Volumetric stimulated Raman imaging with a high-speed deformable mirror (Conference Presentation)

2019 ◽  
Author(s):  
Peng Lin ◽  
Hongli Ni ◽  
Huate Li ◽  
Fengyuan Deng ◽  
Nicholas A. Vickers ◽  
...  
Keyword(s):  
High Speed ◽  
Raman Imaging ◽  
Deformable Mirror ◽  
Stimulated Raman

Stimulated Raman Imaging Reveals Aberrant Lipogenesis as a Metabolic Marker for Azole-ResistantCandida albicans

2017 ◽  
Vol 89 (18) ◽  
pp. 9822-9829 ◽  
Author(s):  
Caroline W. Karanja ◽  
Weili Hong ◽  
Waleed Younis ◽  
Hassan E. Eldesouky ◽  
Mohamed N. Seleem ◽  
...  
Keyword(s):  
Raman Imaging ◽  
Metabolic Marker ◽  
Stimulated Raman

scholarly journals Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering

2019 ◽  
Vol 116 (32) ◽  
pp. 15842-15848 ◽  
Author(s):  
Yuta Suzuki ◽  
Koya Kobayashi ◽  
Yoshifumi Wakisaka ◽  
Dinghuan Deng ◽  
Shunji Tanaka ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Cancer Biology ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Fluorescent Labeling ◽  
Label Free ◽  
Imaging Flow Cytometry ◽  
Stimulated Raman

Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural, and morphological phenotypes of numerous living cells to provide systematic insights into biological processes. However, its utility is constrained by its requirement of fluorescent labeling for phenotyping. Here we present label-free chemical imaging flow cytometry to overcome the issue. It builds on a pulse pair-resolved wavelength-switchable Stokes laser for the fastest-to-date multicolor stimulated Raman scattering (SRS) microscopy of fast-flowing cells on a 3D acoustic focusing microfluidic chip, enabling an unprecedented throughput of up to ∼140 cells/s. To show its broad utility, we use the SRS imaging flow cytometry with the aid of deep learning to study the metabolic heterogeneity of microalgal cells and perform marker-free cancer detection in blood.

scholarly journals Extended depth-of-field microscopy with a high-speed deformable mirror

2017 ◽  
Vol 42 (5) ◽  
pp. 995 ◽  
Author(s):  
William J. Shain ◽  
Nicholas A. Vickers ◽  
Bennett B. Goldberg ◽  
Thomas Bifano ◽  
Jerome Mertz
Keyword(s):  
High Speed ◽  
Deformable Mirror ◽  
Depth Of Field ◽  
Extended Depth Of Field

High-resolution, high-speed confocal Raman imaging

2008 ◽  
Vol 48 (1) ◽  
pp. 22-27 ◽  
Author(s):  
Thomas Dieing ◽  
Olaf Hollricher
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Raman Imaging ◽  
Confocal Raman

High-speed closed-loop dual deformable-mirror phase-diversity testbed

2007 ◽  
Author(s):  
James A. Georges III ◽  
Pam Dorrance ◽  
Kurt Gleichman ◽  
Julie Jonik ◽  
Dean Liskow ◽  
...  
Keyword(s):  
High Speed ◽  
Closed Loop ◽  
Deformable Mirror ◽  
Phase Diversity

High-speed deformable mirror system

1982 ◽  
Vol 21 (4) ◽  
pp. 589 ◽  
Author(s):  
R. H. Freeman ◽  
H. R. Garcia
Keyword(s):  
High Speed ◽  
Mirror System ◽  
Deformable Mirror

scholarly journals High-speed hyperspectral Raman imaging for label-free compositional microanalysis

2013 ◽  
Vol 4 (11) ◽  
pp. 2376 ◽  
Author(s):  
Ji Qi ◽  
Jingting Li ◽  
Wei-Chuan Shih
Keyword(s):  
High Speed ◽  
Raman Imaging ◽  
Label Free
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