factors such as buffer types, buffer and surfactant concentrations, addition of organic modifier, methanol and pH of the running buffer. Irrespective of the combinations of levels of factors, mevinphos alone appeared always as a distinguishable strong peak followed by diazinon and methidathion. Even in the absence of any surfactant in buffer matrices, mevinphos and diazinon were detected but methidathion, profenofos, quinalphos and chlorpyrifos were undetected. The use of 6 mM β–cyclodextrin (β–CD), 20 mM borate buffer with 40 mM SDS and buffer pH of 9.5 resulted in the best separation of mevinphos, diazinon and methidathion. The results found in this study might reflect the solubility and hydrophobicity properties of the pesticides. Key words: Organophosphorus Pesticides (OPPs); Micellar electrokinetic chromatography; Sodium dodecy sulphate; cyclodextrin; UV-Vis

scholarly journals Separation of hydrophobic compounds by nonaqueous micellar electrokinetic chromatography using formamide solvent.

1999 ◽  
Vol 48 (2) ◽  
pp. 239-244 ◽  
Author(s):  
Masamitsu NAKAGAWA ◽  
Jin-Ming LIN ◽  
Tatsuro NAKAGAMA ◽  
Katsumi UCHIYAMA ◽  
Toshiyuki HOBO
Keyword(s):  
Micellar Electrokinetic Chromatography ◽  
Electrokinetic Chromatography ◽  
Hydrophobic Compounds

scholarly journals Bile Salts in Chiral Micellar Electrokinetic Chromatography: 2000–2020

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5531
Author(s):  
Raymond B. Yu ◽  
Joselito P. Quirino
Keyword(s):  
Bile Salts ◽  
Micellar Electrokinetic Chromatography ◽  
Electrokinetic Chromatography ◽  
Chiral Selectors ◽  
Hydrophobic Compounds ◽  
Naturally Occurring ◽  
Partial Filling ◽  
Single Drop Microextraction ◽  
Potential Applications ◽  
Chiral Surfactants

Bile salts are naturally occurring chiral surfactants that are able to solubilize hydrophobic compounds. Because of this ability, bile salts were exploited as chiral selectors added to the background solution (BGS) in the chiral micellar electrokinetic chromatography (MEKC) of various small molecules. In this review, we aimed to examine the developments in research on chiral MEKC using bile salts as chiral selectors over the past 20 years. The review begins with a discussion of the aggregation of bile salts in chiral recognition and separation, followed by the use of single bile salts and bile salts with other chiral selectors (i.e., cyclodextrins, proteins and single-stranded DNA aptamers). Advanced techniques such as partial-filling MEKC, stacking and single-drop microextraction were considered. Potential applications to real samples, including enantiomeric impurity analysis, were also discussed.

Separation of highly hydrophobic compounds by cyclodextrin-modified micellar electrokinetic chromatography

1990 ◽  
Vol 516 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Shigeru Terabe ◽  
Yosuke Miyashita ◽  
Osamu Shibata ◽  
Elizabeth R. Barnhart ◽  
Louis R. Alexander ◽  
...  
Keyword(s):  
Micellar Electrokinetic Chromatography ◽  
Electrokinetic Chromatography ◽  
Hydrophobic Compounds ◽  
Highly Hydrophobic
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