Imaging ex vivo and in vitro brain morphology in animal models with ultrahigh resolution optical coherence tomography

2004 ◽  
Vol 9 (4) ◽  
pp. 719 ◽  
Author(s):  
Kostadinka Bizheva ◽  
Angelika Unterhuber ◽  
Boris Hermann ◽  
Boris Považay ◽  
Harald Sattmann ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Animal Models ◽  
Ex Vivo ◽  
Brain Morphology ◽  
Optical Coherence ◽  
Ultrahigh Resolution

Abstract WP1: High-Frequency Optical Coherence Tomography for Cerebrovascular Disease

Stroke ◽  
2020 ◽  
Vol 51 (Suppl_1) ◽  
Author(s):  
Ajit S Puri ◽  
Giovanni Ughi ◽  
Robert M King ◽  
Matthew Gounis
Keyword(s):  
Optical Coherence Tomography ◽  
Cerebrovascular Disease ◽  
High Frequency ◽  
Ex Vivo ◽  
Thrombus Formation ◽  
Image Resolution ◽  
Patient Specific ◽  
Optical Coherence

Introduction: Optical coherence tomography (OCT) has played an important role in the diagnosis and treatment guidance in coronary artery disease. However, existing OCT systems are not suitable for routine neurovascular applications due to the size and tortuosity of the arteries. Hypothesis: We seek to demonstrate a prototype high-frequency OCT (HF-OCT) capable of high-resolution imaging in simulated cerebrovascular anatomy. Methods: A low-profile HF-OCT system was constructed with an image resolution approaching 10μm. Using an in vitro, patient-specific model of the circle of Willis with circulating porcine blood, we characterized the delivery of the device and ability to image in a tortuous path. Also, human cadaver intracranial atherosclerosis plaques were imaged with HF-OCT and assessed by an expert imager. Finally, neurovascular devices were implanted in 8 pigs (Fig 1) and HF-OCT imaging was compared with gold-standard DSA and CT. Results: In the phantom, optimal blood clearance was achieved through an intermediate catheter (5 Fr Navien) with infusion of contrast at 5 ml/s in the internal carotid and basilar artery, and 3 ml/sec in the MCA. The in vivo study demonstrated that both malapposition of devices or thrombus formation along the device surface could be reliably diagnosed among 3 reviewers (Fleiss’s kappa of 0.87 and 0.9, respectively). This agreement was superior to DSA and CT. Imaging in tortuous swine brachial showed in all cases imaging free of artifacts, uniform illumination and ability to visualize vessel wall layers. Plaque types including ‘lipid pools’, fibrotic, and calcific tissue from cadaver specimens of ICAD could be adequately depicted by HF-OCT. Conclusion: In vitro, in vivo and ex vivo characterization of a novel HF-OCT device has shown it is capable of imaging in the tortuous intracranial vascular anatomy. This technology has to potential to aid in the diagnosis of cerebrovascular disease and guide optimal endovascular treatment.

Imaging brain morphology with ultrahigh-resolution optical coherence tomography

2003 ◽  
Author(s):  
Kostadinka K. Bizheva ◽  
Angelika Unterhuber ◽  
Boris Hermann ◽  
Boris Povazay ◽  
Harald Sattmann ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Brain Morphology ◽  
Optical Coherence ◽  
Ultrahigh Resolution

Imaging brain morphology with ultrahigh resolution optical coherence tomography

2003 ◽  
Author(s):  
Kostadinka Bizheva ◽  
Angelika Unterhuber ◽  
Boris Hermann ◽  
Boris Považay ◽  
Michael Mei ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Brain Morphology ◽  
Optical Coherence ◽  
Ultrahigh Resolution

scholarly journals A method to determine the radial compliance of porcine coronary arteries ex vivo via optical coherence tomography

2020 ◽  
Vol 6 (3) ◽  
pp. 48-51
Author(s):  
Christoph Brandt-Wunderlich ◽  
Franziska Bonin ◽  
Wolfram Schmidt ◽  
Niels Grabow ◽  
Klaus- Peter Schmitz ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Coronary Arteries ◽  
Vascular Tissue ◽  
Ex Vivo ◽  
Imaging Method ◽  
In Vitro Test ◽  
Optical Coherence ◽  
Porcine Coronary Artery ◽  
Vitro Test

AbstractOptical coherence tomography (OCT) as imaging method is widely used in ophthalmology, oncology and cardiology. For intravascular imaging the OCT is used for pre-interventional as well as post-procedural assessments. Within the current study a test setup for ex vivo determination of the compliance of porcine coronary arteries via OCT is described. Diameter measurements based on OCT imaging were performed during consecutive pressurization of a porcine coronary artery from 40 to 200 mmHg in a physiological environment. The test results indicate that the radial compliance depends on the specific segment of the artery as well as the pressure range considered. The revealed compliance data can be used for numerical simulations of the vascular tissue as well as for optimization of in vitro test setups for pulsatile testing of vascular implants.

