scholarly journals A novel method for recording whole-cell and single-channel currents from differentiating cerebellar granule cells in situ

1994 ◽  
Vol 79 (4) ◽  
pp. 495-504 ◽  
Author(s):  
E Radden ◽  
M Behrens ◽  
FW Pehlemann ◽  
J Schmidtmayer
Keyword(s):  
Granule Cells ◽  
Single Channel ◽  
Cerebellar Granule Cells ◽  
Whole Cell ◽  
Cerebellar Granule ◽  
Novel Method ◽  
Single Channel Currents ◽  
Channel Currents

Single-channel and whole-cell currents in rat cerebellar granule cells

1990 ◽  
Vol 535 (1) ◽  
pp. 33-38 ◽  
Author(s):  
T. Jalonen ◽  
S. Johansson ◽  
I. Holopainen ◽  
S.S. Oja ◽  
P. A˚rhem
Keyword(s):  
Granule Cells ◽  
Single Channel ◽  
Cerebellar Granule Cells ◽  
Whole Cell ◽  
Cerebellar Granule

scholarly journals Triheteromeric GluN1/GluN2A/GluN2C NMDARs with Unique Single-Channel Properties Are the Dominant Receptor Population in Cerebellar Granule Cells

Neuron ◽  
2018 ◽  
Vol 99 (2) ◽  
pp. 315-328.e5 ◽  
Author(s):  
Subhrajit Bhattacharya ◽  
Alpa Khatri ◽  
Sharon A. Swanger ◽  
John O. DiRaddo ◽  
Feng Yi ◽  
...  
Keyword(s):  
Granule Cells ◽  
Single Channel ◽  
Cerebellar Granule Cells ◽  
Cerebellar Granule ◽  
Channel Properties

scholarly journals Stretch-activated whole cell currents in adult rat cardiac myocytes

2000 ◽  
Vol 278 (2) ◽  
pp. H548-H557 ◽  
Author(s):  
Tao Zeng ◽  
Glenna C. L. Bett ◽  
Frederick Sachs
Keyword(s):  
Single Channel ◽  
Ventricular Myocytes ◽  
Reversal Potential ◽  
Cell Voltage ◽  
Cellular Level ◽  
Whole Cell ◽  
Adult Rat ◽  
Longitudinal Stretch ◽  
Single Channel Currents ◽  
Channel Currents

Mechanoelectric transduction can initiate cardiac arrhythmias. To examine the origins of this effect at the cellular level, we made whole cell voltage-clamp recordings from acutely isolated rat ventricular myocytes under controlled strain. Longitudinal stretch elicited noninactivating inward cationic currents that increased the action potential duration. These stretch-activated currents could be blocked by 100 μM Gd3+ but not by octanol. The current-voltage relationship was nearly linear, with a reversal potential of approximately −6 mV in normal Tyrode solution. Current density varied with sarcomere length (SL) according to I (pA/pF) = 8.3 − 5.0SL (μm). Repeated attempts to record single channel currents from stretch-activated ion channels failed, in accord with the absence of such data from the literature. The inability to record single channel currents may be a result of channels being located on internal membranes such as the T tubules or, possibly, inactivation of the channels by the mechanics of patch formation.

A microscope stage temperature controller for the study of whole-cell or single-channel currents

1985 ◽  
Vol 404 (4) ◽  
pp. 374-377 ◽  
Author(s):  
Lee D. Chabala ◽  
Robert E. Sheridan ◽  
David C. Hodge ◽  
John N. Power ◽  
Michael P. Walsh
Keyword(s):  
Single Channel ◽  
Whole Cell ◽  
Temperature Controller ◽  
Single Channel Currents ◽  
Channel Currents ◽  
Microscope Stage
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