Rapid detection of antibiotic-resistant bacteria at the single-cell level using two-photon excitation fluorescence and coherent anti-Stokes Raman scattering microscopy (Conference Presentation)

2020 ◽  
Author(s):  
Chi Zhang ◽  
Jungeun Won ◽  
Stephen A. Boppart
Keyword(s):  
Raman Scattering ◽  
Single Cell ◽  
Rapid Detection ◽  
Resistant Bacteria ◽  
Single Cell Level ◽  
Cell Level ◽  
Antibiotic Resistant ◽  
Two Photon ◽  
Photon Excitation ◽  
Anti Stokes

Singlet oxygen and ROS in a new light: low-dose subcellular photodynamic treatment enhances proliferation at the single cell level

2014 ◽  
Vol 13 (9) ◽  
pp. 1235-1240 ◽  
Author(s):  
Alfonso Blázquez-Castro ◽  
Thomas Breitenbach ◽  
Peter R. Ogilby
Keyword(s):  
Reactive Oxygen Species ◽  
Laser Beam ◽  
Hela Cells ◽  
Low Dose ◽  
Single Cell Level ◽  
Oxygen Species ◽  
Cell Level ◽  
Photodynamic Treatment ◽  
Two Photon ◽  
Photon Excitation

Two-photon excitation of a sensitizer with a focused laser beam was used to create a spatially-localized subcellular population of reactive oxygen species, ROS, stimulating proliferation in single HeLa cells.

In vivo Two-Photon Imaging of Energy Metabolism at Single Cell Level using Genetically Encoded Sensors

2015 ◽  
Author(s):  
Philipp Maechler ◽  
Matthias T. Wyss ◽  
Jillian Stobart ◽  
Marc Zuend ◽  
Felipe Barros ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Two Photon ◽  
Genetically Encoded Sensors ◽  
Photon Imaging ◽  
Two Photon Imaging
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