Visualisation of distribution of gold nanoparticles in liver tissues ex vivo and in vitro using the method of optical coherence tomography

2012 ◽  
Vol 42 (6) ◽  
pp. 478-483 ◽  
Author(s):  
Elina A Genina ◽  
G S Terentyuk ◽  
B N Khlebtsov ◽  
A N Bashkatov ◽  
Valerii V Tuchin
Keyword(s):  
Gold Nanoparticles ◽  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Optical Coherence ◽  
Liver Tissues

In vitro measurement of rabbit corneal epithelial thickness using ultrahigh resolution optical coherence tomography

2005 ◽  
Vol 8 (2) ◽  
pp. 85-88 ◽  
Author(s):  
Bibiana J. Reiser ◽  
Teresa S. Ignacio ◽  
Yimin Wang ◽  
Mehran Taban ◽  
Jordan M. Graff ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Ultrahigh Resolution ◽  
Corneal Epithelial ◽  
Epithelial Thickness ◽  
Corneal Epithelial Thickness

Ultrahigh-resolution and 3-dimensional optical coherence tomography ex vivo imaging of the large and small intestines

2005 ◽  
Vol 62 (4) ◽  
pp. 561-574 ◽  
Author(s):  
Pei-Lin Hsiung ◽  
Liron Pantanowitz ◽  
Aaron D. Aguirre ◽  
Yu Chen ◽  
Darshan Phatak ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Optical Coherence ◽  
Ultrahigh Resolution ◽  
3 Dimensional ◽  
Small Intestines ◽  
Ex Vivo Imaging

Dental optical coherence tomography: a comparison of two in vitro systems

1999 ◽  
Vol 29 (2) ◽  
pp. 85-89 ◽  
Author(s):  
L L Otis ◽  
B W Colston ◽  
M J Everett ◽  
H Nathel
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
In Vitro Systems

scholarly journals Morpho-molecular ex vivo detection and grading of non-muscle-invasive bladder cancer using forward imaging probe based multimodal optical coherence tomography and Raman spectroscopy

The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1445-1456 ◽  
Author(s):  
Fabian Placzek ◽  
Eliana Cordero Bautista ◽  
Simon Kretschmer ◽  
Lara M. Wurster ◽  
Florian Knorr ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Imaging System ◽  
Ex Vivo ◽  
Large Field ◽  
Optical Coherence ◽  
Fiber Optic Probe ◽  
Optic Probe ◽  
Muscle Invasive

Characterization of bladder biopsies, using a combined fiber optic probe-based optical coherence tomography and Raman spectroscopy imaging system that allows a large field-of-view imaging and detection and grading of cancerous bladder lesions.

scholarly journals Optimization of X-ray Investigations in Dentistry Using Optical Coherence Tomography

Sensors ◽  
2021 ◽  
Vol 21 (13) ◽  
pp. 4554
Author(s):  
Ralph-Alexandru Erdelyi ◽  
Virgil-Florin Duma ◽  
Cosmin Sinescu ◽  
George Mihai Dobre ◽  
Adrian Bradu ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Radiation Dose ◽  
Imaging Techniques ◽  
Image Resolution ◽  
Optical Coherence ◽  
X Rays ◽  
High Quality ◽  
X Ray

The most common imaging technique for dental diagnoses and treatment monitoring is X-ray imaging, which evolved from the first intraoral radiographs to high-quality three-dimensional (3D) Cone Beam Computed Tomography (CBCT). Other imaging techniques have shown potential, such as Optical Coherence Tomography (OCT). We have recently reported on the boundaries of these two types of techniques, regarding. the dental fields where each one is more appropriate or where they should be both used. The aim of the present study is to explore the unique capabilities of the OCT technique to optimize X-ray units imaging (i.e., in terms of image resolution, radiation dose, or contrast). Two types of commercially available and widely used X-ray units are considered. To adjust their parameters, a protocol is developed to employ OCT images of dental conditions that are documented on high (i.e., less than 10 μm) resolution OCT images (both B-scans/cross sections and 3D reconstructions) but are hardly identified on the 200 to 75 μm resolution panoramic or CBCT radiographs. The optimized calibration of the X-ray unit includes choosing appropriate values for the anode voltage and current intensity of the X-ray tube, as well as the patient’s positioning, in order to reach the highest possible X-rays resolution at a radiation dose that is safe for the patient. The optimization protocol is developed in vitro on OCT images of extracted teeth and is further applied in vivo for each type of dental investigation. Optimized radiographic results are compared with un-optimized previously performed radiographs. Also, we show that OCT can permit a rigorous comparison between two (types of) X-ray units. In conclusion, high-quality dental images are possible using low radiation doses if an optimized protocol, developed using OCT, is applied for each type of dental investigation. Also, there are situations when the X-ray technology has drawbacks for dental diagnosis or treatment assessment. In such situations, OCT proves capable to provide qualitative images.

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